| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,506,028 | G→A | A40T (GCC→ACC) | ydcS → | ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,506,028 | 0 | G | A | 92.6% | 99.6 / NA | 27 | A40T (GCC→ACC) | ydcS | ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/11); minor base C (0/2); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACAGGAGTAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGGTGAATGTGAAAACCGCCGCGACTTCCGATGAAATGGTCAGT > NZ_CP009273/1505893‑1506153 | cGACAGGAGTAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCt < 1:492278/139‑1 (MQ=255) aCAGGAGTAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTgg < 2:315750/139‑1 (MQ=255) cAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGa > 2:467552/1‑139 (MQ=255) agcagcCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACaa > 1:620284/1‑138 (MQ=255) gcCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGa < 1:420951/139‑1 (MQ=255) ccTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGAc > 1:743501/1‑139 (MQ=255) gcTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACg < 2:159313/139‑1 (MQ=255) aGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGt > 2:620948/1‑139 (MQ=255) gCATGACAATAATGACCGCTCACGCCGCCGAATCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGtt > 2:439010/1‑139 (MQ=255) cAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGaaa > 2:423072/1‑138 (MQ=255) taatGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGa > 2:263722/1‑115 (MQ=255) taatGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGa < 1:263722/115‑1 (MQ=255) gACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGa > 1:82007/1‑111 (MQ=255) gACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGa < 2:82007/111‑1 (MQ=255) cGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGa > 1:547437/1‑108 (MQ=255) cGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGa < 2:547437/108‑1 (MQ=255) gCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACa > 2:833224/1‑89 (MQ=255) gCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACa < 1:833224/89‑1 (MQ=255) cACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTgcg > 1:546203/1‑139 (MQ=255) aCGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTgcg < 2:145027/138‑1 (MQ=255) gccgccGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCgg < 1:467552/139‑1 (MQ=255) aaCCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGGTGAAtgt > 2:607647/1‑139 (MQ=255) aCCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGGTGAATGTGAAAACCGCCGCGACTTCCGAt > 2:264663/1‑139 (MQ=255) ccGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACa < 2:165876/53‑1 (MQ=255) ccGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACa > 1:165876/1‑53 (MQ=255) gCGACTGGATATTATCCCCTGGCCGGGATACATCGAACGCGGACAAACTGAAAAACAATACGACGGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGGTGAATGTGAAAACCGCCGCGACTTCCGATGAAATGGTc < 2:422935/139‑1 (MQ=255) agccagtattctCCCCTGGCCGGGATACAGCGAACGCGGACAAACTGATAAACAATCCGCCTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGTTGAATGTGAAAACCGCCGCGACTTCCGATGAAATGGTCAGt < 2:770348/133‑1 (MQ=255) | CGACAGGAGTAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGGTGAATGTGAAAACCGCCGCGACTTCCGATGAAATGGTCAGT > NZ_CP009273/1505893‑1506153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |