Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,376,303	(A)8→7	coding (112/1212 nt)	elaD →	deubiquitinase ElaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,376,296	0	A	.	100.0%	173.2 / NA	39	coding (105/1212 nt)	elaD	deubiquitinase ElaD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/29);  total (10/29)

CACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTTAAAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCTTTACCATATTGTTAGAACCCC  >  NZ_CP009273/2376165‑2376415                                                                                                                                    |                                                                                                                        cacaACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTTaaaaaaa                                                                                                                   >  2:990586/1‑138 (MQ=255)           gCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATcc                                                                                                       >  1:19414/1‑139 (MQ=255)               cTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGgag                                                                                                   <  2:481111/139‑1 (MQ=255)                 aaaaGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGAt                                                                                                 >  2:912783/1‑139 (MQ=255)                  aaaGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGAt                                                                                                 <  1:189435/138‑1 (MQ=255)                  aaaGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGAt                                                                                                 >  1:314325/1‑138 (MQ=255)                  aaaGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGAt                                                                                                 >  1:955873/1‑138 (MQ=255)                           cATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCa                                                                                       <  1:560226/139‑1 (MQ=255)                                 ggTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGt                                                                                 >  1:673444/1‑139 (MQ=255)                                  gTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGt                                                                                 <  1:187406/138‑1 (MQ=255)                                  gTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGt                                                                                 <  2:628140/138‑1 (MQ=255)                                  gTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGt                                                                                 <  1:178142/138‑1 (MQ=255)                                  gTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGt                                                                                 <  2:292897/138‑1 (MQ=255)                                  gTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGt                                                                                 <  1:640967/138‑1 (MQ=255)                                   ttACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTTa                                                                                                                         >  1:20873/1‑98 (MQ=255)                                   ttACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTTa                                                                                                                         <  2:20873/98‑1 (MQ=255)                                   ttACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTa                                                                               <  1:206018/139‑1 (MQ=255)                                   ttACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTa                                                                               <  1:227146/139‑1 (MQ=255)                                        gTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACTTTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCa                                                                          <  1:1278512/139‑1 (MQ=255)                                        gTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCa                                                                          <  2:545847/139‑1 (MQ=255)                                            tCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCataa                                                                       >  1:822955/1‑138 (MQ=255)                                              aGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTTaaaaaaa                                                                                                                   <  2:775913/93‑1 (MQ=255)                                              aGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTTaaaaaaa                                                                                                                   >  1:775913/1‑93 (MQ=255)                                              aGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCataata                                                                     <  1:259123/138‑1 (MQ=255)                                              aGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCataata                                                                     <  2:1154829/138‑1 (MQ=255)                                              aGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCataata                                                                     <  2:673444/138‑1 (MQ=255)                                              aGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAatat                                                                    >  2:337824/1‑139 (MQ=255)                                              aGCAAGTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGGGACCGAGGAATTGCTGCAGTCTGT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCAGTAGGTTACTGCataaga                                                                     <  2:260842/138‑3 (MQ=255)                                                        gTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAgg                                                           <  1:912783/138‑1 (MQ=255)                                                                tctcAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATa                                                   <  1:337824/138‑1 (MQ=255)                                                                                 tcagACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCg                                                                                                      <  1:586772/70‑1 (MQ=255)                                                                                 tcagACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCg                                                                                                      >  2:586772/1‑70 (MQ=255)                                                                                            cAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCt                       <  2:955873/138‑1 (MQ=255)                                                                                            cAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCt                       <  2:314325/138‑1 (MQ=255)                                                                                                aaaaTTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCTTTAcc                  <  1:392949/139‑1 (MQ=255)                                                                                                aaaaTTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCTTTAcc                  <  1:580664/139‑1 (MQ=255)                                                                                                aaaaTTTAATGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCTTTccc                  <  2:268225/139‑1 (MQ=255)                                                                                                   aTTTACTGTGACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCTTTACCata               <  2:218532/139‑1 (MQ=255)                                                                                                           tgACCGAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCTTTACCATATTGTTAg        <  1:84918/138‑1 (MQ=255)                                                                                                                gAGGAATTACTGCAGTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCTTTACCATATTGTTAGAAcccc  <  1:863979/139‑1 (MQ=255)                                                                                                                              gTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCa                                                                          >  1:362228/1‑53 (MQ=255)                                                                                                                              gTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCa                                                                          <  2:362228/53‑1 (MQ=255)                                                                                                                              gTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGt                                                               >  2:1237758/1‑64 (MQ=255)                                                                                                                              gTCTTT‑AAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGt                                                               <  1:1237758/64‑1 (MQ=255)                                                                                                                                    |                                                                                                                        CACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACGCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGACCGAGGAATTACTGCAGTCTTTAAAAAAAACAGCGTTATCCGGAGATGAAGAAAGCATTGAGTTACTGCATAATATTGCGTTAGGTTATGATAAATTTGGGAAAGAAGCTGAAGATATTCTTTACCATATTGTTAGAACCCC  >  NZ_CP009273/2376165‑2376415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑