| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 306,481 | (T)5→4 | coding (519/543 nt) | ecpR ← | ECP biosynthesis operon DNA‑binding transcriptional regulator EcpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 306,477 | 0 | T | . | 91.1% | 158.3 / 12.2 | 45 | coding (523/543 nt) | ecpR | ECP biosynthesis operon DNA‑binding transcriptional regulator EcpR |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base . (14/27); total (15/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GTAAACACCGTTACCAGAGCTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCG > NZ_CP009273/306339‑306611 | gTAAACACCGTTACCAGAGCTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTatatat > 2:80244/1‑139 (MQ=255)gTAAACACCGTTACCAGAGCTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTatatat > 1:1259552/1‑139 (MQ=255) tAAACACCGTTACCAGAGCTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATAtt < 2:918169/139‑1 (MQ=255) ccGTTACCAGAGCTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATAttttgagt < 1:957219/139‑5 (MQ=255) cGTTACCAGAGCTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTa < 1:309465/139‑1 (MQ=255) ccAGAGCTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGc < 1:423430/138‑1 (MQ=255) ccAGAGCTATTGCCAGAACC‑TTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGc < 1:371655/138‑1 (MQ=255) cAGAGCTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACCAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCtt < 2:5021/139‑1 (MQ=255) cTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCt < 1:1091916/139‑1 (MQ=255) ttGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAg < 1:1139977/139‑1 (MQ=255) ttGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAg > 2:911240/1‑139 (MQ=255) aGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTa > 1:254930/1‑139 (MQ=255) gAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTAc < 2:256116/139‑1 (MQ=255) aaCCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACg > 2:167592/1‑139 (MQ=255) cTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGcc > 1:1261549/1‑138 (MQ=255) gttcttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 2:62334/135‑1 (MQ=255) tttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGAt > 2:1211044/1‑139 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 2:913309/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 2:1261549/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 2:1032759/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 2:319576/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 2:685878/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 1:1086985/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 2:848939/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 2:887348/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 1:381926/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 1:597502/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 1:92675/137‑1 (MQ=255) ttttttCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGa < 1:629713/137‑1 (MQ=255) ttttttCATTGCATTTCATCCCGAGTTGAATTGACGACGTGATGTCGCAGTGTGTCATTTTTTAAAACTAACGTGTCTGGCGTTTACTGACTCCACTTATATA‑CTTTGAGTACAGCTTGGCCTCCGCATTCCTCCGa < 2:725800/137‑1 (MQ=255) gCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATacac < 1:1066459/138‑1 (MQ=255) gCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATacac < 2:1259552/138‑1 (MQ=255) cttcCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCtt < 1:1204500/138‑1 (MQ=255) gTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTgg > 2:602159/1‑139 (MQ=255) aTGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCaa < 2:1284389/139‑1 (MQ=255) ccAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCtt > 2:557079/1‑108 (MQ=255) ccAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCtt < 1:557079/108‑1 (MQ=255) aGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGt > 1:672066/1‑139 (MQ=255) tCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTg > 2:853173/1‑139 (MQ=255) cATTTTTTAAAACTAACTTGCCTGGAGTTTACTGCACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCACTTCTGGCAATCGATTTAGGTTg > 2:1204682/1‑138 (MQ=255) aTTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCa < 1:853173/139‑1 (MQ=255) aTTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCa > 2:887484/1‑139 (MQ=255) ttttttAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCa < 2:1198055/139‑1 (MQ=255) tACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAatttc > 2:1280263/1‑139 (MQ=255) tACTGAACCAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAatttc > 2:125026/1‑139 (MQ=255) aaCCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGc > 1:1162750/1‑106 (MQ=255) aaCCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGc < 2:1162750/106‑1 (MQ=255) ccAACTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAatttccatttcc < 1:57882/139‑1 (MQ=255) aCTTATATA‑TTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCgg > 1:1097669/1‑139 (MQ=255) atatatTTTT‑AGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTcc < 2:1029954/138‑1 (MQ=255) atata‑tTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGTTcc < 1:125026/138‑1 (MQ=255) tatatTTTTGAGTACAGCTTGACCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCg > 1:342447/1‑139 (MQ=255) | GTAAACACCGTTACCAGAGCTATTGCCAGAACCTTTTTTTTCATTGCATTTCTTCCCGAGTTGAATTGAGGACGTGATGTCCCAGTGAGTCATTTTTTAAAACTAACTTGCCTGGAGTTTACTGAACCAACTTATATATTTTTGAGTACAGCTTGGCCTCAGCATTACGCCGATGGGTATACACTGTCTTCACACTACAATTTTCAATTCTGGCAATCGATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCG > NZ_CP009273/306339‑306611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |