| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,571,650 | 0 | A | C | 45.7% | 47.2 / 11.1 | 35 | V146G (GTG→GGG) | gntR | gluconate operon transcriptional repressor GntR |
| Reads supporting (aligned to +/- strand): ref base A (15/4); new base C (0/16); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAC > NZ_CP009273/3571514‑3571782 | gtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTccac < 1:185073/138‑1 (MQ=255) tcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTccacca > 2:104646/1‑139 (MQ=255) tcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTccacca > 1:483292/1‑139 (MQ=255) aCGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGa < 2:323605/139‑1 (MQ=255) gcgcCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑c > 2:514173/1‑139 (MQ=255) cAATGTGGCGATGCCCGCGAGAAATAATGGCAGTGGTCATCGGGCGGGCTGCTCAAAATTTATCAAAACCGCCGGCGATAT‑AAGGGATGGGCGATTTGCTGTCCATCATTCCCCCCAGGGGAATA‑CCCGCCACTTCAat < 1:665045/139‑1 (MQ=255) cAATGTGGCGATCCCCGCGAGAAATAATGGCAGTGGTCATCTGGGGTGCTGCTTAAAAGTTATCAAACCCGCGGGCGATATCAAGGCAG‑GGCGACTTGCTGTCCATCAGTCCCCCCACGGAAATC‑CCCGCCACTTCAat < 1:159587/139‑1 (MQ=255) cAATGGGGCGATGCCCGCGAGAAATAAGGGCAGTGGTCATTGGGCGTGCTGCTTAAAAGTTAAAAAAACCGACGGCGATATAAAGGCAG‑GGCGACTTGCTGCCCATCAGTCCCCCCAGGGGAATA‑CCCGCCACTTCAat < 1:100206/139‑1 (MQ=255) cAATGGGGCGAGGCCCGCGAGAAATAATGGCAGTGGTCATCTGGCGTGCTGTTAAAAAGTTATAAAACCCGCCGGCGATATCAAGGCAT‑GGCGACTTGCTGCCCATCAGTTCCCCCACGGAAATA‑CCCGCCACTTCAat < 1:514173/139‑1 (MQ=255) cAAGGTGGCGAGGCCCGGGAGAAATAAGGGCAGGGGTCCTCTGGCGTGCTGCTTAAAAGTTAACAAAACCGACGGCAAAACCAAGGCAT‑GGCGACTTCCTCTCCACAAGTCCCCCCACGGGAATA‑CCCGCCACTTCAat < 2:43518/139‑1 (MQ=255) aattggggCGTTCCCCGCGAGAAATAGTGGCGGTGGTCATTGGGCGTGCTGTTTCAAATTTAAAAAAACCGACGGCGATATCAAGGCTT‑GGCGTTTTCCTGTCCATCGGTCCCCCCACGGAAATC‑CCCGCCACTTCAat < 2:635537/133‑1 (MQ=255) aagtgtgGCGATGCCCGCGAGAAATAAGGGCATGGTTCACCGGGCGTGCTTCTGAAAATTTACAAAACCCGACGGCGATATCAAGGCAG‑GGCGTCTTGCTGCCCATAAGTCCCACCAGGGGAATC‑CCCGCCACTTCAat < 2:256443/136‑1 (MQ=255) cGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCttt > 2:740819/1‑138 (MQ=255) cgAGCAATAGGGGAAGTGGTCTTCTGGCGTGCTGCTTAAAATTTAACAAACCCGCCGGCGATACCAGGGCAT‑GCCGACTTGCTGTCCATCATTCCCACCCCGGAAATC‑CCCGCCACTTCAATCATCTTTAAGGTGcgcg < 2:36877/139‑1 (MQ=255) cgAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGcgcg > 2:860272/1‑139 (MQ=255) cgAGAAATAAGGGGGGTGGTCATCGGGCGTGCTGCTTAAAAGTTATCAAACCCGACGGCGATAAAAAGGCGT‑GGCGCCTTGCTGCCCATCAGTCCCCCCACGGAAATA‑CCCGCCACTTCAATCATCTTTAAGGTGcgcg < 1:104646/139‑1 (MQ=255) cgAGAAATAAGGGCGGTGGTCTTCTGGCGTGTTGTTAAAAATTTAAAAAACCCGACGGCAATATCAGGGCAT‑GGCGATTTGCTGCCCATCAGTCCCCCCACGGAAATC‑CCCGCCACTTCAATCATCTTTAAGGTGcgcg < 1:215281/139‑1 (MQ=255) cgAACAATAAGGGGGGGGGTCATCTGGCGTGCTGTTAAAATGTTATAAAAACCGCGGGTGATAAAAAGGCAT‑GGCGTTTTGTTGTCCAGCGTTCCCACCAGGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGcgcg < 2:937916/139‑1 (MQ=255) ataatGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgtgt > 2:229738/1‑139 (MQ=255) cAGTGGTCATCGGGCGTGCTGCTTAAAAGTTATAAAACCCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCATTCCCCCCGGGGGAATC‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACg < 1:511984/139‑1 (MQ=255) gTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGtt > 2:430194/1‑139 (MQ=255) tCATTTGGCGTGCTGCTTAAAATTTACAAAACCCGACGGCGATATAAAGGCAT‑GGCGTTTTGCTGTCCATCAGTCCCCCCACGGGAATC‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGt < 1:155967/139‑1 (MQ=255) tgctgcTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAgg > 2:727107/1‑139 (MQ=255) ctTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTc > 1:708631/1‑139 (MQ=255) ctTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTc > 1:422557/1‑139 (MQ=255) aaaaGTTAAAAAACCCGACGGCGATACAAAGGCAT‑GGCGATTTGCTGCCCACCAGTCCCCCCACGGAAATC‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGa < 1:860272/137‑1 (MQ=255) aaaaaacCCGACGGGAATACCAGGGCAG‑GGCGCCTTGCTGCCCATCAGTCCCCCCAGGGGAATC‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg < 2:926373/136‑1 (MQ=255) aTCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg > 2:218358/1‑139 (MQ=255) taaaacCCGGCGGCGATAAAAAGGCAT‑GGCGACTTGCTGCCCATCAGTCCCCCCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg < 1:725871/136‑1 (MQ=255) cGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt > 2:253057/1‑139 (MQ=255) cGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGa > 1:926373/1‑139 (MQ=255) cGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGa > 2:694968/1‑139 (MQ=255) ggCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCt > 1:324590/1‑139 (MQ=255) gCTGTCCATCAGTTCCCCCACGGGAATC‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTc < 1:210257/138‑1 (MQ=255) gCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTcc > 1:429552/1‑139 (MQ=255) ggTCCAGCCGTCCCCCCGCGGGAACC‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTc < 1:576364/135‑1 (MQ=255) cAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATaa > 1:718470/1‑139 (MQ=255) agtCCCCCCCGGGAAAACCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAc < 2:774955/137‑1 (MQ=255) | GTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCAT‑GGCGACTTGCTGTCCATCAGTTCCACCACGGGAATA‑CCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAC > NZ_CP009273/3571514‑3571782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |