| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,429,984 | 0 | G | A | 39.1% | 27.2 / 11.8 | 23 | intergenic (‑108/+33) | uspF/ompN | universal stress protein UspF/porin OmpN |
| Reads supporting (aligned to +/- strand): ref base G (13/1); new base A (0/9); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCC‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGT > NZ_CP009273/1429849‑1430117 | cGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCC‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa > 2:470828/1‑138 (MQ=255) aGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCC‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGc > 1:683792/1‑139 (MQ=255) aaaaacCCTCTTTTTTTAGTCCCCGCAAAAAGT‑TAACCCCAAGATAACATTTTTTGTGTAAGGAGGGGGTTCACTTTTTAAATTGAGATAAAGGGAGAAAAAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCagga < 2:703910/136‑1 (MQ=255) aTAAACCCTCTTGTTTTAGTATCCGAATAAAGTTTAACGCC‑AGATGACACTTTTTTTGTAATGACGGATTTCACTTTTTTAATTTAGATAAAGGGAGAAAGAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCagga < 1:708594/138‑1 (MQ=255) ctGTTTTAGTATCCGCATAAAGTGTAACGCC‑AGATGACATTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAAAAAGCAGAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGa < 1:437536/137‑1 (MQ=255) ctGTTTTAGTATCCGCATAAAGTGTAACGCC‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAAc > 2:704441/1‑139 (MQ=255) ctGTTTTAGAATCCGAAAAAAGTGAAACGCC‑AGATGACACTTTTTGTGAAATGACGGAGTTCACATTTTTAATTAAGATAAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGa < 1:867725/137‑1 (MQ=255) tCCGCATAAAGTGTAACGCC‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAc > 2:5838/1‑136 (MQ=255) cGCATAAAGTGTAACGCC‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAc > 1:439107/1‑139 (MQ=255) cGCATAAAGTGTAACGCC‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAc > 2:659391/1‑139 (MQ=255) cGCATAAAGTGTAACGCC‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGAAGGAGGATTTAGAACTGATAAACCAGAc > 2:695043/1‑139 (MQ=255) gCC‑AGATAACATTTTTTGTGTAAGGGCGGAGTTCACTTTTTAAATTTAAAAAAAAGGGGGAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAatat < 2:688451/139‑1 (MQ=255) c‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatca > 1:842587/1‑139 (MQ=255) aGATGACTTTTTTTGTGTAATGACGGAGTTCACTTTTTTAATTAAGAAAAAGGAAGAAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatcat < 2:668321/139‑1 (MQ=255) tGACATTTTTTGTGTAATGACGGAGTCCACTTTTTAAATTAAGAAAAAAGGAGAAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCgg < 2:613453/139‑1 (MQ=255) tAATGACGGAGTTCACATTTTTAGTTTAGATCAAAGGAGGAAGACTAAGCAGAAAAAGCCCGCCCTAACATCGGGCGGGAGGATTTAGAACTGATAAAACAGACCTAAAGCGACAATATCCTCGGTAGAGATGCCATTg > 2:525197/1‑139 (MQ=255) aTGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTgg > 1:724331/1‑138 (MQ=255) ttCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAg > 1:360219/1‑137 (MQ=255) taaaTTAAGAAAAAAGGAGAAAAAAAAAGCAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCAt < 2:424751/136‑1 (MQ=255) aaGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAgg > 2:731538/1‑139 (MQ=255) aggaggAAGAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGt < 1:5838/139‑1 (MQ=255) aggaggAAAAATAAAAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGt < 1:217242/139‑1 (MQ=255) aGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGt > 1:522132/1‑138 (MQ=255) | CGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCC‑AGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGT > NZ_CP009273/1429849‑1430117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |