Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273353,5910TC50.0% 61.4 / 29.5 57K271E (AAA→GAA) cynRtranscriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base T (24/4);  new base C (0/28);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 9.40e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAC  >  NZ_CP009273/353453‑353723
                                                                                                                                           |                                                                                                                                     
gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTAttttt                                                                                                                                       >  1:289586/1‑139 (MQ=255)
gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTAttttt                                                                                                                                       >  1:627512/1‑139 (MQ=255)
  tAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTcc                                                                                                                                     >  2:94533/1‑139 (MQ=255)
  tAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTcc                                                                                                                                     >  2:497272/1‑139 (MQ=255)
    aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgc                                                                                                                                   <  2:535062/139‑1 (MQ=255)
      cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgccg                                                                                                                                 >  1:35215/1‑139 (MQ=255)
           cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac                                                                                                                             >  1:239843/1‑138 (MQ=255)
           cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac                                                                                                                             >  2:451805/1‑138 (MQ=255)
           cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac                                                                                                                             >  1:732452/1‑138 (MQ=255)
               tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc                                                                                                                        >  1:226833/1‑139 (MQ=255)
                cgggCAATCCCGGCCGCAGCCCTGTGTCAGCGCCTCCCGTGATTCATTTCCCCCAACACCCGCGCATTTACCCACCCCCTTGTCCAAAAA‑TGCCTCCGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAACAAAAcc                                                                                                                       <  2:338724/137‑1 (MQ=255)
                cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc                                                                                                                       >  1:586260/1‑139 (MQ=255)
                cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc                                                                                                                       >  1:606963/1‑139 (MQ=255)
                      ttcccggCCGCAGCCCTGTGTCAGCGGCTCCCGTGTTTCATTCCCGCAAACACCCGCCCTTTTACCCAACCCCATGTGCAAAAA‑TGCCTCCGCGGCGGCTGTCGGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGtt                                                                                                                 <  1:660913/133‑1 (MQ=18)
                        tCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtct                                                                                                               >  1:364501/1‑139 (MQ=255)
                        tCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtct                                                                                                               >  1:293012/1‑139 (MQ=255)
                            gCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc                                                                                                            >  1:74918/1‑138 (MQ=255)
                            gCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc                                                                                                            >  1:812740/1‑138 (MQ=255)
                                  gACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagtagt                                                                                                     >  2:90603/1‑139 (MQ=255)
                                    ccggtgcCAGCGTCTCCCGTGATTCTTTCCCCCCAACACCCGCGCTTTTACCCAACCCCATGTGAAAAAA‑TGCCTCCGGGGCGGCTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                   <  1:917033/136‑1 (MQ=255)
                                          tCAGCGCCTCCCGTGATTAATTCCCGCCAACACCCGCGCATTTACCCAACGCCATGTAAAAAAA‑TGCCTTCGGGGCGGCTGTCTGCCAGCTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc                                                                                             <  1:497272/139‑1 (MQ=21)
                                           cggcgcCTCCCTTGATTCTTTCCCCCAAACACCCGCTCTTTTACCCACCGCCTTGTGAAAAAA‑GCCCTTCGGGGCGGCTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa                                                                                            <  1:554527/137‑1 (MQ=255)
                                           cAGCGGCTCCCGTAATTCATTCCCCCCAACACCCGCGCATTTACCAACCGCCTGGCAAAAAAA‑TGCCTCCGGGGCGGCTGTCGCCAAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa                                                                                            <  2:606963/139‑1 (MQ=255)
                                             gCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGcg                                                                                            >  1:774909/1‑136 (MQ=255)
                                             gCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAag                                                                                          >  1:565404/1‑139 (MQ=255)
                                               ggCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgc                                                                                                                                   >  2:107004/1‑96 (MQ=255)
                                               ggCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgc                                                                                                                                   <  1:107004/96‑1 (MQ=255)
                                                  tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAaggg                                                                                        >  1:139546/1‑134 (MQ=255)
                                                  tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAG‑aa                                                                                     >  1:94814/1‑138 (MQ=255)
                                                  ccccGTGATTCTTTCCCCCCAACACCCGCGCTTTTATCAACCCCCATGGAAAAAAA‑CGCCTCCGGGGCGCCTGTCTGCAGGTTTTTTTCCCGCCAAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑aaa                                                                                    <  2:732452/137‑1 (MQ=255)
                                                    ccGTGTTCCATTCCCCCCAACACCCGCGCTTTTACCAAACCCAATGTGAAAAAA‑TGCCTCCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAAt                                                                                   <  2:475195/138‑1 (MQ=255)
                                                             aTTCCCGCCAAAACCCGCCATTTTATCCAACGCCATGTAAAAAAA‑TGCCTCCGCGGGGGCTGTCTGCCAGTTATTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGc                                                                         <  1:382589/139‑1 (MQ=255)
                                                               tccccccAACACCCGCCCATTTACCCACCCCCATGTGAAAAAT‑TCCCTCCGCGGGGGCTTTCTGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGc                                                                         <  1:451805/132‑1 (MQ=255)
                                                                  caccAAAACCCGCGCTTTTATCCAACGCCAGGTGAAAAAA‑TGCCGGCGGGGCGCCTGTCGGCCTGTTATTTTCCCCCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTc                                                                    <  2:35215/137‑1 (MQ=255)
                                                                  cGCCAACACCCGCGCATTTATCCACCCCCATGTGCAAAAA‑TGCCTCCGCGGCGGCTGTCTCCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGAGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTc                                                                    <  1:824931/139‑1 (MQ=255)
                                                                  cGCCAACAACCGCGCTTTTACCCAACGCCATGCAAAAAAA‑TGCCTCCGGGCCGGCGGTCTGCAGTCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTc                                                                    <  2:289586/139‑1 (MQ=255)
                                                                     caacacCCGCGCTTTTACCCAACGCAATG‑GCAAAAATTGCTGTCGGGGCGCTTGTCTGCCAGTTATTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtg                                                                 <  2:586260/139‑1 (MQ=255)
                                                                     caacacCCGCGCATTTACCCACCTCCATGTAAAAAAA‑TGCTATCGCGGCGTCTGTCTCCCTCCTTTTTTACCGCCGCAACAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtg                                                                 <  1:604302/139‑1 (MQ=255)
                                                                                 cattACCCACCGCCGTGTTAAAAAA‑TCCCTTCGCGCCGGCTGTCTGCCACCTATTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAAt                                                     <  1:96106/137‑1 (MQ=255)
                                                                                    ttcccACCGCCATGTGCAAAAT‑TCCCTCCGCGGCGGCTGTCCGCCAGCTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCg                                                   <  1:94533/135‑1 (MQ=255)
                                                                                    tacccAACGCCATGTAAAAAAA‑CCCCTGCGCGGCGTCTGTCGGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCg                                                   <  2:226833/135‑1 (MQ=255)
                                                                                     aTCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAGAG‑GAAAAACTTTTAACCCCCCCTTTTTTGTGGGGATCgct                                                 >  1:720512/1‑139 (MQ=25)
                                                                                      cccAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGATTTAAGCCCGTCATGTTGTGTGGCAATCgctg                                                <  1:833828/138‑1 (MQ=255)
                                                                                      cccAACCCCATGCACAAAAT‑CCCCTTCGCGGGCGCGATCGGCCAGCTTTTTTCCCGCCACAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctg                                                <  2:293012/138‑1 (MQ=255)
                                                                                             ccATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAAAAAATTTTAGCCCGCCATGTTTTGTGGGGATCgccgctcggt                                          >  1:541730/1‑134 (MQ=37)
                                                                                                    aaaaaa‑TGCCTTCGCGGCGGCTGTCTGCCATCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtgt                                   <  1:521710/137‑1 (MQ=255)
                                                                                                             ccTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAAAATTTAAACCC‑ACATGTTGTGGTGGAGACGCTGCTGGGAAAAAAGAGGGAAgggg                          >  2:514868/1‑138 (MQ=255)
                                                                                                             ccTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGACGGG‑AAAAAAATTTTAGACCCCCATGTTGTGGGGGGATCGGTGCTGGGGAAAAAGTGGGAAggg                           >  2:71135/1‑138 (MQ=255)
                                                                                                                 cgcgGCGGCTTTCGGCCGGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAg                        <  2:627512/137‑1 (MQ=255)
                                                                                                                   cggcggCTGTCGGCCAGCTATTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcg                    <  1:720429/139‑1 (MQ=255)
                                                                                                                            tttGCCTGCTATTTTCCCCCCCCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc             <  1:622715/135‑1 (MQ=255)
                                                                                                                            tcggCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc             <  2:191368/134‑1 (MQ=255)
                                                                                                                            tCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc             <  1:79097/137‑1 (MQ=255)
                                                                                                                            cccgCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc             <  2:929053/134‑1 (MQ=255)
                                                                                                                                    ttATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                   <  1:337536/43‑1 (MQ=255)
                                                                                                                                    cTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                   >  2:337536/1‑44 (MQ=255)
                                                                                                                                      tttttcCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc  <  1:661759/137‑1 (MQ=255)
                                                                                                                                      aTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc  <  1:125453/138‑1 (MQ=255)
                                                                                                                                      aTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc  <  2:27789/138‑1 (MQ=255)
                                                                                                                                      aTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc  <  2:619942/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAC  >  NZ_CP009273/353453‑353723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑