| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 493,309 | (C)5→4 | intergenic (+34/‑202) | adk → / → hemH | adenylate kinase/ferrochelatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 493,305 | 0 | C | . | 97.7% | 187.4 / ‑1.9 | 44 | intergenic (+30/‑206) | adk/hemH | adenylate kinase/ferrochelatase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (14/29); total (15/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
GGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCGCCCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACC > NZ_CP009273/493168‑493436 | ggCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGGAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCGc < 1:543016/138‑1 (MQ=255)ggCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCGcc < 2:569458/139‑1 (MQ=255)ggCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCGc < 2:295752/138‑1 (MQ=255)ggCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCGc < 1:999818/138‑1 (MQ=255) ctactaCTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCGcccc < 2:1277583/139‑1 (MQ=255) ctactaCTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCGcccc > 1:980681/1‑139 (MQ=255) cAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga < 2:693667/137‑1 (MQ=255) cAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga < 2:336276/137‑1 (MQ=255) cAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga < 2:633241/137‑1 (MQ=255) cAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga < 1:1277058/137‑1 (MQ=255) cAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga < 1:974637/137‑1 (MQ=255) cAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga > 1:56229/1‑137 (MQ=255) cAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga < 1:755667/137‑1 (MQ=255) cAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga < 1:627833/137‑1 (MQ=255) gcagaagcGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga > 1:1119705/1‑130 (MQ=255) gcagaagcGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga < 2:1119705/130‑1 (MQ=255) cagaagcGGGTAATACCGAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTa > 1:468676/1‑137 (MQ=255) cagaagcGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTa < 2:468676/137‑1 (MQ=255) cagaagcGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAgg < 1:1264202/139‑1 (MQ=255) gaagcGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGt < 1:4021/139‑1 (MQ=255) gcGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAg < 2:828198/139‑1 (MQ=255) gcGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAg < 2:18669/139‑1 (MQ=255) tAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGaa < 1:699624/139‑1 (MQ=255) aaTACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga < 2:509006/111‑1 (MQ=255) aaTACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGga > 1:509006/1‑111 (MQ=255) aaTACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAgg < 1:597028/121‑1 (MQ=255) aaTACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAgg > 2:597028/1‑121 (MQ=255) aaTACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCg < 2:980681/139‑1 (MQ=255) aaTACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAAGCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGAAGAGGGTTAGGGTGAGGGGAACAGGCCCg < 2:326475/139‑1 (MQ=255) gACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACtt < 2:1205364/138‑1 (MQ=255) gCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATGCGAAAGCGCGCACGGACAGCCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAg < 1:1272814/139‑1 (MQ=255) aCCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGc > 1:51066/1‑138 (MQ=255) ccAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCaa > 2:921043/1‑139 (MQ=255) ggTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAgg < 2:237673/139‑1 (MQ=255) ttCGCTCTGATCTTGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGAGAGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATattca > 2:1287555/1‑139 (MQ=38) cgcgCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCATCG‑CCCCTCGGGGAGAGGGCTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATattcatt > 2:961555/1‑139 (MQ=255) gcTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAg > 2:1189450/1‑82 (MQ=255) gcTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAg < 1:1189450/82‑1 (MQ=255) aaTCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGgaga < 2:1242467/55‑1 (MQ=255) aaTCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGgaga > 1:1242467/1‑55 (MQ=255) aaTCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCa > 2:71312/1‑89 (MQ=255) aaTCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCa < 1:71312/89‑1 (MQ=255) cTAATTCGAAAGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTg < 2:490532/139‑1 (MQ=255) aaGCGCGCACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTcc < 2:794740/139‑1 (MQ=255) cgcACGGGCAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCttttt < 1:1097042/139‑1 (MQ=255) cgcACGGACAGTCCCCTCGCCCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCtttt > 2:361156/1‑139 (MQ=255) cgcACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCttttt > 1:426776/1‑139 (MQ=255) cgcACGGACAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCttttt < 1:563110/139‑1 (MQ=255) cAGTCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTg < 2:56229/139‑1 (MQ=255) tCCCCTCG‑CCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGAcc > 2:1218947/1‑139 (MQ=255) | GGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACGGACAGTCCCCTCGCCCCCTCGGGGAGAGGGTTAGGGTGAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACC > NZ_CP009273/493168‑493436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |