BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	349,999	0	T	G	42.9%	219.4 / 32.8	127	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base T (16/56); new base G (54/0); total (71/56)
Fisher's exact test for biased strand distribution p-value = 1.50e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 129 total aligned reads displayed.

ACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTT  >  NZ_CP009273/349864‑350135

aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGa                                                                                                                                         >  2:319820/1‑138 (MQ=255)

  gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg                                                                                                                                      <  1:662237/139‑1 (MQ=255)

            aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAGGCGCTgg                                                                                                                             >  2:267010/1‑137 (MQ=255)

             tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaaa                                                                                                                           <  2:850091/139‑1 (MQ=255)

             tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                            <  1:267010/138‑1 (MQ=255)

                  ttATTCTCGGGCGCACTGACGATGTAATTAACGTCGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCTGGAAggggg                                                                                                                      >  2:761144/1‑137 (MQ=255)

                   tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCa                                                                                                                      <  1:95847/138‑1 (MQ=255)

                    aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGCGCCGGAAGGGGAgg                                                                                                                    >  2:510399/1‑139 (MQ=255)

                    aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGCAAGATCGGCTGCAAggggggg                                                                                                                    >  2:158045/1‑135 (MQ=255)

                          gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                               <  1:536826/138‑1 (MQ=255)

                          gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGGCTGGAAGGGGAggtggggg                                                                                                              >  2:542834/1‑139 (MQ=255)

                          gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAgggggggg                                                                                                              >  2:393361/1‑139 (MQ=255)

                           ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAAAGCGCTGAAAGGggcggtggcggt                                                                                                             >  1:92551/1‑139 (MQ=255)

                           ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAggtggcggg                                                                                                             >  1:695249/1‑138 (MQ=255)

                           ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGCAAGGGCAggggggggg                                                                                                             >  2:221829/1‑138 (MQ=255)

                               cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAggggggggggggg                                                                                                         >  2:727221/1‑137 (MQ=255)

                                  tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGGGGGGGCGgtt                                                                                                      >  1:305369/1‑139 (MQ=255)

                                    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑t                                                                                                   <  1:726935/139‑1 (MQ=255)

                                    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑t                                                                                                   <  1:112759/139‑1 (MQ=255)

                                    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑t                                                                                                   <  2:700782/139‑1 (MQ=255)

                                    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑t                                                                                                   <  1:499340/139‑1 (MQ=255)

                                      gacagaaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCa                                                                                                 <  1:809105/134‑1 (MQ=255)

                                      gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGGCTGGAAGGGGAGGGGGGGGGGGCGGTTG‑TCa                                                                                                 >  2:386062/1‑139 (MQ=255)

                                        tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTG‑TCaat                                                                                               >  2:750162/1‑137 (MQ=255)

                                          tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGCGGGGGGGTTTGTTCATTc                                                                                              >  2:427887/1‑139 (MQ=255)

                                            aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCCGGTGGGGGGGGGGGTTG‑TGCTTCCg                                                                                            >  2:755931/1‑138 (MQ=255)

                                            aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGAAAGGGGAGGTGGCGGGGGCGTTTG‑TCATTTCg                                                                                            >  1:99308/1‑138 (MQ=255)

                                            aTTAACCTTGCCGGGCATCGGCTGGGGAAGAGCGCGATTGCAGAGAGTATCTCCCGCCAGCCGGGTGTCGCCGAAGTGGCTGTGGTTGGGGTCAAAGATGAGCTGACAGGGCAGGTGGCGGTGGCGCTTG‑TCCTGCCGa                                                                                           <  2:630087/139‑1 (MQ=255)

                                                  gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGGGGTTG‑TCATTCCGGAaga                                                                                       >  1:288455/1‑137 (MQ=255)

                                                    tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGGCCGGAAGGGGAGGTGGCGGGGGGGGTTG‑GCAATTCCTAagagagg                                                                                   >  2:666972/1‑138 (MQ=255)

                                                    tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGTGGTGCAAAATCTCGTGGAAGGGCAGGTCGCCGGGGCCGTTG‑TCTTTCCCGAAGAGGGc                                                                                   >  1:189737/1‑139 (MQ=255)

                                                     gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTG‑TCCATCCGAAagagggg                                                                                   >  1:113185/1‑135 (MQ=255)

                                                     gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGTGGAAGATGCGCTTAAAGGGCAGGGGGGGGGGGGGGTTG‑TCATTTCGTAAGGGAGCg                                                                                  >  2:675110/1‑139 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:680364/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  2:61098/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:892569/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:887430/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  2:143795/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  2:371137/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:605403/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:393361/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:489972/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:550218/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:348433/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:725052/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:278236/139‑1 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGGGGGGGGGGTTG‑TCATTTCGGAagaggtgtc                                                                                 >  2:809105/1‑134 (MQ=255)

                                                      ccGGGCATCGGCTGGGGACGCGCGAGAGTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  2:797051/139‑1 (MQ=255)

                                                        gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACa                                                                               <  2:495746/139‑1 (MQ=255)

                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGa                                                                        <  1:363688/139‑1 (MQ=255)

                                                               acagggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGa                                                                        <  1:271592/135‑1 (MQ=255)

                                                                gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                       <  1:354581/139‑1 (MQ=255)

                                                                    gggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGa                                                                     <  2:560921/137‑1 (MQ=255)

                                                                         gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAt                                                              <  1:533243/139‑1 (MQ=255)

                                                                         gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCGGTTG‑TCATTTCGAAAAAGAGCGAAAGTCTGGAAGATCGGGAt                                                              >  2:656562/1‑139 (MQ=255)

                                                                          cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                             >  1:749660/1‑139 (MQ=255)

                                                                          cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGTGGGGGTTG‑TCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGTGAtg                                                             >  1:730210/1‑139 (MQ=255)

                                                                          cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTGAAAGGGCAGGGGGCGGTGGCGTTTG‑TCCTTCCGAAAGAGAGCGACAGGCTGGAAGAGCggggtg                                                             >  2:172726/1‑134 (MQ=255)

                                                                          cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAAATGCGCTGGAAGGGCCGGGGGCGGGGGCGGTTT‑TCATTCCGGAAGCGGGCGGCAGGCTGGGAGATtgtggcg                                                             >  2:71576/1‑136 (MQ=255)

                                                                               gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                        <  1:668196/139‑1 (MQ=255)

                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcgc                                                       <  1:639051/139‑1 (MQ=255)

                                                                                 ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                      <  1:192856/139‑1 (MQ=255)

                                                                                 ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCAGTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                      <  1:804043/139‑1 (MQ=255)

                                                                                    aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACt                                                    <  1:696709/138‑1 (MQ=255)

                                                                                         agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGGGGTTT‑TCTTTCCGGAAAAGAGCGACCGTCTGGAAGATTGTGGTGTGGGGCACTCGc                                                 >  2:490566/1‑136 (MQ=255)

                                                                                         agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGAAAGGGGAGGGGGCGGGGGCGGTTG‑TCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGGGGTGTGGGGCACTCGc                                                 >  2:420279/1‑136 (MQ=255)

                                                                                         agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGGTTG‑TCATTTCGAAAGAGAGCGGCAGTCTGGAAGATCGTGATGTGGGGCACTCGc                                                 >  2:668196/1‑136 (MQ=255)

                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAACGACGCGCTGGAAGGGCAGGTGGCGGGGGCGTTTG‑TCATTCCGGAAAAGAGCGACAGTCTGGAAGGTCGTGATGTGGCGCCCTCGCaa                                               >  2:4860/1‑136 (MQ=255)

                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGAGGGGGTGGCGGTTG‑TCTTTTCGAAAGAGAGCGACAGGCTGGGAGATCGTGATGTGGGGCACTCGCaa                                               >  2:745687/1‑136 (MQ=255)

                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGt                                                                                                                                           >  2:851202/1‑45 (MQ=255)

                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGa                                                                                           >  1:526391/1‑92 (MQ=255)

                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCaa                                               >  1:108761/1‑136 (MQ=255)

                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGGCAGGCTGGAAGATCGTGGTGTGGGGCACTCGcca                                               >  2:310577/1‑134 (MQ=255)

                                                                                                 cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                          <  1:310577/135‑1 (MQ=255)

                                                                                                 cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                       <  1:456467/138‑1 (MQ=255)

                                                                                                   gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACCGTCTGGAAGAACGTGATGTGGCGCACTCGCAAGAGAAGGCGAt                                    >  1:350630/1‑139 (MQ=255)

                                                                                                   gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGGGCTGAACGGGCAGGCGGGGGGGGTGGTTG‑TTGTTTCGAAAGAGAGCGACAGGCTGGGAGATAGGGATGGAGCGCAACCGCCAGAGAAAGGGAt                                    >  2:780622/1‑139 (MQ=255)

                                                                                                   gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGTGGCGGGGGCGGTTG‑TCCTTCCGAAAGAGAGCGGCCGTCTGGGAGATCGGGGTGTGGCGCACCCGCCAGAGAAGGCggt                                    >  2:598041/1‑137 (MQ=255)

                                                                                                   gCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGAAAGGGCAGGTGGCGGGGGCGTTTG‑TTATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGGGAt                                    >  1:575123/1‑139 (MQ=255)

                                                                                                       tCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGGGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                >  1:544402/1‑139 (MQ=255)

                                                                                                       tCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGGGGGGGCGGTTG‑TCATTTCGAAAGAGAGCGACAGTCTGGAAGAGCGGGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                >  2:233598/1‑139 (MQ=255)

                                                                                                       tCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGAGCTGGAAGGGCAGGGGGCGGGGGCGGTTG‑TCATTCCGGAAAAGAGGGGAAGTCTGGAAGATCGGGGTGTGGGGCACTCGCAAGAGAAAGCGGTTATg                                >  2:525447/1‑139 (MQ=255)

                                                                                                        ccGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                               >  2:432318/1‑139 (MQ=255)

                                                                                                         cGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                               <  1:902988/138‑1 (MQ=255)

                                                                                                         cGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                               <  1:420279/138‑1 (MQ=255)

                                                                                                         cGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                               <  2:284595/138‑1 (MQ=255)

                                                                                                                 gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtg                       <  1:386062/138‑1 (MQ=255)

                                                                                                                 gCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAGGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCtggtgg                      >  2:521310/1‑139 (MQ=255)

                                                                                                                 gCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                      >  1:176149/1‑139 (MQ=255)

                                                                                                                 gCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGGGGCGCACTCGCAAGAGAAGGGGATTATGGCGCcggggg                      >  2:788330/1‑139 (MQ=255)

                                                                                                                      aGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCACACTCGCAAGAGAAGTCGATTATGGCGCTGGTGGACAGc                 <  2:339096/139‑1 (MQ=255)

                                                                                                                      aGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGAATATGGGGCTGGTGGAGAGc                 >  2:54464/1‑139 (MQ=255)

                                                                                                                      aGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAAAGc                 >  1:872343/1‑139 (MQ=255)

                                                                                                                            ggtggtTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt           >  2:639191/1‑139 (MQ=255)

                                                                                                                                  tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 <  1:107370/63‑1 (MQ=255)

                                                                                                                                  tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGa                                                                                 >  2:107370/1‑63 (MQ=255)

                                                                                                                                   ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACt    >  1:296673/1‑139 (MQ=255)

                                                                                                                                     ggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                      >  1:469953/1‑87 (MQ=255)

                                                                                                                                     ggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                      <  2:469953/87‑1 (MQ=255)

                                                                                                                                     ggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt  >  1:814428/1‑139 (MQ=255)

ACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTG‑TCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTT  >  NZ_CP009273/349864‑350135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`