Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273229,6010GA44.4% 26.8 / 12.2 27S281F (TCC→TTC) mltDmurein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base G (15/0);  new base A (0/12);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 5.75e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGG‑CACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCC  >  NZ_CP009273/229472‑229738
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aTTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGG‑CACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCg                                                                                                                                  >  2:497746/1‑139 (MQ=255)
                agaatccACGCATTTAATCTGC‑TTCTTTTTTGGCCACCACCCCGTCCTGCGGGCCACTTGCCCCCGGCGGGGACCCTTTCCCGCCGGCGTTAATTTTTTCCATTTTGCTGACGAAAACCCCCGCCATATCTGCTACCtt                                                                                                                  <  2:833340/133‑1 (MQ=255)
                      cACGCAGTTGATCTGCATGCTTCTTTGG‑CACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCAt                                                                                                            >  1:103354/1‑139 (MQ=255)
                           agtttttCTGCTTGTTTTTTGGC‑CCCCATCCCGTTCGGCGGCCCACTGCCGCCCAGCGTGGTGTTTTTTCCCCCAGCGTTGAATGTCTTCAGTTTGCGGACGAAAACCCCCGCCATATCTGCTACCTTCGCCATTTCaa                                                                                                       <  1:901979/133‑1 (MQ=255)
                                     cttGCTTTTTTGG‑CCCCACCGCGTGCTGCGGCCCTCTGGCGCCCGGCGGGGCGCTTTTTCCGCCAGTGTTGAATTTCTTCGGCTTGCGGACGAAAACCCCCGCCATATCTGCTACCTTCGCCATTTCAACCggg                                                                                                  <  2:893406/132‑1 (MQ=255)
                                     cATGCTTCTTTGG‑CACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGgctgct                                                                                             >  2:311383/1‑139 (MQ=255)
                                           tcttTGG‑CACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTg                                                                                       >  1:14898/1‑139 (MQ=255)
                                                       aTCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACgcgc                                                                              >  1:455273/1‑137 (MQ=255)
                                                       aTCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACgcgc                                                                              >  2:896901/1‑137 (MQ=255)
                                                        tCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACgcgc                                                                              >  1:164245/1‑136 (MQ=255)
                                                            gtccggCGGCCCCCTTCCGCCCGGTTGGGAGCTTTTCCCCCCAGCTTTAATTGTCTCCAGCTTGCGGACGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCa                                                                       <  1:514189/134‑1 (MQ=255)
                                                            gtccTGCGGCCCACTTCCCCCCAGCGTGGAGCTTTTTCCCCCAGCGTTGATTGTCTTCAGTTTGCTGACGAAATCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCa                                                                       <  1:867060/136‑1 (MQ=255)
                                                                  cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCtt                                                                 >  1:590570/1‑139 (MQ=255)
                                                                  cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCtt                                                                 >  2:305283/1‑139 (MQ=255)
                                                                  cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCtt                                                                 >  1:590242/1‑139 (MQ=255)
                                                                  cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCtt                                                                 >  1:250648/1‑139 (MQ=255)
                                                                  cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCAAGAGCACGGCtt                                                                 >  2:834931/1‑139 (MQ=255)
                                                                    ggCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCttt                                                                >  1:544810/1‑138 (MQ=255)
                                                                      cccctcgcgcCCGGGGGGGAGCTTTTTCCCCCGGCTTTAAATGTTTGCAGCTGGCGGAGGAAAACCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCAt                                                             <  1:795689/133‑1 (MQ=255)
                                                                      ccACTGCCGCCGAGGGTGGACTCTTTCCCGCGGGTGTTTAATGTCTTCAGCTTGCTGACGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCAt                                                             <  2:461185/139‑1 (MQ=255)
                                                                                    cGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGa                                               >  1:37023/1‑139 (MQ=255)
                                                                                        gatccTTTTCCGCCAGCTTTTATTTCCTTCATCTTGCGAACGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTa                                           <  2:637001/136‑1 (MQ=255)
                                                                                                    ccggCGTTGAATTTCTTCCGTTTGCGGACGAAAACCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCt                               <  1:336207/136‑1 (MQ=255)
                                                                                                        cGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCt                           >  2:72153/1‑139 (MQ=255)
                                                                                                              ttGTCTTCAGTTTGCGGAGGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGttttt                     <  2:37023/138‑1 (MQ=255)
                                                                                                                     cAGCTTGCGGCGGAAAACCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAata              <  2:844606/139‑1 (MQ=255)
                                                                                                                      aGCTTGCGGAGGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAatat             <  2:503070/139‑1 (MQ=255)
                                                                                                                                 ggAACTCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGcc  <  1:314255/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                         
ATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGG‑CACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCC  >  NZ_CP009273/229472‑229738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑