| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,574,621 | 0 | A | G | 53.8% | 1.6 / 13.8 | 13 | K543E (AAA→GAA) | tkt | transketolase |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (7/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGGGCTGGAAGCTGGCGGTTGAGCGCCACAACGGACCGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGAAAGACAGCGGCGGTAAGCCAGATA‑TTATTCTGATTGCCACCGGTTCAGAGAT‑GGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCG > NZ_CP009273/2574491‑2574759 | ggTGGGCTGGAAGCTGGCGGTTGAGCGCCACAACGGACCGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGAAAGACAgc < 1:92288/139‑1 (MQ=255) tGGCGGTTGAGCGCCACAACGGACCGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGGGGTGGAAGACAGCGGCGGGAAgaccg > 1:692470/1‑134 (MQ=255) cGGTTGAGCGCCACAACGGACCGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGGAAGACAGCGGCGGGAAAACAGata‑t > 1:406023/1‑139 (MQ=255) aCGGACCGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGAAAGACAGCGGCGGTAAGCCAGATA‑TTATTCTGATTGCCAc < 1:647004/139‑1 (MQ=255) cGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTTCTGGAAGAAAGCGGCGGGGAAGCAGATATTTTTTTTGTTTGCCCCCGGtt > 2:848054/1‑139 (MQ=255) ctcAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGGAAGAAAGCGGGGGTAAGGCAGATA‑TTTTTTTTATTGCTCCCGGGTCAGACATGGGAAAttcc > 2:26066/1‑137 (MQ=255) gTGGAACGTACGCCGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGGAAGACAGCGGCGGGGAGCCAGATATTTTTTTTTTTTCCCCCCGGTTC‑GAGAT‑GGAAAATACCCTGCAAGCGGCAGAGAAAttta > 1:89423/1‑137 (MQ=255) cGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGAAAGACAGCGGCGGTAAGCCAGATA‑TTATTCTGATTGCCACCGGTTCAGAGAT‑GGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCg < 2:374399/139‑1 (MQ=255) gATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGAAAGACAACGGCGGGAAGCCAAATA‑TTTTTCTGTTTTCCACCGGGTCAGAGAT‑GGAAATTAACCCTCAAACGGCAGAGGAATTAACAGGGAAAGGGCgg > 2:248865/1‑137 (MQ=255) aGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGAAAGACAGCGGCGGTAAGCCAGATA‑TTATTCTGATTGCCACCGGTTCAGAGAT‑GGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATg < 1:848054/138‑1 (MQ=255) agagATTGCTCGTGGCGGTTATGTGCTGGAAGACAGCGGCGGGAAAGCAGATA‑TTTTTTTTATTGGCCCCGGGTTCGAGGA‑GGGAAATAACCTGCAAAAGGGAGAGAAAATAGCAGGGGAAGGGGGCAAAGGAcgcg > 1:439731/1‑137 (MQ=255) ttGCTCGTGGCGGTTATGTGCTGGAAAACAGCGGGGGGAAGGCCGATA‑TTTTTTTGATTGCCCCCCGGTTAGAGAT‑GGGAAATACCCCGCAAACGGCAGGGGAAATAACAGGGGAAAGGGGGAATGTACGCGGAGtttt > 2:521093/1‑138 (MQ=255) ttGCTCGTGGCGGTTATGTGCTGAAAGAAACCGGCGGTAAGCCAGATA‑TTATTCTGATTTTCACCGGGTCAGAGAA‑GGAAAATACACCGCCAACGGCCGAGAAATAAGAAGGGGAAAGGCGCAAAGTAAGCGGAGgttt > 2:634978/1‑138 (MQ=255) aaaGACAGCGGCGGTAAGCCAGATA‑TTATTCTGATTGCCACCGGTTCAGAGAT‑GGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCg < 2:314079/139‑1 (MQ=255) | GGTGGGCTGGAAGCTGGCGGTTGAGCGCCACAACGGACCGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAGAGATTGCTCGTGGCGGTTATGTGCTGAAAGACAGCGGCGGTAAGCCAGATA‑TTATTCTGATTGCCACCGGTTCAGAGAT‑GGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCG > NZ_CP009273/2574491‑2574759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |