Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273529,7870TG52.4% 17.5 / 32.3 21V139G (GTG→GGG) gclglyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base T (3/7);  new base G (11/0);  total (14/7)
Fisher's exact test for biased strand distribution p-value = 1.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-04
Rejected as consensus: Frequency below/above cutoff threshold.

TTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGT‑CCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCG  >  NZ_CP009273/529655‑529917
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ttccTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTACAGTTCGTGAAGCGGCGCTGGTGCCTcg                                                                                                                               <  2:356073/139‑1 (MQ=255)
           tgCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTTTATTTCTTTAAGAGGTGCCTGTGCCCCCCCTGCTGCaa                                                                                                                     >  1:742313/1‑138 (MQ=255)
           tgCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑GTACAGTTTCTGAAGCGGGGCTGGGGGCTCCCCtgcgccca                                                                                                                     >  1:437992/1‑133 (MQ=255)
                                cgcgcCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTACAATTCGGGGAAGGGCGCGGGGGGCCCGCCGGCTGCCACAAGCATTTTCCCTTGTGCGtt                                                                                               >  2:856623/1‑139 (MQ=255)
                                                  gaagaTTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCgt‑ccgggtccgg                                                                            <  2:18183/139‑1 (MQ=255)
                                                      aTTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTTCCGTTTGTGAAACGGCGCTTGGGCGCCTCGGGGTGCCACAAGGATTTTTCCTGATGCGGTTTGGGGGT‑CCGGGGCCGGGACt                                                                        >  2:121636/1‑139 (MQ=255)
                                                      aTTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTTAAGTTTGTGGAGCGGCGCTGGGGGATCGCGGGCCGCAACAGGCAATTCACCTGCTGGGTTTTTGTCGT‑CCGGGGTCGGtgag                                                                        >  1:440169/1‑136 (MQ=255)
                                                                cGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGGTTTAAAGTT‑GTGAAGAGGGGCTGGGGGCCCGCGTGCTGCAACAGGCCTTTCACCTTATGCGTTTTGGTCGT‑TCGGGGCCGGGACTGGGGGGTTTa                                                              >  2:731248/1‑139 (MQ=255)
                                                                      tatTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGT‑CCGGGTCCGGTACTGGTGGATTTACCGTTc                                                        <  2:749015/139‑1 (MQ=255)
                                                                      gatTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGT‑CCGGGTCCGGTACTGGTGGATTTACCGTTc                                                        <  2:15074/138‑1 (MQ=255)
                                                                         tGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTACAATTCGGGGAGGGGGGCCGGGGGCCCCCGGGCTGCAAAAAGCATTTTTCCTGATGCGGTTTGGGCGG‑CCGGGGCCGGGGATGGGGGGTTTTCCGtttggc                                                     >  1:436960/1‑135 (MQ=255)
                                                                                aTTGCTAAACCGGTCAGCAAAATGGCGG‑TTACAATTCGTGAAGCGGCGCTGGGGCCTCCCGTGCTGCCACAGGCATTTTACCTGATGCGTTCTGGGCGT‑CCGGGTCCGGGACTGGGGGGTTTACCGTTCGACGTTCAgg                                              >  1:42978/1‑139 (MQ=255)
                                                                                            gTCAGCAAAATGGCGG‑TTTCAGTTTGCGAACGGGCTATGGGGGCTCGCGTGCCGGAAAAGGCATTTTACCCCATGCGTGCTTGTTGTGCCGGGCCCCG‑ACTGGTGGAATTTTCGTTCTACGGTCTGGGTGGGGgaaat                                   >  1:40617/1‑136 (MQ=255)
                                                                                            gTCAGCAAAATGGCGG‑TTTACGTTCGTGAAGCGGGGCTGGGGGCCCGCGTGGTGCAAAAGGCATTTCTCCTGATGCGTTCTGGTTGT‑CCGGGGCCGGGACTGGGGGGTTTACCGTTTGGCGTTCAGGGTGCGGGAAACg                                  >  1:714825/1‑139 (MQ=255)
                                                                                            gTCAGCAAAATGGCGG‑TTACAGTTTGTGAAGCGGCGCTGGGGCCTCGCGGGCTGCAAAAAGCCTTTTCCCCGATGCGGTCTGGTCGT‑CCGGGGGCGGGACTGGGGGGTTTTCCGTTCGGCGGTTAGGGTGCGGaaaaag                                  >  1:766025/1‑136 (MQ=255)
                                                                                            gTCAGCAAAATGGCGG‑TTACAGTTCGTGAAGCGGCGCTGGGGGCTCGCGTGCTGCAACAGGCATTTTTCCCGATGCGGTCTGGGCGT‑CCGGGGCCGGGACTGGGGGGTTTACCGTTCGACGTTCAGGGTGCGGGAATCg                                  >  1:197714/1‑139 (MQ=255)
                                                                                                                cAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGT‑CCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTa               <  1:856623/139‑1 (MQ=255)
                                                                                                                           gcggcgCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGT‑CCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGc    <  2:197714/139‑1 (MQ=255)
                                                                                                                             ggggcTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGT‑CCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCg  <  2:98493/136‑1 (MQ=255)
                                                                                                                             ggcgCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGT‑CCGGGTCCGGTACTGGTGGATTTATCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTAGGAACCGCAGCCg  >  2:287308/1‑139 (MQ=255)
                                                                                                                             ggcgCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGT‑CCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCg  >  2:100945/1‑139 (MQ=255)
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TTCCTATTCTGTGCATTACCGGCCAGGCACCGCGCGCCCGTCTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGG‑TTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGT‑CCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCG  >  NZ_CP009273/529655‑529917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑