| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,129,313 | 0 | A | G | 53.3% | 2.8 / 12.2 | 15 | Q19R (CAG→CGG) | flgF | flagellar basal‑body rod protein FlgF |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base G (0/8); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCAACGCC > NZ_CP009273/1129184‑1129442 | ccAGACCACAAAACCCCAGGACCAGATCCTCAACCCGCTGGTTAACTTCCGCTAATCGCTGAGGGGAAAGCTAAAGGGATCACGAAATTTATCCCGCGAGGGGAGCACCCAGCCAGACACTAAATCAACGGGCGGTAAc < 2:333465/139‑1 (MQ=255) aGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCg > 1:358173/1‑139 (MQ=255) cAGCACCAGATCCTCAACCCGCTGTTTAACTTCCGCTAACCGCGGCGGGAATACCAAAATGGATCCCGAAATTGATCCCGCGAGGGGAGCACCCACCCAGACACTAAAACAACAGGCGGTAACCGCCAGTAATCTGGcc < 2:787672/139‑1 (MQ=255) aaCACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGccc > 1:208793/1‑139 (MQ=255) cTAACCGCTGACGGGATAGCTAAGTGGACCACGAATTTTACCCCGCGGGGGGAGCAGCCAGCCAGCCCCTGAACCAACGGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGaa < 1:516449/139‑1 (MQ=255) aTCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGc > 2:692394/1‑139 (MQ=255) cggggATAGCTAAATGGACCCCGCAATTTATCCCGCGATGGGAGCAGCCACCCAGACACTAAAACAACGGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTAcg < 1:471955/136‑1 (MQ=255) ggggATGCCTAAAGGGACCACGAAATTTATCCCGCGAGGGGAGCACCCAGCCAGACCCTGAATCACCGGGGGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTAcgcg < 1:505895/138‑1 (MQ=255) cACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGc > 1:275051/1‑139 (MQ=255) gaaaTTTATACCGCGAGGGGAGCAGCCACCCAGACACTGAATCACCAGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCttt < 1:150134/137‑1 (MQ=255) aaTTTATCCCGGGATGGGAGCACCCAGCCAGACATTAAATCAACAGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTtct < 1:210681/139‑1 (MQ=255) ggcgAGGGGAGCACCCAGCCAGACACTGAATAAACGGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCAcg < 1:204301/138‑1 (MQ=255) gggACCAGCCAGCCAGACACTGAATCAACGGGGGTTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACg < 2:208793/139‑1 (MQ=255) gggACCAGCCAGCAAGACACTGAACCAACGGGCGGAAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACg < 1:518581/139‑1 (MQ=255) aaTCAACGGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCAACGcc < 1:597787/137‑1 (MQ=255) | CCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTAATCTGGCCAATGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCAACGCC > NZ_CP009273/1129184‑1129442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |