Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→G	W30G (TGG→GGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	G	100.0%	166.6 / NA	46	W30G (TGG→GGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/19);  total (27/19)

TTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACG  >  NZ_CP009273/49774‑50041                                                                                                                                         |                                                                                                                                    tttACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCggg                                                                                                                                   >  2:275254/1‑139 (MQ=255)          tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACg                                                                                                                           >  1:254453/1‑138 (MQ=255)          tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACg                                                                                                                           >  2:915611/1‑138 (MQ=255)          tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACg                                                                                                                           >  1:390503/1‑138 (MQ=255)          tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGc                                                                                                                          >  2:769608/1‑139 (MQ=255)          tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGGGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACg                                                                                                                           >  1:417788/1‑138 (MQ=255)            cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                                                                        <  2:24773/139‑1 (MQ=255)            cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCa                                                                                                                         <  2:663735/138‑1 (MQ=255)                taGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                                                                        >  2:674405/1‑135 (MQ=255)                taGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                                                                        <  1:674405/135‑1 (MQ=255)                taGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACAcc                                                                                                                    <  1:275254/139‑1 (MQ=255)                    ggCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATAGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTta                                                                                                                 >  2:837122/1‑138 (MQ=255)                      cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaa                                                                                                               >  1:479350/1‑138 (MQ=255)                      cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaa                                                                                                               >  2:358770/1‑138 (MQ=255)                      cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaa                                                                                                               >  1:593346/1‑138 (MQ=255)                                     tCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg                                                                                               <  2:390503/139‑1 (MQ=255)                                     tCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg                                                                                               <  1:763645/139‑1 (MQ=255)                                       gggAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                                                             <  1:873457/139‑1 (MQ=255)                                          aaaTCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                                                                                          <  1:915611/139‑1 (MQ=255)                                                  aTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                                                                  <  2:479350/139‑1 (MQ=255)                                                        aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaat                                                                            >  1:926644/1‑139 (MQ=255)                                                        aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaat                                                                            >  2:144961/1‑139 (MQ=255)                                                        aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaat                                                                            >  2:360391/1‑139 (MQ=255)                                                         gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAGCGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaat                                                                            >  1:467544/1‑138 (MQ=255)                                                         gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaat                                                                            >  1:495281/1‑138 (MQ=255)                                                                tGCGGCGTTAGCGGTAGATCGCTTTATGGGCATGGAAAACGCCGTCCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg                                                                     <  2:520371/138‑1 (MQ=255)                                                                 gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt                                                                    <  1:469452/138‑1 (MQ=255)                                                                    gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                                                                                      >  2:742145/1‑101 (MQ=255)                                                                    gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                                                                                      <  1:742145/101‑1 (MQ=255)                                                                    gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc                                                                >  1:384850/1‑139 (MQ=255)                                                                    gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc                                                                >  1:820516/1‑139 (MQ=255)                                                                               aGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAAACGGTCGTCCGTTGCCAGGa                                                     >  1:799724/1‑139 (MQ=255)                                                                                 aTCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                                                              <  1:453474/96‑1 (MQ=255)                                                                                 aTCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                                                              >  2:453474/1‑96 (MQ=255)                                                                                     cgTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGGTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                                >  2:796263/1‑138 (MQ=255)                                                                                                          cATGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCaa                          <  1:358770/139‑1 (MQ=255)                                                                                                            tGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAc                                                                                                          <  2:432205/57‑1 (MQ=255)                                                                                                            tGCAGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAc                                                                                                          >  1:432205/1‑57 (MQ=255)                                                                                                                   gAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACg                 >  2:635856/1‑139 (MQ=255)                                                                                                                      cctgcctgCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGAc              >  1:64433/1‑139 (MQ=255)                                                                                                                       ctgcctgcCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACg             <  2:820516/139‑1 (MQ=255)                                                                                                                       ctgcctgcCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACg             <  1:144961/139‑1 (MQ=255)                                                                                                                         gcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGAt           >  1:64875/1‑139 (MQ=255)                                                                                                                           ctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg                                                                               <  1:96142/69‑1 (MQ=255)                                                                                                                           ctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg                                                                               >  2:96142/1‑69 (MQ=255)                                                                                                                              ccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATcgcg       >  1:542874/1‑138 (MQ=255)                                                                                                                                      gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACg  <  2:384850/135‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACG  >  NZ_CP009273/49774‑50041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑