Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273228,1810CT35.9% 52.9 / 10.1 40F191F (TTC→TTT) yafDendonuclease/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base C (9/16);  new base T (14/0);  total (24/16)
Fisher's exact test for biased strand distribution p-value = 8.56e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGT  >  NZ_CP009273/228044‑228316
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gACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGCTGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGCGGTTTc                                                                                                                                          >  2:576306/1‑138 (MQ=255)
 acacCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCa                                                                                                                                         <  1:853311/138‑1 (MQ=255)
     ccGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGAGTTCAATggc                                                                                                                                    >  1:342305/1‑137 (MQ=255)
                tggtggTTAATATACACGCCGTCAACTGCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTACAATGCCTGGAGCCGTA‑g                                                                                                                        <  1:28215/139‑1 (MQ=255)
                 ggtggtTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGTCTgg                                                                                                                                 >  2:13364/1‑130 (MQ=255)
                 ggtggtTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTgg                                                                                                                                 <  1:13364/130‑1 (MQ=255)
                   tggtTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAAAGCCCGGGGCCGGG‑GAAg                                                                                                                     >  1:185724/1‑139 (MQ=255)
                      ttAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGAt                                                                                                                  >  1:337027/1‑139 (MQ=255)
                        aaTATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATTGCTGGAg                                                                                                                               >  1:467811/1‑125 (MQ=255)
                        aaTATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAg                                                                                                                               <  2:467811/125‑1 (MQ=255)
                         atatACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGaa                                                                                                               <  2:312478/139‑1 (MQ=255)
                         atatACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGa                                                                                                                <  1:468670/138‑1 (MQ=255)
                               acGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGGTTTAATGACCGGGGCCGTAGGAAGGAGGaagcggt                                                                                                          >  2:753266/1‑134 (MQ=255)
                               acGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTCAATTCCTTGAGCCGTA‑GAAGGATGAAcgcggt                                                                                                         >  1:27781/1‑137 (MQ=255)
                               acGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGGTTTTAATTCCTGGAGCCGTA‑GAAGGGTGAACGCGtt                                                                                                         >  2:442248/1‑139 (MQ=255)
                                    gTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGGTTTTAATTCCTGGAGGCGGA‑GGAGGATGAACGCGGTATATc                                                                                                    >  2:135244/1‑139 (MQ=255)
                                    gTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGGTTTTAATGCCTGGAGCCGGA‑GGAGGATGGACGCGGTttatt                                                                                                    >  2:16042/1‑138 (MQ=255)
                                     tCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCCATGCCTGGGGCCGCA‑GAAGGGTGAAAGCGGTATATCg                                                                                                   >  1:330336/1‑139 (MQ=255)
                                       aaCTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCt                                                                                                 <  1:16042/139‑1 (MQ=255)
                                                             tCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTTAATGCCTGGGGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGGAATGTCGCTgcg                                                                           >  1:849063/1‑139 (MQ=255)
                                                                 taGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAg                                                                       <  2:217409/139‑1 (MQ=255)
                                                                 taGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAg                                                                       <  1:442248/139‑1 (MQ=255)
                                                                      aGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTTAATGCCTGGAGCCGGA‑GAAGGGTGAACGCGTTATATCGCTTTGTGCGGGGAATGTCGCGGCGCCAGGTGCg                                                                  >  2:564774/1‑139 (MQ=255)
                                                                      aGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTTAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTTCGCGGGGAATGTCGCTGCGCCAGGTGCg                                                                  >  1:192395/1‑139 (MQ=255)
                                                                      aGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATAATGCTTTGCGCGGGGAAGGTCGCTGCGCCCGGGGCg                                                                  >  2:400450/1‑139 (MQ=255)
                                                                      aGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTATATGCCTGGGGACGCA‑GAAGGAGGGACGAGGTATATTGCTTTTCGCGGGGAAACTGGCCGCGGCCGGTGCg                                                                  >  2:617666/1‑139 (MQ=255)
                                                                             aCTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGGTTTTAATGCCTGGAGCCGTT‑GAAGGAAGAACGCGTTATATTCCTTTTCGCGGGGAAAGTCGGTGCGCCAGGGGGGGTTTAcc                                                           >  1:503644/1‑139 (MQ=255)
                                                                              cTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCg                                                          <  1:509879/139‑1 (MQ=255)
                                                                                        gCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAgcg                                                <  1:412369/139‑1 (MQ=255)
                                                                                           aTCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAAAGCCCGGGGCCGGA‑GAAAGATGACCGCGCTTTATTGCTTTTCGCGGGGAATGTCGCTGCGCCCGGGGCGGTTTTTCGATTATACGCGCCg                                             >  2:714127/1‑139 (MQ=255)
                                                                                           aTCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGAATTTAAAGGCCGGAGGCGGA‑GAAGGATGAAAAGGGTATATTGCCTTGTGCGGGGAATGTCGCGGCGCCAGGGGCGTTTTAACGATGATTAGCGCCg                                             >  1:226140/1‑139 (MQ=255)
                                                                                             cAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTTAATGCCCGGAGCCGTA‑GAAAGATGGACGCGTTATATCGCTTTGCGCGGGGAAAGTCGCTGCGCCAGGGGCGTTTTATCCATTATAAGCGCCggc                                           >  2:803874/1‑137 (MQ=255)
                                                                                                 tAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGc                                       <  1:803874/139‑1 (MQ=255)
                                                                                                          acaGCGGCCCGGTCATTATGGCGGGAGGTTTTAAAGCCTGGAGCCGTA‑GAAGGAGGAAAGCGTTATATTGCTTTGCGCGGGGAAAGTCGCTGCGCCAGGTGGGGTTTTCCGGTGATCAGCGCCGTCGGGCGTTTTGTCg                              >  1:471490/1‑139 (MQ=255)
                                                                                                          acaGCGGCCCGGTCATTATGGCGGGAGATTTCAAAGCCTGGAGCCGTA‑GAAGGATGAACGCGGTATATCGCTTTGCGCGGGGAATTTCGCCGCGCCAGGGGCGTTTTACCGGTGATCAGCGCCGTCGGGCGTTTGGTCg                              >  1:943111/1‑139 (MQ=255)
                                                                                                                gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCt                        <  2:185724/139‑1 (MQ=255)
                                                                                                                gCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCt                        <  1:853639/139‑1 (MQ=255)
                                                                                                                   cGGTCATTATGGCGGGAGACTTCAATGCCTTGGGCCGGG‑GGAGGATGAACGCCGTGTATAGCTTCTCGCCGGAAAATGCGCCGCGCCCGGGCGGTTTTTCCGGTGATCAGCGCCGTTAGGCGTTTGGTGGCCCGCTCGa                     >  2:858460/1‑139 (MQ=255)
                                                                                                                     gTCATTATGGCGGGAGATTTTAATGCCTGGAGGCGTA‑GAAGGATGGAAGCGGTATATTGCTTTGCGCGGGGAATGTCGCTGCGCCAGGGGCGGTTTACCGGTGATCAGCGCCGTCGGGGGGTTGGGGGCCCGCCCGGtt                   >  1:163345/1‑139 (MQ=255)
                                                                                                                                  gagaTTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCg      <  1:135244/139‑1 (MQ=255)
                                                                                                                                      tttCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGt  <  2:909603/139‑1 (MQ=255)
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GACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTA‑GAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGT  >  NZ_CP009273/228044‑228316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑