| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 982,950 | 0 | T | G | 62.5% | 6.0 / 16.3 | 17 | intergenic (‑512/+91) | ompF/asnS | porin OmpF/asparagine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (10/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGG > NZ_CP009273/982823‑983071 | cAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGaaataaat > 2:786185/1‑139 (MQ=255) gtgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTGCTTTACTTaacaaaaaa > 2:111014/1‑135 (MQ=255) tAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTGTTTGAATTTACACAAACATAAAtttata > 2:320241/1‑134 (MQ=255) aTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGCTTCTCATAGATTTCTTTACTTAACCAAAAGAAAAATTTAGAGATCAGATaaaaa > 1:5709/1‑138 (MQ=255) cGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAATTTTTTTACTTTAAAAAAAAAAAAATTTACAGAAAAAAAAAAAAAAAAAACAGCGGGGGCt > 1:856965/1‑139 (MQ=255) cGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGGGCTAATAGATATATTTGTTTAACAAAAAAAAAACATGAAGGAAAAGATAAAAAAAAAACCCGCGGGGGCt > 2:372270/1‑139 (MQ=255) ttGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGaa < 1:797288/138‑1 (MQ=255) cgcgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACg < 1:111014/139‑1 (MQ=255) cAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGc < 1:786185/139‑1 (MQ=255) ttCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTAcg < 1:795199/139‑1 (MQ=255) cGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTAcgcg < 1:348728/138‑1 (MQ=255) cGTAACAGGATGATCGTTATGGCGGGGGTTCAAATAAATTTCTTTACTTTAAAAAAAAATAACTTTAAAGAAAAGAAAAAAAAAAAGCCCGCGGGGGCCGGGGCTTGAAGACGCCAAAATAGAAACCGGCGGTAcgcg > 2:41370/1‑138 (MQ=255) tAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATTCTTGAATTAAAAAAAAAATAAATTTACAGAAAAAATAAAAAAAAAAACAGCGGGGGCTGGCTCTTTAAGACGCCAAATTAAAAAATGGCGGTTCGCgggg > 2:348728/1‑137 (MQ=255) tAACAGGATGATCGTTATGGCGGGGGTGCAAATAGATTGCTTGACTTTACAAAAAGAAAACTTGACAGAAAAGATAAAAAAAAAGCCCGCGGGGGCTGGCCCTTGTAGAAGCCAAAATAGAAACTGGCGGTACGCGGAg > 1:215860/1‑139 (MQ=255) tAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTTCTTGTCTTTAAAAAAAGAAAAAATGACAGAAAAAAAAAAAAAAAAAACAACGGGCGGCGGGTCTTTTAAAAGGTAAATTAGAAACTGGCGGTAAGCgggg > 2:795199/1‑137 (MQ=255) gatgatCGTTATGGCGGGGGTGGAAATAAATTTTTTTACTTTAAAAACAGAAAAATTGAAAGAAAAGATAAAAAAAAAACCCACGGGCGCCGGCTCTTTGAGACGCTAAATTAAAAGCTGGGGTTACGCCGAGTACggg > 2:486872/1‑137 (MQ=255) tgatCGTTATTGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTggg < 1:372270/139‑1 (MQ=255) | CAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGG > NZ_CP009273/982823‑983071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |