| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,497,341 | (C)5→4 | coding (624/2496 nt) | ptsP ← | phosphoenolpyruvate‑‑protein phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,497,337 | 0 | C | . | 92.5% | 154.7 / 7.2 | 41 | coding (628/2496 nt) | ptsP | phosphoenolpyruvate‑‑protein phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base . (21/16); total (23/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CGGCTTCAGTTCCGGCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAATTGCTGGTT > NZ_CP009273/2497200‑2497466 | cgGCTTCAGTTCCGGCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCgccgcc > 2:96516/1‑139 (MQ=255)cgGCTTCAGTTCCGGCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCgccgcc < 1:1270377/139‑1 (MQ=255)cgGCTTCAGTTCCGGCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCgccgcc > 2:781553/1‑139 (MQ=255) ggCTTCAGTTCCGGCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGccc > 1:1264264/1‑139 (MQ=255) ggCTTCAGTTCCGGCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGccc > 2:425568/1‑139 (MQ=255) cTTCAGTCCCGGCCATGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGATAACTCGTTTGCGCAGATAATCGCTGGCTGACGCAGAAAGTTTGGCGCAAACCTGCTCCATATTCCTGATGAGCGCCGcccca < 2:663729/139‑2 (MQ=255) cAGTTCCGGCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGTTGATCGCCGCCCCCAGc > 2:271866/1‑139 (MQ=255) ggCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATg < 1:27193/139‑1 (MQ=255) ggCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATg < 2:531885/139‑1 (MQ=255) ggCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATg > 1:531885/1‑139 (MQ=255) ggCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATg > 2:969110/1‑139 (MQ=255) ggTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGtt < 2:415464/139‑1 (MQ=255) ggTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGtt < 1:951253/139‑1 (MQ=255) gTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTc > 2:194103/1‑139 (MQ=255) gcaACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGccc < 2:1280526/111‑1 (MQ=255) gcaACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGccc > 1:1280526/1‑111 (MQ=255) gcaACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATg > 2:347771/1‑123 (MQ=255) gcaACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATg < 1:347771/123‑1 (MQ=255) aCTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGa < 1:194103/139‑1 (MQ=255) aCTGGTCGCTGATATCGCGAATGTCGATAGCACGTTCGCGCAGAGAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGGTTATGATCG‑CGGCCACAGCCCCTGATGCTGTTCTGTCATCA‑GGGGa < 2:674418/139‑1 (MQ=255) ttCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACgg < 1:999075/138‑1 (MQ=255) ttCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGa > 1:761440/1‑139 (MQ=255) cgAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCaaa > 1:443941/1‑139 (MQ=255) tCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATtcat < 1:425568/138‑1 (MQ=255) ttCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑g > 1:901182/1‑101 (MQ=255) ttCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑g < 2:901182/101‑1 (MQ=255) tCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGa < 1:766996/104‑1 (MQ=255) tCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGa > 2:766996/1‑104 (MQ=255) cgcgCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGc > 2:449764/1‑139 (MQ=255) gcagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCgccgcc < 1:705567/54‑1 (MQ=255) gcagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCgccgcc > 2:705567/1‑54 (MQ=255) gcagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGtt < 1:1203411/72‑1 (MQ=255) gcagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGtt > 2:1203411/1‑72 (MQ=255) cagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAgg < 2:761440/139‑1 (MQ=255) gcAAACCTGCTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGt < 1:393564/139‑1 (MQ=255) cTCCATATTGCTGATGATCGCCGCCCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGtcacgctca > 2:612957/1‑139 (MQ=255) cTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATtcat > 1:970570/1‑79 (MQ=255) cTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATtcat < 2:970570/79‑1 (MQ=255) cTCCATATTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGtcacgctcac > 2:930779/1‑139 (MQ=255) tatTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAAt > 1:198880/1‑139 (MQ=255) tatTGCTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGAAAGTTTTGATTTCGCCGTCACGCTCACGCAAt > 1:1160578/1‑139 (MQ=255) cTGATGATCGCCGCCCCCAGCCCCTGATGCTGTTCTGTCATCAGGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAATTg < 1:1251901/138‑1 (MQ=255) cTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAATTGCTg > 1:854354/1‑139 (MQ=255) cTGATGATCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAATTGCTg > 1:616820/1‑139 (MQ=255) atgatCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGtc > 1:80797/1‑118 (MQ=255) atgatCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGtc < 2:80797/118‑1 (MQ=255) atgatCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAATTGCTGGtt > 1:530921/1‑139 (MQ=255) gatCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAATTGc > 2:907057/1‑132 (MQ=255) gatCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAATTGc < 1:907057/132‑1 (MQ=255) gatCGCCG‑CCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAATTGCTGGtt > 2:831771/1‑137 (MQ=255) gatCGCCG‑CCCCAGCCCCTGATGCTGTTCTGCCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCGCACGCAATTGCTGGtt > 2:546518/1‑137 (MQ=255) cgccg‑cCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATgg > 1:873457/1‑84 (MQ=255) cgccg‑cCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATgg < 2:873457/84‑1 (MQ=255) | CGGCTTCAGTTCCGGCCAGGTGATATGCAGCAACTGTTCGCTGATATCGCGAATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCTGATGCTGTTCTGTCATCA‑GGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCGCCGTCACGCTCACGCAATTGCTGGTT > NZ_CP009273/2497200‑2497466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |