Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,476,2110TG68.1% 69.6 / 43.3 47V357G (GTT→GGT) ynbDphosphatase PAP2/dual specificity phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (8/7);  new base G (16/16);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGGTGTTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGGTTGTTATGT  >  NZ_CP009273/1476076‑1476344
                                                                                                                                       |                                                                                                                                     
ctgGTGTTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTcc                                                                                                                                    >  2:279671/1‑139 (MQ=255)
      tttatttaGGGGCGTTCCCACGACATATCCCGGCACAGAATGCGGTTCTGGACGTCCCCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAag                                                                                                                               <  2:1233004/138‑1 (MQ=255)
            taGGGGCTTTCCCCCGACATATTCCGGCACAGAAGGCGGTTCTGTACGTCCCCTTTGAATTCCCCCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAggggg                                                                                                                         <  1:571022/138‑1 (MQ=255)
                    tttCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGa                                                                                                                 >  2:294780/1‑138 (MQ=255)
                                  tCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATg                                                                                                  >  1:750413/1‑139 (MQ=255)
                                       cacaGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTgg                                                                                              >  2:1145454/1‑138 (MQ=255)
                                            aaTGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACACAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACAt                                                                                        >  2:757121/1‑139 (MQ=255)
                                                cGGTTCTGGCCGTCCCCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTAcg                                                                                    <  1:388869/139‑1 (MQ=255)
                                                  gTTCTGACCGCCTCCTTTGATTCCCCTGGGGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTAcgcg                                                                                  <  2:982568/139‑1 (MQ=255)
                                                      tGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGaa                                                                                >  2:932021/1‑137 (MQ=255)
                                                         aCGTCACCTTTGAATTCCCTCGCGGCCGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCa                                                                           <  2:662871/139‑1 (MQ=255)
                                                             cACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAgg                                                                        >  1:954214/1‑138 (MQ=255)
                                                             cACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAgg                                                                        >  1:1120168/1‑138 (MQ=255)
                                                             cACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAgg                                                                        >  2:1183646/1‑138 (MQ=255)
                                                             cACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAgg                                                                        >  1:781743/1‑138 (MQ=255)
                                                                    gAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGtt                                                                 <  1:257843/138‑1 (MQ=255)
                                                                         cccTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGt                                                            <  1:1183646/138‑1 (MQ=255)
                                                                          ccTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTc                                                           >  1:80754/1‑138 (MQ=255)
                                                                           cTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCa                                                         <  1:279671/139‑1 (MQ=255)
                                                                             cgcgGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCAtt                                                       <  1:865015/139‑1 (MQ=255)
                                                                             cgcgGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCAtt                                                       <  2:985087/139‑1 (MQ=255)
                                                                              gcgGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATtg                                                      <  2:652940/139‑1 (MQ=255)
                                                                                      gCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAggggg                                                                                                                         <  1:472576/64‑1 (MQ=255)
                                                                                      gCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAggggg                                                                                                                         >  2:472576/1‑64 (MQ=255)
                                                                                                aTCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATcgcg                                    <  1:1145454/139‑1 (MQ=255)
                                                                                                aTCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTAcgcg                                                                                  >  1:664691/1‑93 (MQ=255)
                                                                                                aTCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTAcgcg                                                                                  <  2:664691/93‑1 (MQ=255)
                                                                                                aTCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATcgcg                                    <  2:576351/139‑1 (MQ=255)
                                                                                                  cGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATcgcg                                    >  1:80845/1‑137 (MQ=255)
                                                                                                   gACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCa                                  <  2:1010064/138‑1 (MQ=255)
                                                                                                   gACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAg                                 <  2:781743/139‑1 (MQ=255)
                                                                                                    aCTCTATTTTTGTGTACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGt                                >  1:643978/1‑139 (MQ=255)
                                                                                                               gtgtACCGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCtggtggt                     >  1:1040856/1‑139 (MQ=255)
                                                                                                                  tACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCtggtggt                     <  2:153262/136‑1 (MQ=255)
                                                                                                                  tACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCtggtggt                     >  1:153262/1‑136 (MQ=255)
                                                                                                                    ccGATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTggcgg                >  2:507904/1‑139 (MQ=255)
                                                                                                                     cGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTggcggc               <  1:126453/139‑1 (MQ=255)
                                                                                                                      gATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCa              <  1:294780/139‑1 (MQ=255)
                                                                                                                      gATGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCa              <  1:932021/139‑1 (MQ=255)
                                                                                                                       aTGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCa                                                                      <  1:953688/82‑1 (MQ=255)
                                                                                                                       aTGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCa                                                                      >  2:953688/1‑82 (MQ=255)
                                                                                                                       aTGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCAt             >  2:211087/1‑139 (MQ=255)
                                                                                                                       aTGCTGGATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCAt             >  1:14113/1‑139 (MQ=255)
                                                                                                                            ggATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCgcgc                                   <  2:784446/112‑1 (MQ=255)
                                                                                                                            ggATCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCgcgc                                   >  1:784446/1‑112 (MQ=255)
                                                                                                                              aTCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGgttgt       >  2:390287/1‑138 (MQ=255)
                                                                                                                               tCTGGTGGGTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGGTtgtta     >  1:191581/1‑139 (MQ=255)
                                                                                                                                  ggtggtTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTAcgcg                                                                                  >  1:437513/1‑59 (MQ=255)
                                                                                                                                  ggtggtTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTAcgcg                                                                                  <  2:437513/59‑1 (MQ=255)
                                                                                                                                  ggtgggTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGGTtgttatgt  >  1:352024/1‑139 (MQ=255)
                                                                                                                                  ggtgggTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGGTtgttatgt  >  1:388207/1‑139 (MQ=255)
                                                                                                                                  ggtgggTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGGTtgttatgt  >  1:568895/1‑139 (MQ=255)
                                                                                                                                   gtggtTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGgttgtt      <  2:80845/134‑1 (MQ=255)
                                                                                                                                   gtgggTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGgttgtt      <  1:328285/134‑1 (MQ=255)
                                                                                                                                   gtgggTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGgttgtt      <  2:981482/134‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CTGGTGTTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGGAAACATTACGCGAAGAGCAAGGCAGCGTTCTGGTCCATTGTGCATTGGGATTATCGCGCAGTGCGCTGGTGGTGGCGGCATGGTTGTTATGT  >  NZ_CP009273/1476076‑1476344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑