New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP009273	281585 =	203 (4.410)	8 (0.190)	8/228	1.7	4.2%	coding (480/1383 nt)	yagG	MFS transporter
	?	NZ_CP009273	281607 =	177 (4.120)					coding (502/1383 nt)	yagG	MFS transporter
Rejected: Frequency below/above cutoff threshold.

AAAGGTGTAGCGCTCTTTGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP009273/281729‑281585 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgccgctgGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGC  >  NZ_CP009273/281607‑281744                                                                                                                                                                                                                                                                                             AAAGGTGTAGCGCTCTTTGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGAT                                                                                                                                                   <  1:169113/138‑1  AAGGTGTAGCGCTCTTTGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGC                                                                                                                                                 <  1:700422/139‑1   AGGTGTAGCGCTCTTTGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCC                                                                                                                                                >  2:42347/1‑139    GGTGTAGCGCTCTTTGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCG                                                                                                                                               <  1:816149/139‑1     GTGTAGCGCTCTTTGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGC                                                                                                                                              >  1:455951/1‑139      TGTAGCGCTCTTTGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCT                                                                                                                                             >  1:122998/1‑139      TGTAGCGCTCTTTGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCT                                                                                                                                             >  1:812129/1‑139          gctCTCTTTGGTCGTGAAGAAGTAGACGTAGAGCAGCACCACGCCGCGCAGCCCCAGCACGCGCATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTG                                                                                                                                         <  2:543403/136‑1                  TGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCAT                                                                                                                                    <  1:638392/136‑1                                   ACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGC                                                                                                                <  1:67041/139‑1                                           CAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGC                                                                                                        <  1:554123/139‑1                                             GCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGG                                                                                                                      <  2:120224/123‑1                                             GCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGG                                                                                                                      >  1:120224/1‑123                                                       GCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCC                                                                                            <  2:522555/139‑1                                                                       CACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGAT                                                                                                                                                   <  2:200665/68‑1                                                                       CACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGAT                                                                                                                                                   >  1:200665/1‑68                                                                                    AGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGT                                                               <  1:771890/139‑1                                                                                                                                            CCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGC       >  2:393862/1‑139                                                                                                                                            CCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAG        <  2:314372/138‑1                                                                                                                                                 GGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGC  >  1:665659/1‑139                                                                                                                                                                                                                                                                                             AAAGGTGTAGCGCTCTTTGGTCGTGAAGAAGCAGACGTAGAGCAGCACCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP009273/281729‑281585 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgccgctgGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGC  >  NZ_CP009273/281607‑281744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.