| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,237,258 | T→C | G90G (GGA→GGG) | cvrA ← | potassium/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,237,258 | 0 | T | C | 95.5% | 70.6 / ‑3.0 | 22 | G90G (GGA→GGG) | cvrA | potassium/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (11/10); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
CATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCC > NZ_CP009273/1237128‑1237392 | caTCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGc < 1:554377/139‑1 (MQ=255) tAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGc < 1:598159/139‑1 (MQ=255) cgcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCt > 1:740093/1‑138 (MQ=255) cGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGt < 2:71111/139‑1 (MQ=255) ttCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCccgccg > 1:660952/1‑139 (MQ=255) tcaatcaaATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGt > 2:667195/1‑139 (MQ=255) ccACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGt > 1:836317/1‑102 (MQ=255) ccACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGt < 2:836317/102‑1 (MQ=255) ccACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGc > 2:568072/1‑138 (MQ=255) ccgccATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGtt < 1:826289/139‑1 (MQ=255) ccATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGttact < 2:124657/139‑1 (MQ=255) gTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAgg > 2:62775/1‑139 (MQ=255) aCCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAg < 1:555175/139‑1 (MQ=255) agGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGt > 2:243981/1‑138 (MQ=255) gCACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCaaa < 1:243981/139‑1 (MQ=255) cACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGa < 2:530841/122‑1 (MQ=255) cACGCCCAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGa > 1:530841/1‑122 (MQ=255) cccAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGa < 1:492250/139‑1 (MQ=255) ccAGCGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGa > 2:313224/1‑138 (MQ=255) cGTCGCCAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATAccg > 2:570619/1‑139 (MQ=255) ccAGCGACAGTGCCGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGAc < 1:62775/139‑1 (MQ=255) ccGGCCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTcc > 1:18168/1‑139 (MQ=255) | CATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCC > NZ_CP009273/1237128‑1237392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |