Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,542,720 A→G T355A (ACT→GCT)  fdnG → formate dehydrogenase‑N subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,542,7200AG96.9% 108.6 / ‑3.3 32T355A (ACT→GCT) fdnGformate dehydrogenase‑N subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (17/14);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

CTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCC  >  NZ_CP009273/1542588‑1542842
                                                                                                                                    |                                                                                                                          
ctgGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATc                                                                                                                      >  2:53878/1‑139 (MQ=255)
ctgGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATc                                                                                                                      >  2:48327/1‑139 (MQ=255)
   gTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCcgc                                                                                                                   <  2:656085/139‑1 (MQ=255)
    tGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCcgcg                                                                                                                  <  2:216470/139‑1 (MQ=255)
      cGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                                <  1:342939/139‑1 (MQ=255)
      cGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                                <  1:364460/139‑1 (MQ=255)
      cGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                                <  1:629495/139‑1 (MQ=255)
      cGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                                <  2:120672/139‑1 (MQ=255)
        tgatgaTTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCcgcg                                                                                                                  >  1:269509/1‑135 (MQ=255)
        tgatgaTTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCcgcg                                                                                                                  <  2:269509/135‑1 (MQ=255)
                                tCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCAc                                                                                      >  2:801666/1‑139 (MQ=255)
                                tCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCAc                                                                                      >  2:844050/1‑139 (MQ=255)
                                    ttCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGtt                                                                                   <  1:801666/138‑1 (MQ=255)
                                         cGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGc                                                                             >  1:689514/1‑139 (MQ=255)
                                         cGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGc                                                                             >  1:719513/1‑139 (MQ=255)
                                         cGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGc                                                                             >  1:112997/1‑139 (MQ=255)
                                           gCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTa                                                                           <  1:667505/139‑1 (MQ=255)
                                              acgacgCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGaaa                                                                                            >  2:384931/1‑119 (MQ=255)
                                              acgacgCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGaaa                                                                                            <  1:384931/119‑1 (MQ=255)
                                              acgacgCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGGGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTacac                                                                        >  2:773358/1‑139 (MQ=255)
                                                                       tAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGc                                               >  1:607150/1‑139 (MQ=255)
                                                                                   gAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCaa                                     >  2:820476/1‑137 (MQ=255)
                                                                                   gAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCaa                                     >  2:507231/1‑137 (MQ=255)
                                                                                      cTATCAGCTCGCTGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGcc                                <  1:773358/139‑1 (MQ=255)
                                                                                        aTCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                                <  2:850061/57‑1 (MQ=255)
                                                                                        aTCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                                >  1:850061/1‑57 (MQ=255)
                                                                                         tCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGa                              <  1:48327/138‑1 (MQ=255)
                                                                                                gATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCt                        <  1:407081/137‑1 (MQ=255)
                                                                                                 aTGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGa                      >  2:359822/1‑138 (MQ=255)
                                                                                                      aaCGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAgtgt                >  2:159107/1‑139 (MQ=255)
                                                                                                       aCGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAgtgtg               >  2:52714/1‑139 (MQ=255)
                                                                                                                    aCGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGcc  <  2:311158/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                          
CTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCC  >  NZ_CP009273/1542588‑1542842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑