Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273353,5910TC45.9% 131.4 / 52.8 110K271E (AAA→GAA) cynRtranscriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base T (48/11);  new base C (0/50);  total (48/62)
Fisher's exact test for biased strand distribution p-value = 1.52e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 115 total aligned reads displayed.

AGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGC  >  NZ_CP009273/353457‑353728
                                                                                                                                           |                                                                                                                                              
aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgc                                                                                                                                           <  2:284801/139‑1 (MQ=255)
aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgc                                                                                                                                           <  2:905229/139‑1 (MQ=255)
  cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgccg                                                                                                                                         >  1:184641/1‑139 (MQ=255)
  cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgccg                                                                                                                                         >  1:185411/1‑139 (MQ=255)
  cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgccg                                                                                                                                         >  2:316438/1‑139 (MQ=255)
  cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgccg                                                                                                                                         >  1:42633/1‑139 (MQ=255)
  cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgccg                                                                                                                                         >  1:435238/1‑139 (MQ=255)
   gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgccg                                                                                                                                         >  1:329626/1‑138 (MQ=255)
    tAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑Ccgccgc                                                                                                                                        >  1:364803/1‑138 (MQ=255)
       cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGcaac                                                                                                                                     >  2:331731/1‑138 (MQ=255)
         cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGcaacaa                                                                                                                                   >  2:836908/1‑138 (MQ=255)
           tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAAc                                                                                                                                >  2:365145/1‑139 (MQ=255)
            cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAAcc                                                                                                                               >  2:698402/1‑139 (MQ=255)
            cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAAcc                                                                                                                               >  1:263858/1‑139 (MQ=255)
                  atcccAGCCGCAGACCTGTGTCAGCGCCTCCCGTGTTTCATTCCCCCAAACACCCGCGCTTTTACCCACCGCCATGTGAAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGCAAAAAAACCGCCGtt                                                                                                                         <  1:634830/136‑1 (MQ=255)
                  atcccACCCCCAGCCCTGTGCCAGCGGCTCCCGTGATTCATTCCCCCCAACACCCGCGCATTTACCCACCCCCATGTAAAAAAA‑GG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GTTTTTTTC‑CCGCCGAAACAAAACCGCCGtt                                                                                                                         <  2:364803/136‑1 (MQ=255)
                  aTTCCAGCCGCAGCCCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCATTTACCCACCGCCATGTGAAAAAA‑TG‑CCTT‑C‑GCGGGGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGAAAAAAAACCGCCGtt                                                                                                                         <  1:832600/139‑1 (MQ=17)
                  aTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCATTTACCCAACGCCATGTGCAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGCAACAAAACCGCCGtt                                                                                                                         <  1:243472/139‑1 (MQ=18)
                  aTTCCACCCGCAGCCCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCATTTACCCAACCCCATGTGAAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGCAAAAAAACCGCCGtt                                                                                                                         <  1:817661/139‑1 (MQ=18)
                   ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTtc                                                                                                                        >  1:661686/1‑139 (MQ=255)
                   ttCCAGCAGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GATATTTTT‑CCGCCGCAACAAAACCGCCGTtc                                                                                                                        >  2:911084/1‑139 (MQ=255)
                    tCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTtct                                                                                                                       >  2:713731/1‑139 (MQ=255)
                      cAGCCGCAGACCTGTGTCGGCGGCTACCGTGATTCATTCCCCCCAACACCCGCGATTTTACCCAGCGCCATGTGCAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCGGCCA‑GCTTTTTTC‑CCGCCGCAACAAAACCGCCGTtctc                                                                                                                      <  1:753209/138‑1 (MQ=18)
                         ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTtctctc                                                                                                                    >  1:632187/1‑137 (MQ=255)
                         ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTtctctc                                                                                                                    >  2:70597/1‑137 (MQ=255)
                          cgaagcccTGTGCCGGCGGCTCCCGTGATTCATTCCCGCCAACACCCGCGCATTTACCCACCCCAATGTAAAAAAA‑TG‑CCTC‑C‑GGGGCGGCTGTCGGCCA‑GTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCag                                                                                                                 <  1:331731/133‑1 (MQ=16)
                           gCACACCTGTGCCGGCGCCTCCCGTATTTCTTCCCCCCAAACACCCCCCCTTTTACCCACCGCTTTGTGAAAAAT‑TC‑CCTC‑C‑GCGGCGGCTTTCTCCCA‑TTTTTTTTC‑CCCCCGCAACAAAACCGCCGTTCTCTCCagt                                                                                                                <  2:322947/139‑1 (MQ=255)
                              gACTTGTGCCAGCGGCTACCGTGATTCATTTCCGCAAACACCCGCGCTTTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGTCGCAAAAAAACCGCCGTTCTCTCCagtagt                                                                                                             <  1:71253/139‑1 (MQ=21)
                                cttgtgCCAGCGCCTCCCGTGATTCTTTCCCGCAACCACCCGCGCTTTTACCCACCCCCTTGTG‑AAAAA‑AG‑CCTT‑CGGCGGGGGTTTTCTGCCG‑TTTTTTTTC‑CCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                           <  2:777944/137‑1 (MQ=255)
                                ccTGTTTCGGCGGCTCCCGGGTTTCATTCCCGCAAACACCCGCGATTTTATCCACCGCAAGGGGAAAAAA‑GG‑CCTT‑C‑GGGGCGGCTGTCGGCCA‑GTTTTTTTC‑CCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                           <  1:836908/139‑1 (MQ=255)
                                ccTGTGTCGGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCTTTTACCCACCGCCAGGTGAAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GTTATTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                           <  1:571728/139‑1 (MQ=18)
                                   gtgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgcc                                                                                                                                          <  2:889214/105‑1 (MQ=255)
                                   gtgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgcc                                                                                                                                          >  1:889214/1‑105 (MQ=255)
                                   gtgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                        >  2:87925/1‑139 (MQ=255)
                                   gtgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                        >  2:1974/1‑139 (MQ=255)
                                    tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                       >  2:795443/1‑139 (MQ=255)
                                    tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCCATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                       >  2:343934/1‑139 (MQ=255)
                                    tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCAATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                       >  2:187367/1‑139 (MQ=255)
                                      tCAGGGGCTCCCGTGATTCATTCCCGCCAACACCCGCGCATTTACCAAGCGCCATGTGAAAAAA‑TG‑CCTG‑C‑GCGGCGGCTGTCTGCCA‑GCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc                                                                                                     <  1:500621/139‑1 (MQ=21)
                                         gCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTCTCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGACA‑GCCATTTTT‑CCGCCGCAACAAAACCGCCGCTCTCTCTAGAAGTGGCggcgtac                                                                                                   >  2:492831/1‑133 (MQ=18)
                                         gCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggag                                                                                                  >  2:360095/1‑135 (MQ=255)
                                          cGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAagg                                                                                                 >  1:14476/1‑138 (MQ=255)
                                           ggCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggga                                                                                                   >  1:132080/1‑133 (MQ=255)
                                            gCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgc                                                                                                                                           >  2:559283/1‑95 (MQ=255)
                                            gCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑ccgc                                                                                                                                           <  1:559283/95‑1 (MQ=255)
                                              tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGTCGGGGCAAGA‑Ggaa                                                                                            >  2:124377/1‑139 (MQ=255)
                                              tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga‑gga                                                                                             >  1:234404/1‑136 (MQ=255)
                                               cccGTAATTCATTCCCCCCACCACCCGCGCATTAACCCACCCCAATGTGAAAAAA‑TG‑CCTC‑G‑GGGGCGGCT‑TCCGCCA‑GGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAAt                                                                                           <  2:263858/138‑1 (MQ=255)
                                                cctttaTTCTTCTCCACAAACACCCGCTCATTTCACCAACGCTATTTGAAAAAT‑TG‑CATT‑C‑GCGTGGTTTGTTTGCAA‑CCTTTTTTT‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAAt                                                                                           <  1:365145/133‑1 (MQ=255)
                                                        cATTCCCGCCAACACCCGCGCTTTTATCCAACGCCTTGTGCAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GTTTTTTTT‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑aa                                                                                 <  1:862252/138‑1 (MQ=255)
                                                         aTTCCCCCCAACACCCGCGCATTTACCCACCCCAATGTGCAAAAA‑TG‑CCTC‑C‑GCGGCGGCTTTCTGCCA‑GCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGc                                                                               <  1:878993/139‑1 (MQ=255)
                                                           tccccccaccaCCCGCGCATTTACCCAACGCCTTGTAAAAAAA‑TC‑CCTC‑C‑GCGGCGGCTGTCTCCCA‑TTTTTTTTC‑CCCCCACAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGc                                                                               <  2:419988/132‑1 (MQ=255)
                                                            cccGCCAACACCCGCGCATTTACCCACCGCCATGTGAAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGccc                                                                             <  1:732151/137‑1 (MQ=37)
                                                             cacccAACACCCGCGTATTAATCCAAGGTTATGCGAAAAAA‑TG‑TTTC‑G‑GGGGCGGGTGGCTGCCG‑GTTTTTTTG‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCg                                                                            <  2:185411/135‑1 (MQ=255)
                                                              ccccAACACCCGGCCTTTCACCCACCGCCATGTGCAAAAA‑TC‑CCTT‑C‑GCGGCGGTTGTCTCCCA‑GGTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTc                                                                          <  2:66617/137‑1 (MQ=255)
                                                              ccccAACACCCGCTCTTTTACCCAACGCCATGTGCAAAAA‑GG‑CCTT‑C‑GCGGGGGTTGTCTGCCA‑GTTTTTTTC‑CCGCCACACAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTc                                                                          <  1:477825/137‑1 (MQ=37)
                                                              ccccAAC‑ACCGCCCATTTTACCCACCCCAAGGGAAAAAATTC‑CCTC‑C‑GCGGGGGCTTTCTGCCA‑TTTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTc                                                                          <  2:5649/137‑1 (MQ=255)
                                                              cccaaacaccCGCCCATTTA‑CCAACCCCCGGGGCAAAAA‑AGCCCTC‑C‑GCGGGGGCTGTCGGCAA‑GCTTTTTTC‑CCGCCGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTc                                                                          <  2:213316/135‑1 (MQ=255)
                                                                          cgcATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGCGA‑GAAAAAAA‑ATT‑AAACCCCCCCTGTTTTGTggg                                                              >  2:212606/1‑138 (MQ=25)
                                                                              tttACCCAACGCCATGTGCAAAAA‑TG‑CTTC‑C‑GCGGCGGCTGTCTGCCA‑GTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAAt                                                           <  1:645086/138‑1 (MQ=39)
                                                                               agacccAACGCCATGTGAAAAAA‑TG‑CCTC‑C‑GGGGGGGTTGTCTGCCA‑GCTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  1:825148/135‑1 (MQ=255)
                                                                                tacccAACGCCTTGTGAAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑TCTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  2:358011/135‑1 (MQ=255)
                                                                                tacccAACGCCATGTGAAAAAA‑TG‑CTTC‑C‑GCGGCGGCTGTCTGCCA‑GCTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  1:212606/135‑1 (MQ=255)
                                                                                tacccAACGCCAGTGGAAAAAA‑CG‑CCTC‑G‑GCGGCGGCTTTCCGCCG‑GTTTTTTTC‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  2:184641/135‑1 (MQ=255)
                                                                                tATCCACCCCCATGTGAAAAAA‑TG‑CTTG‑C‑GCGGCGGTTGTCGGCCA‑GTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  1:87925/138‑1 (MQ=255)
                                                                                  tCCACCCCCATGTCAAAAAA‑GG‑CCTC‑C‑GCGGCGGTTGTCTGCAA‑GTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCgct                                                       <  2:588983/138‑1 (MQ=255)
                                                                                  cccAACGCCATGTGAAAAAA‑TG‑CCTC‑C‑GGGGCGGCTGTCTGCCA‑GTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCgct                                                       <  1:728752/137‑1 (MQ=255)
                                                                                  cccAACGCCATGTCAAAAAA‑GC‑CCTC‑C‑GCGGCGCCTTCCTGCCA‑TCTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCgct                                                       <  2:372454/137‑1 (MQ=255)
                                                                                    cacccccATGTGCAAAAA‑TG‑CCTC‑C‑GGGGCGGCTGTCTGCCA‑GCTATTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCgctg‑ct                                                   <  1:270431/134‑1 (MQ=255)
                                                                                       cGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGGAAAAAAA‑TTT‑AA‑ACCGCCCTGTTGTGGGGGGATTGCTG‑CTggg                                                >  2:728752/1‑138 (MQ=21)
                                                                                       cGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGA‑GAAAAAGC‑ATT‑TAACCCCCAACGTTGTGGGGGAAACGCTG‑CTGGt                                                >  1:346330/1‑139 (MQ=25)
                                                                                       cGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAAAAAC‑ATT‑TAACCCCCCATGTTTTGTGGCGATCGCCG‑CTGGt                                                >  1:69693/1‑139 (MQ=37)
                                                                                       cGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAAA‑GAAAAAGA‑ATT‑TAAACCGCCATGTTTTGTTGGGAACGCTG‑CTggg                                                >  1:521362/1‑138 (MQ=25)
                                                                                       cGCCATGT‑CAAAAA‑CT‑CCTTCC‑GCGGCTCTGGTCTCCCT‑TCTTTTTTC‑CCCCCTCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGt                                                <  2:833027/139‑1 (MQ=255)
                                                                                        cccATGTACAAAAA‑CC‑CCCC‑C‑GCGGCGCCTTCCTGCCA‑TTTTTTTTC‑CCGCCGCAACAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTa                                               <  2:74194/138‑1 (MQ=255)
                                                                                         ccATGTACAAAAA‑TG‑CTTC‑C‑GGGCCGGTTGTCTGCCG‑TTTTTTTTC‑CCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTaa                                              <  2:598470/139‑1 (MQ=255)
                                                                                           aTGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑GAAAAAACTTTT‑AACCCCCCCTTGTT‑TGTGGGAAACGCCG‑CTGGtgaa                                             >  1:784647/1‑135 (MQ=25)
                                                                                           aTGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGA‑GAAAAAAC‑TTT‑TATCCCCCCCTGTTTTTTGGCAATCGCTGCCTGGTac                                              >  1:93629/1‑137 (MQ=25)
                                                                                            tgtgCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAAAAGA‑TTT‑TAAACCCTCCAGTTGTGTGGGAATAGCCG‑CTggggaca                                            >  1:762960/1‑133 (MQ=25)
                                                                                            tgtgCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAGA‑GGAATAAA‑TTT‑TAAACCCCCCTGGTGTGTGGGCATCGCCG‑CCGGGAACa                                            >  1:129265/1‑138 (MQ=25)
                                                                                            tgtgCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGC‑AAAAAAAG‑TTT‑ATACACCGCCTGTTTTTTGGCTATCGCCT‑CTGGTaaaca                                           >  1:562926/1‑136 (MQ=25)
                                                                                              tgaaaaaa‑TG‑CCTG‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAAt                                          <  1:491229/135‑1 (MQ=255)
                                                                                                  aaaa‑TG‑CCTC‑G‑GGGGGGCCTGTC‑GCCAGGTTATTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGa                                     <  2:118416/139‑1 (MQ=255)
                                                                                                        g‑CCGT‑C‑GGGGCGGCTGTCCGCCG‑TTTTTTTCC‑CCGGCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAggg                                <  2:326039/139‑1 (MQ=255)
                                                                                                          ccTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGGAAAAAAT‑TTT‑AAACCC‑CCATGTTTTGTGGGGATCGCTG‑CTGGGGAAAAAGTGGGAAggg                                >  1:244733/1‑138 (MQ=255)
                                                                                                          ccTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGACA‑GAAAAAGC‑TTT‑AAAAACGCCTTGTTTTGTGGCGCAAGCTG‑CTGGTTACCAAGTggggaagg                                >  1:617512/1‑132 (MQ=255)
                                                                                                             t‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAAA‑GGAAAAAC‑TTTAAAGCCCG‑CCTGTTGTGTGGGAAATGCTG‑CTGGGAAAAAAGTGGGAAGGGGAgg                            >  1:378930/1‑138 (MQ=255)
                                                                                                                           tccgCCA‑GCTTTTTTC‑CCGCCGAAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  2:132080/134‑1 (MQ=255)
                                                                                                                           tCTGCCA‑TTTTTTTCC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  2:374387/137‑1 (MQ=255)
                                                                                                                           tCTGCCA‑GTTTTTTTT‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  1:254734/137‑1 (MQ=255)
                                                                                                                           tCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  1:713731/137‑1 (MQ=255)
                                                                                                                           tCTGCCA‑CTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  1:168935/137‑1 (MQ=255)
                                                                                                                           ccggCCA‑GTTATTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  1:402918/134‑1 (MQ=255)
                                                                                                                                 cacttATTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa           <  1:423214/135‑1 (MQ=255)
                                                                                                                                   gCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GGAAAAAC‑TTT‑TAACCCCTCCTGTTGTGTGGGGATCCCCG‑CTGGTGAAAAAATGGGAAGGGGAGTGGGGGGGAAGAGCTcccg          >  1:587031/1‑136 (MQ=255)
                                                                                                                                     atttttc‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc       <  2:206608/137‑1 (MQ=255)
                                                                                                                                      aTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc       <  1:344678/138‑1 (MQ=255)
                                                                                                                                       ttttc‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc       <  1:18706/137‑1 (MQ=255)
                                                                                                                                        tttc‑CCCCCGCAACAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcg     <  1:911084/138‑1 (MQ=255)
                                                                                                                                           t‑CCCCCCCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCgcgc  <  1:411854/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                              
AGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGC  >  NZ_CP009273/353457‑353728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑