Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,576,900	(A)6→5	intergenic (‑119/+283)	ydeN ← / ← ydeO	sulfatase/acid stress response transcriptional regulator YdeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,576,895	0	A	.	100.0%	144.4 / NA	33	intergenic (‑114/+288)	ydeN/ydeO	sulfatase/acid stress response transcriptional regulator YdeO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (14/19);  total (14/19)

TTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCATTTTACTGACAAAATGCAGAATTGAGATCATAAA  >  NZ_CP009273/1576760‑1577026                                                                                                                                        |                                                                                                                                    ttttCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGaaa                                                                                                                                   >  2:828674/1‑138 (MQ=255)                      tAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACa                                                                                                            <  1:1167957/139‑1 (MQ=255)                            ttCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACtt                                                                                                      >  2:275544/1‑139 (MQ=255)                              ccTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACttt                                                                                                     <  1:294081/138‑1 (MQ=255)                               ctctTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCt                                                                                                   >  2:531443/1‑139 (MQ=255)                                  ttcttATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTg                                                                                                  <  1:789040/137‑1 (MQ=255)                                    cttATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGtt                                                                                                <  1:506660/137‑1 (MQ=255)                                       aTTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTa                                                                                             <  2:1212210/137‑1 (MQ=255)                                         tGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTAtt                                                                                           >  2:834436/1‑137 (MQ=255)                                             cTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGaaaaaca                                                                                                                               <  2:795026/98‑3 (MQ=255)                                             cTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGaaaaaca                                                                                                                               >  1:795026/1‑96 (MQ=255)                                             cTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACAtt                                                                                                          <  2:437989/118‑1 (MQ=255)                                             cTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACAtt                                                                                                          >  1:437989/1‑118 (MQ=255)                                             cTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTaa                                                                                     >  1:944769/1‑139 (MQ=255)                                                  ttCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTtct                                                                                <  2:1169083/139‑1 (MQ=255)                                                     cAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGc                                                                             >  2:526130/1‑139 (MQ=255)                                                          tAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATa                                                                        <  1:1150164/139‑1 (MQ=255)                                                             ttttACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAaac                                                                     <  1:584752/139‑1 (MQ=255)                                                                tACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAaacaa                                                                   <  1:794813/138‑1 (MQ=255)                                                                    aaGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAAt                                                              >  1:873589/1‑139 (MQ=255)                                                                              aGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGata                                                     <  1:834436/138‑1 (MQ=255)                                                                                   aTGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGat                                                      >  1:433408/1‑132 (MQ=255)                                                                                   aTGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGat                                                      <  2:433408/132‑1 (MQ=255)                                                                                         gTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAatta                                         <  2:130090/139‑1 (MQ=255)                                                                                                 aGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCAttt                                 >  1:97596/1‑139 (MQ=255)                                                                                                  gTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCAttt                                 >  2:681094/1‑138 (MQ=255)                                                                                                          aaGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCATTTTACTGACa                         <  2:676183/138‑1 (MQ=255)                                                                                                                 aGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATa                                                                        >  1:803640/1‑84 (MQ=255)                                                                                                                 aGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATa                                                                        <  2:803640/84‑1 (MQ=255)                                                                                                                 aGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCATTTTACTGACAAAATg                    >  1:212576/1‑136 (MQ=255)                                                                                                                 aGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCATTTTACTGACAAAATg                    >  1:76010/1‑136 (MQ=255)                                                                                                                     gATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCATTTTACTGACAAAATGCAGAAtt             <  2:1159685/139‑1 (MQ=255)                                                                                                                     gATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCATTTTACTGACAAAATGCAGAAtt             <  2:705268/139‑1 (MQ=255)                                                                                                                         tAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACttt                                                                                                     <  2:172402/47‑1 (MQ=255)                                                                                                                         tAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACttt                                                                                                     >  1:172402/1‑47 (MQ=255)                                                                                                                                aaaaaGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCATTTTACTGACAAAATGCAGAATTGAGATCataaa  <  2:353686/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCATTTTACTGACAAAATGCAGAATTGAGATCATAAA  >  NZ_CP009273/1576760‑1577026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑