BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	353,601	0	C	A	39.4%	95.2 / 45.8	99	L267F (TTG→TTT)	cynR	transcriptional regulator CynR
Reads supporting (aligned to +/- strand): ref base C (35/25); new base A (0/39); total (35/64)
Fisher's exact test for biased strand distribution p-value = 1.19e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 101 total aligned reads displayed.

CGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGC  >  NZ_CP009273/353464‑353728

cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGcaac                                                                                                                                     >  2:331731/1‑138 (MQ=255)

  cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGcaacaa                                                                                                                                   >  2:836908/1‑138 (MQ=255)

    tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAAc                                                                                                                                >  2:365145/1‑139 (MQ=255)

     cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAAcc                                                                                                                               >  2:698402/1‑139 (MQ=255)

     cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAAcc                                                                                                                               >  1:263858/1‑139 (MQ=255)

           atcccAGCCGCAGACCTGTGTCAGCGCCTCCCGTGTTTCATTCCCCCAAACACCCGCGCTTTTACCCACCGCCATGTGAAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGCAAAAAAACCGCCGtt                                                                                                                         <  1:634830/136‑1 (MQ=255)

           atcccACCCCCAGCCCTGTGCCAGCGGCTCCCGTGATTCATTCCCCCCAACACCCGCGCATTTACCCACCCCCATGTAAAAAAA‑GG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GTTTTTTTC‑CCGCCGAAACAAAACCGCCGtt                                                                                                                         <  2:364803/136‑1 (MQ=255)

           aTTCCAGCCGCAGCCCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCATTTACCCACCGCCATGTGAAAAAA‑TG‑CCTT‑C‑GCGGGGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGAAAAAAAACCGCCGtt                                                                                                                         <  1:832600/139‑1 (MQ=17)

           aTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCATTTACCCAACGCCATGTGCAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGCAACAAAACCGCCGtt                                                                                                                         <  1:243472/139‑1 (MQ=18)

           aTTCCACCCGCAGCCCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCATTTACCCAACCCCATGTGAAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGCAAAAAAACCGCCGtt                                                                                                                         <  1:817661/139‑1 (MQ=18)

            ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTtc                                                                                                                        >  1:661686/1‑139 (MQ=255)

            ttCCAGCAGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GATATTTTT‑CCGCCGCAACAAAACCGCCGTtc                                                                                                                        >  2:911084/1‑139 (MQ=255)

             tCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTtct                                                                                                                       >  2:713731/1‑139 (MQ=255)

               cAGCCGCAGACCTGTGTCGGCGGCTACCGTGATTCATTCCCCCCAACACCCGCGATTTTACCCAGCGCCATGTGCAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCGGCCA‑GCTTTTTTC‑CCGCCGCAACAAAACCGCCGTtctc                                                                                                                      <  1:753209/138‑1 (MQ=18)

                  ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTtctctc                                                                                                                    >  2:70597/1‑137 (MQ=255)

                  ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTtctctc                                                                                                                    >  1:632187/1‑137 (MQ=255)

                   cgaagcccTGTGCCGGCGGCTCCCGTGATTCATTCCCGCCAACACCCGCGCATTTACCCACCCCAATGTAAAAAAA‑TG‑CCTC‑C‑GGGGCGGCTGTCGGCCA‑GTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCag                                                                                                                 <  1:331731/133‑1 (MQ=16)

                   cGCAGCCCTGTGTCAGCGGCTCCCGTGATTAATTCCCGCCAACACCCGCGCATTTATCCACCGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTTTTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCag                                                                                                                 <  2:225940/139‑1 (MQ=18)

                    gCACACCTGTGCCGGCGCCTCCCGTATTTCTTCCCCCCAAACACCCCCCCTTTTACCCACCGCTTTGTGAAAAAT‑TC‑CCTC‑C‑GCGGCGGCTTTCTCCCA‑TTTTTTTTC‑CCCCCGCAACAAAACCGCCGTTCTCTCCagt                                                                                                                <  2:322947/139‑1 (MQ=255)

                       gACTTGTGCCAGCGGCTACCGTGATTCATTTCCGCAAACACCCGCGCTTTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGTCGCAAAAAAACCGCCGTTCTCTCCagtagt                                                                                                             <  1:71253/139‑1 (MQ=21)

                         cttgtgCCAGCGCCTCCCGTGATTCTTTCCCGCAACCACCCGCGCTTTTACCCACCCCCTTGTG‑AAAAA‑AG‑CCTT‑CGGCGGGGGTTTTCTGCCG‑TTTTTTTTC‑CCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                           <  2:777944/137‑1 (MQ=255)

                         ccTGTTTCGGCGGCTCCCGGGTTTCATTCCCGCAAACACCCGCGATTTTATCCACCGCAAGGGGAAAAAA‑GG‑CCTT‑C‑GGGGCGGCTGTCGGCCA‑GTTTTTTTC‑CCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                           <  1:836908/139‑1 (MQ=255)

                         ccTGTGTCGGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCTTTTACCCACCGCCAGGTGAAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GTTATTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                           <  1:571728/139‑1 (MQ=18)

                            gtgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                        >  2:87925/1‑139 (MQ=255)

                            gtgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                        >  2:1974/1‑139 (MQ=255)

                             tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                       >  1:374387/1‑139 (MQ=255)

                             tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                       >  2:795443/1‑139 (MQ=255)

                             tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCCATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                       >  2:343934/1‑139 (MQ=255)

                             tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCAATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                       >  2:187367/1‑139 (MQ=255)

                               tCAGGGGCTCCCGTGATTCATTCCCGCCAACACCCGCGCATTTACCAAGCGCCATGTGAAAAAA‑TG‑CCTG‑C‑GCGGCGGCTGTCTGCCA‑GCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc                                                                                                     <  1:500621/139‑1 (MQ=21)

                                  gCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTCTCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGACA‑GCCATTTTT‑CCGCCGCAACAAAACCGCCGCTCTCTCTAGAAGTGGCggcgtac                                                                                                   >  2:492831/1‑133 (MQ=18)

                                  gCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggag                                                                                                  >  2:360095/1‑135 (MQ=255)

                                   cGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAagg                                                                                                 >  1:14476/1‑138 (MQ=255)

                                    ggCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggga                                                                                                   >  1:132080/1‑133 (MQ=255)

                                       tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGTCGGGGCAAGA‑Ggaa                                                                                            >  2:124377/1‑139 (MQ=255)

                                       tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga‑gga                                                                                             >  1:234404/1‑136 (MQ=255)

                                        cccGTAATTCATTCCCCCCACCACCCGCGCATTAACCCACCCCAATGTGAAAAAA‑TG‑CCTC‑G‑GGGGCGGCT‑TCCGCCA‑GGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAAt                                                                                           <  2:263858/138‑1 (MQ=255)

                                         cctttaTTCTTCTCCACAAACACCCGCTCATTTCACCAACGCTATTTGAAAAAT‑TG‑CATT‑C‑GCGTGGTTTGTTTGCAA‑CCTTTTTTT‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAAt                                                                                           <  1:365145/133‑1 (MQ=255)

                                                ttaTTCCCGCCAACACCCGCGCATTTACCCACCGCCTTGTGAAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑a                                                                                  <  1:706667/136‑1 (MQ=255)

                                                 cATTCCCGCCAACACCCGCGCTTTTATCCAACGCCTTGTGCAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCTGCCA‑GTTTTTTTT‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑aa                                                                                 <  1:862252/138‑1 (MQ=255)

                                                  aTTCCCCCCAACACCCGCGCATTTACCCACCCCAATGTGCAAAAA‑TG‑CCTC‑C‑GCGGCGGCTTTCTGCCA‑GCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGc                                                                               <  1:878993/139‑1 (MQ=255)

                                                    tccccccaccaCCCGCGCATTTACCCACCGCAATTTGAAAAAA‑GG‑CCTC‑C‑GCGGCGGCTGTCTCCCA‑GCTATTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGc                                                                               <  1:888797/132‑1 (MQ=255)

                                                    tccccccaccaCCCGCGCATTTACCCAACGCCTTGTAAAAAAA‑TC‑CCTC‑C‑GCGGCGGCTGTCTCCCA‑TTTTTTTTC‑CCCCCACAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGc                                                                               <  2:419988/132‑1 (MQ=255)

                                                     cccGCCAACACCCGCGCATTTACCCACCGCCATGTGAAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGccc                                                                             <  1:732151/137‑1 (MQ=37)

                                                      cacccAACACCCGCGTATTAATCCAAGGTTATGCGAAAAAA‑TG‑TTTC‑G‑GGGGCGGGTGGCTGCCG‑GTTTTTTTG‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCg                                                                            <  2:185411/135‑1 (MQ=255)

                                                       ccccAACACCCGGCCTTTCACCCACCGCCATGTGCAAAAA‑TC‑CCTT‑C‑GCGGCGGTTGTCTCCCA‑GGTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTc                                                                          <  2:66617/137‑1 (MQ=255)

                                                       ccccAACACCCGCTCTTTTACCCAACGCCATGTGCAAAAA‑GG‑CCTT‑C‑GCGGGGGTTGTCTGCCA‑GTTTTTTTC‑CCGCCACACAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTc                                                                          <  1:477825/137‑1 (MQ=37)

                                                       ccccAAC‑ACCGCCCATTTTACCCACCCCAAGGGAAAAAATTC‑CCTC‑C‑GCGGGGGCTTTCTGCCA‑TTTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTc                                                                          <  2:5649/137‑1 (MQ=255)

                                                       cccaaacaccCGCCCATTTA‑CCAACCCCCGGGGCAAAAA‑AGCCCTC‑C‑GCGGGGGCTGTCGGCAA‑GCTTTTTTC‑CCGCCGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTc                                                                          <  2:213316/135‑1 (MQ=255)

                                                                   cgcATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGCGA‑GAAAAAAA‑ATT‑AAACCCCCCCTGTTTTGTggg                                                              >  2:212606/1‑138 (MQ=25)

                                                                       tttACCCAACGCCATGTGCAAAAA‑TG‑CTTC‑C‑GCGGCGGCTGTCTGCCA‑GTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAAt                                                           <  1:645086/138‑1 (MQ=39)

                                                                        agacccAACGCCATGTGAAAAAA‑TG‑CCTC‑C‑GGGGGGGTTGTCTGCCA‑GCTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  1:825148/135‑1 (MQ=255)

                                                                         tacccAACGCCTTGTGAAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑TCTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  2:358011/135‑1 (MQ=255)

                                                                         tacccAACGCCATGTGAAAAAA‑TG‑CTTC‑C‑GCGGCGGCTGTCTGCCA‑GCTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  1:212606/135‑1 (MQ=255)

                                                                         tacccAACGCCAGTGGAAAAAA‑CG‑CCTC‑G‑GCGGCGGCTTTCCGCCG‑GTTTTTTTC‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  2:184641/135‑1 (MQ=255)

                                                                         tATCCACCCCCATGTGAAAAAA‑TG‑CTTG‑C‑GCGGCGGTTGTCGGCCA‑GTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  1:87925/138‑1 (MQ=255)

                                                                         tATCCAACCCCAGGGAAAAAAA‑TC‑CCTC‑C‑GCGGCGCCTGCCTGCCA‑GTTTTTTTC‑CCGCCGAAACAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCg                                                         <  2:392763/138‑1 (MQ=255)

                                                                           tCCACCCCCATGTCAAAAAA‑GG‑CCTC‑C‑GCGGCGGTTGTCTGCAA‑GTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCgct                                                       <  2:588983/138‑1 (MQ=255)

                                                                           cccAACGCCATGTGAAAAAA‑TG‑CCTC‑C‑GGGGCGGCTGTCTGCCA‑GTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCgct                                                       <  1:728752/137‑1 (MQ=255)

                                                                           cccAACGCCATGTCAAAAAA‑GC‑CCTC‑C‑GCGGCGCCTTCCTGCCA‑TCTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCgct                                                       <  2:372454/137‑1 (MQ=255)

                                                                           cccAACCCCATGTGCAAAAA‑TC‑CTTC‑C‑GCGGCGGTTCCCTGCCA‑GCTTTTTTC‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCgct                                                       <  2:346330/137‑1 (MQ=255)

                                                                             cacccccATGTGCAAAAA‑TG‑CCTC‑C‑GGGGCGGCTGTCTGCCA‑GCTATTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCgctg‑ct                                                   <  1:270431/134‑1 (MQ=255)

                                                                                cGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGGAAAAAAA‑TTT‑AA‑ACCGCCCTGTTGTGGGGGGATTGCTG‑CTggg                                                >  2:728752/1‑138 (MQ=21)

                                                                                cGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGA‑GAAAAAGC‑ATT‑TAACCCCCAACGTTGTGGGGGAAACGCTG‑CTGGt                                                >  1:346330/1‑139 (MQ=25)

                                                                                cGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAAAAAC‑ATT‑TAACCCCCCATGTTTTGTGGCGATCGCCG‑CTGGt                                                >  1:69693/1‑139 (MQ=37)

                                                                                cGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAAA‑GAAAAAGA‑ATT‑TAAACCGCCATGTTTTGTTGGGAACGCTG‑CTggg                                                >  1:521362/1‑138 (MQ=25)

                                                                                cGCCATGTGAAAAAA‑TG‑CCTT‑C‑GGGGCGGCTGTCTGCCA‑GTTTTTTTC‑CCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGt                                                <  1:600447/139‑1 (MQ=255)

                                                                                cGCCATGT‑CAAAAA‑CT‑CCTTCC‑GCGGCTCTGGTCTCCCT‑TCTTTTTTC‑CCCCCTCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGt                                                <  2:833027/139‑1 (MQ=255)

                                                                                 cccATGTACAAAAA‑CC‑CCCC‑C‑GCGGCGCCTTCCTGCCA‑TTTTTTTTC‑CCGCCGCAACAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTa                                               <  2:74194/138‑1 (MQ=255)

                                                                                  ccATGTACAAAAA‑TG‑CTTC‑C‑GGGCCGGTTGTCTGCCG‑TTTTTTTTC‑CCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTaa                                              <  2:598470/139‑1 (MQ=255)

                                                                                    aTGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑GAAAAAACTTTT‑AACCCCCCCTTGTT‑TGTGGGAAACGCCG‑CTGGtgaa                                             >  1:784647/1‑135 (MQ=25)

                                                                                    aTGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGA‑GAAAAAAC‑TTT‑TATCCCCCCCTGTTTTTTGGCAATCGCTGCCTGGTac                                              >  1:93629/1‑137 (MQ=25)

                                                                                     tgtgCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAAAAGA‑TTT‑TAAACCCTCCAGTTGTGTGGGAATAGCCG‑CTggggaca                                            >  1:762960/1‑133 (MQ=25)

                                                                                     tgtgCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAGA‑GGAATAAA‑TTT‑TAAACCCCCCTGGTGTGTGGGCATCGCCG‑CCGGGAACa                                            >  1:129265/1‑138 (MQ=25)

                                                                                     tgtgCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGC‑AAAAAAAG‑TTT‑ATACACCGCCTGTTTTTTGGCTATCGCCT‑CTGGTaaaca                                           >  1:562926/1‑136 (MQ=25)

                                                                                       tgaaaaaa‑TG‑CCTG‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAAt                                          <  1:491229/135‑1 (MQ=255)

                                                                                       tgaaaaaa‑TC‑CCTC‑C‑GCGGGGCCTTTCTCCCA‑CTTATTTTC‑CCCCCACAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAAt                                          <  1:187367/135‑1 (MQ=255)

                                                                                       tccAAAAA‑TG‑CCTC‑C‑GCGGCGGCTGTCTGCCG‑GTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAAt                                          <  2:159464/136‑1 (MQ=255)

                                                                                           aaaa‑TG‑CCTC‑G‑GGGGGGCCTGTC‑GCCAGGTTATTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGa                                     <  2:118416/139‑1 (MQ=255)

                                                                                                 g‑CCGT‑C‑GGGGCGGCTGTCCGCCG‑TTTTTTTCC‑CCGGCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAggg                                <  2:326039/139‑1 (MQ=255)

                                                                                                   ccTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGGAAAAAAT‑TTT‑AAACCC‑CCATGTTTTGTGGGGATCGCTG‑CTGGGGAAAAAGTGGGAAggg                                >  1:244733/1‑138 (MQ=255)

                                                                                                   ccTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGACA‑GAAAAAGC‑TTT‑AAAAACGCCTTGTTTTGTGGCGCAAGCTG‑CTGGTTACCAAGTggggaagg                                >  1:617512/1‑132 (MQ=255)

                                                                                                      t‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAAA‑GGAAAAAC‑TTTAAAGCCCG‑CCTGTTGTGTGGGAAATGCTG‑CTGGGAAAAAAGTGGGAAGGGGAgg                            >  1:378930/1‑138 (MQ=255)

                                                                                                                   tccTGCCA‑TCTATTTTC‑CCCCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  1:483626/136‑1 (MQ=255)

                                                                                                                    tccgCCA‑GCTTTTTTC‑CCGCCGAAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  2:132080/134‑1 (MQ=255)

                                                                                                                    tCTGCCA‑TTTTTTTCC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  2:374387/137‑1 (MQ=255)

                                                                                                                    tCTGCCA‑GTTTTTTTT‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  1:254734/137‑1 (MQ=255)

                                                                                                                    tCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  1:713731/137‑1 (MQ=255)

                                                                                                                    tCTGCCA‑CTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  1:168935/137‑1 (MQ=255)

                                                                                                                    ccggCCA‑GTTATTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                  <  1:402918/134‑1 (MQ=255)

                                                                                                                          cacttATTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa           <  1:423214/135‑1 (MQ=255)

                                                                                                                            gCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GGAAAAAC‑TTT‑TAACCCCTCCTGTTGTGTGGGGATCCCCG‑CTGGTGAAAAAATGGGAAGGGGAGTGGGGGGGAAGAGCTcccg          >  1:587031/1‑136 (MQ=255)

                                                                                                                              atttttc‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc       <  2:206608/137‑1 (MQ=255)

                                                                                                                               aTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc       <  1:344678/138‑1 (MQ=255)

                                                                                                                                ttttt‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc       <  1:795443/137‑1 (MQ=255)

                                                                                                                                ttttc‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc       <  1:18706/137‑1 (MQ=255)

                                                                                                                                 tttc‑CCCCCGCAACAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcg     <  1:911084/138‑1 (MQ=255)

                                                                                                                                    tcccgccaAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgcg   <  1:831156/136‑1 (MQ=255)

                                                                                                                                    t‑CCCCCCCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCgcgc  <  1:411854/138‑1 (MQ=255)

                                                                                                                                    ggccgcagCAAAAGACCCGCCGTTCTCGCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgcg   <  2:61228/136‑1 (MQ=255)

CGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TG‑CCTT‑C‑GCGGCGGCTGTCTGCCA‑GCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTT‑AAGCCCGTCATGTTGTGTGGCAATCGCTG‑CTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGC  >  NZ_CP009273/353464‑353728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`