| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,399,853 | A→G | T286A (ACC→GCC) | abgR → | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,399,853 | 0 | A | G | 93.8% | 95.6 / ‑0.3 | 32 | T286A (ACC→GCC) | abgR | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (12/18); total (13/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
TGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGC > NZ_CP009273/1399740‑1399963 | tGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCAt < 1:137165/123‑1 (MQ=255)tGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCAt > 2:137165/1‑123 (MQ=255)tGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTACTCACGCAATTCCg > 2:597820/1‑139 (MQ=255)tGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCg > 1:5779/1‑139 (MQ=255) ggCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGc < 1:481272/139‑1 (MQ=255) cTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGc > 2:481272/1‑137 (MQ=255) tGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTa > 1:275883/1‑139 (MQ=255) ggACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGc < 1:800818/139‑1 (MQ=255) ggACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGc < 1:693318/139‑1 (MQ=255) ggACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGc < 2:323843/139‑1 (MQ=255) aCAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCa > 1:62179/1‑138 (MQ=255) gTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACt > 2:128925/1‑139 (MQ=255) gCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGc < 2:278834/101‑1 (MQ=255) gCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGc > 1:278834/1‑101 (MQ=255) ttAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATa < 1:128925/139‑1 (MQ=255) ttAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATACCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATa < 2:515828/139‑1 (MQ=255) tAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATaaaaaaa > 2:866640/1‑136 (MQ=255) aGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGcc < 1:485999/139‑1 (MQ=255) ggCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGa < 1:423108/139‑1 (MQ=255) ccTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGAct < 1:366301/139‑1 (MQ=255) ttagtTGATTCAGCGGCGTGATAGTCGCCAGACACCCCTGGCCGCGTCATTAATCACGCTATTCCTGCGTGAATGCGGCTATCGGCAAAGTTAAAAATGTATAAAAAAATAGAGTCTGTCGATCTCCGCCAGAcgct < 2:607571/137‑1 (MQ=255) attTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACa < 2:252004/138‑1 (MQ=255) attTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACa < 2:446030/138‑1 (MQ=255) aTTCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTaca < 1:866640/138‑1 (MQ=255) ttCAGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTacaca > 1:312905/1‑139 (MQ=255) cAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCa > 1:55119/1‑46 (MQ=255) cAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCa < 2:55119/46‑1 (MQ=255) aGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGAc > 1:558922/1‑112 (MQ=255) aGCGGCGTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGAc < 2:558922/112‑1 (MQ=255) gcggcgTGATAGTCGCCAGACACCACTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACAcagc < 1:135693/139‑1 (MQ=255) cacTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGAc > 1:768004/1‑88 (MQ=255) cacTGGCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGAc < 2:768004/88‑1 (MQ=255) aCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATaga < 1:796394/73‑1 (MQ=255) aCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATaga > 2:796394/1‑73 (MQ=255) | TGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGC > NZ_CP009273/1399740‑1399963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |