| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 6,672 | G→A | L430F (CTC→TTC) | yaaJ ← | sodium:alanine symporter family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 6,672 | 0 | G | A | 94.3% | 184.0 / 0.9 | 53 | L430F (CTC→TTC) | yaaJ | sodium:alanine symporter family protein |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (27/23); total (29/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
ACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGC > NZ_CP009273/6552‑6807 | aCGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCaa < 2:332929/139‑1 (MQ=255) cgcgTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCaaa > 1:548191/1‑139 (MQ=255) cGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCaaa > 2:920160/1‑133 (MQ=255) cGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCaaa < 1:920160/133‑1 (MQ=255) cGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTTGt > 1:962507/1‑138 (MQ=255) gaAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAg < 2:757250/139‑1 (MQ=255) cTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATg < 2:250188/139‑1 (MQ=255) cGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAgg < 1:72198/139‑1 (MQ=255) atatCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTa < 2:1285867/139‑1 (MQ=255) atatCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTa < 1:1072550/139‑1 (MQ=255) gATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGcc > 2:43307/1‑128 (MQ=255) gATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGcc < 1:43307/128‑1 (MQ=255) aCGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGc > 1:886544/1‑138 (MQ=255) cAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACAt > 1:1286802/1‑139 (MQ=255) ccGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCgg < 1:899968/139‑1 (MQ=255) cGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCgg > 1:968825/1‑138 (MQ=255) cGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGa < 1:84732/139‑1 (MQ=255) gggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACAt > 2:874871/1‑131 (MQ=255) gggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACAt < 1:874871/131‑1 (MQ=255) gggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCgg > 2:34608/1‑137 (MQ=255) gggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCgg > 1:1067159/1‑137 (MQ=255) gggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCgg > 1:733151/1‑137 (MQ=255) gggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCgg > 1:402255/1‑137 (MQ=255) ggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGt > 2:477705/1‑79 (MQ=255) ggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGt < 1:477705/79‑1 (MQ=255) ggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGc > 2:576182/1‑103 (MQ=255) ggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGc < 1:576182/103‑1 (MQ=255) ggCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAg > 2:451585/1‑139 (MQ=255) aGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCa < 1:957721/89‑1 (MQ=255) aGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCa > 2:957721/1‑89 (MQ=255) aGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAgg > 1:170188/1‑139 (MQ=255) ggCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGAt > 2:236456/1‑139 (MQ=255) tggTAGAGAATCACTGGCAATGGTATGAACCACAGGCGAGAAGAGTAAGATGTCAGTCAAATTGGGAATGGCCATGCAGGCCATTATGATGTCTGCCAGTGGCCACATCAGCGGATGGCTTAGCAAGGTGCCGCCGAt < 2:310502/136‑1 (MQ=255) tCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAg < 2:810408/61‑1 (MQ=255) tCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAg > 1:810408/1‑61 (MQ=255) tCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCg > 2:170943/1‑138 (MQ=255) tCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCg > 2:144768/1‑138 (MQ=255) tCACTGGCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAACAAGGTGCCGCCGATGACCGTTGCg > 2:1055918/1‑138 (MQ=255) gCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTg < 2:606874/138‑1 (MQ=255) gCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTg > 1:1281730/1‑138 (MQ=255) gCAATGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTg > 1:748072/1‑138 (MQ=255) aTGGTATGAACCACAGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGa < 1:236499/139‑1 (MQ=255) tGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTg > 2:532996/1‑134 (MQ=255) caGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCcaca > 2:533996/1‑75 (MQ=255) caGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCcaca < 1:533996/75‑1 (MQ=255) caGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCa < 1:667777/132‑1 (MQ=255) caGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCa > 2:667777/1‑132 (MQ=255) aGGCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAacac > 1:754301/1‑136 (MQ=255) gCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGAt < 2:754301/139‑1 (MQ=255) gCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGAt < 2:402255/139‑1 (MQ=255) gCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGAt < 1:34608/139‑1 (MQ=255) gCGAGAACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGAt < 1:451585/139‑1 (MQ=255) gagaACAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCg > 1:1002294/1‑139 (MQ=255) agaaCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGc > 2:705830/1‑139 (MQ=255) | ACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGC > NZ_CP009273/6552‑6807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |