Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,213,874	Δ1 bp	coding (1091/1524 nt)	pdeG →	cyclic diguanylate phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,213,874	0	G	.	100.0%	103.3 / NA	24	coding (1091/1524 nt)	pdeG	cyclic diguanylate phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/10);  total (14/10)

AACGCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGGATGATTT  >  NZ_CP009273/1213756‑1213994                                                                                                                       |                                                                                                                         aaCGCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCt                                                                                                     >  2:313786/1‑139 (MQ=255)    gCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTgg                                                                                                   >  2:588795/1‑138 (MQ=255)        tCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGt                                                                                               >  2:1142729/1‑138 (MQ=255)             aGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCAcc                                                                                           >  2:977713/1‑137 (MQ=255)             aGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCAcc                                                                                           >  2:277649/1‑137 (MQ=255)                   ttGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGa                                                                                   <  1:641713/139‑1 (MQ=255)                     gCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAg                                                                                  <  1:587630/138‑1 (MQ=255)                                                          tCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGaa                                            >  1:860468/1‑139 (MQ=255)                                                           cGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAg                                                    <  1:53880/130‑1 (MQ=255)                                                           cGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAg                                                    >  2:53880/1‑130 (MQ=255)                                                           cGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGaa                                            >  2:1009826/1‑138 (MQ=255)                                                              atatTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATa                                        <  1:750264/139‑1 (MQ=255)                                                                       cGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATa                                        >  1:275178/1‑130 (MQ=255)                                                                       cGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATa                                        <  2:275178/130‑1 (MQ=255)                                                                       cGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGa                                >  2:67621/1‑138 (MQ=255)                                                                        gCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGaaa                              <  2:521789/139‑1 (MQ=255)                                                                            aCGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAAcc                                                                                                            <  2:1194380/57‑1 (MQ=255)                                                                            aCGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAAcc                                                                                                            >  1:1194380/1‑57 (MQ=255)                                                                            aCGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATgg                          >  2:993808/1‑139 (MQ=255)                                                                            aCGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATgg                          >  2:1118702/1‑139 (MQ=255)                                                                                             tttAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTg          >  1:114205/1‑138 (MQ=255)                                                                                                aaaTTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGgat       <  1:588795/138‑1 (MQ=255)                                                                                                  aTTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGgatgat    <  2:1116399/139‑1 (MQ=255)                                                                                                     tCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGGATGAttt  <  1:365305/138‑1 (MQ=255)                                                                                                                       |                                                                                                                         AACGCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGGATGATTT  >  NZ_CP009273/1213756‑1213994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑