Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NZ_CP009273	1,193,954	(AATGATGA)6→5	intergenic (‑261/‑190)	BW25113_RS05920 ← / → lit	hypothetical protein/cell death peptidase Lit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP009273	1193918 =	1 (0.020)	26 (0.830)	19/158	0.4	96.9%	intergenic (‑225/‑233)	BW25113_RS05920/lit	hypothetical protein/cell death peptidase Lit
	?	NZ_CP009273	= 1193909	1 (0.030)					intergenic (‑216/‑242)	BW25113_RS05920/lit	hypothetical protein/cell death peptidase Lit

TTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCT  >  NZ_CP009273/1193775‑1194041                                                                                                                                       |                                                                                                                                     tttGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatg                                                                                                                                   >  2:597933‑M2/1‑135 (MQ=255)           ttCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaa                                                                                                                        <  1:902846‑M2/139‑15 (MQ=255)                gCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgat                                                                                                                    <  2:680186‑M2/138‑19 (MQ=255)                   gACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaa                                                                                                                <  2:301303‑M2/139‑23 (MQ=255)                               cTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgat                                                                                                    <  2:841642‑M2/139‑35 (MQ=255)                                          tatTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgac                                                                                         >  2:618517‑M2/1‑94 (MQ=255)                                                 aTCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatga                                                                                  >  1:869038/1‑139 (MQ=255)                                                        gCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtg                                                                            >  1:105454‑M2/1‑80 (MQ=255)                                                              aTCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacg                                                                      <  2:549638‑M2/138‑65 (MQ=255)                                                               tCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacgga                                                                    >  1:600811‑M2/1‑73 (MQ=255)                                                                 gaggaggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatg                                                                  >  1:616709‑M2/1‑71 (MQ=255)                                                                 gaggaggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatg                                                                  >  1:680186‑M2/1‑71 (MQ=255)                                                                      ggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtc                                                             <  2:474198‑M2/139‑74 (MQ=255)                                                                      ggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtc                                                             >  2:440567‑M2/1‑66 (MQ=255)                                                                        agTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatg                                                                  >  2:499300‑M2/1‑64 (MQ=255)                                                                        agTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatg                                                                  <  1:499300‑M2/132‑69 (MQ=255)                                                                        agTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtcc                                                                                <  2:575079‑M2/118‑55 (MQ=255)                                                                        agTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtcc                                                                                >  1:575079‑M2/1‑64 (MQ=255)                                                                            gcgcTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacga                                                       >  1:598645‑M2/1‑60 (MQ=255)                                                                             cgcTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgac                                                      >  1:449541‑M2/1‑59 (MQ=255)                                                                                                     gaATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagt                              >  1:549638‑M2/1‑35 (MQ=255)                                                                                                     gaATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagt                              >  1:166880‑M2/1‑35 (MQ=255)                                                                                                     gaATCTGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagt                              >  1:739046‑M2/1‑35 (MQ=255)                                                                                                          tGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggca                                                                        >  1:744095‑M2/1‑30 (MQ=255)                                                                                                          tGAAATTGATAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggca                                                                        <  2:744095‑M2/92‑63 (MQ=255)                                                                                                                  aTAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcag                 >  2:902846‑M2/1‑22 (MQ=255)                                                                                                                  aTAGAAATGAAATAATGAAATAatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcag                 >  1:474198‑M2/1‑22 (MQ=255)                                                                                                                             ataatgaaataatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaa       <  1:712694‑M2/138‑128 (MQ=255)                                                                                                                                atgaaataatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatg                                                                  <  2:136245‑M2/76‑69 (MQ=255)                                                                                                                                atgaaataatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatg                                                                  >  1:136245‑M2/1‑8 (MQ=255)                                                                                                                                 tgaaataatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaacatgt  <  2:709348‑M2/139‑133 (MQ=255)                                                                                                                                 tgaaataatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgacagagtgtccagtgggcacggatggtgtcttacgacatgcttaccttaatcgttcccagtgtgcctatagcagatattctaaacatgt  >  1:708548‑M2/1‑7 (MQ=255)                                                                                                                                       |                                                                                                                                     TTTGTATCTGTTCAGGCTGACCTAGCTTCGCTGACAGACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCT  >  NZ_CP009273/1193775‑1194041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.