| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 96,834 | A→C | Q235P (CAA→CCA) | murG → | undecaprenyldiphospho‑muramoylpentapeptide beta‑N‑acetylglucosaminyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 96,834 | 0 | A | C | 93.8% | 160.5 / 3.4 | 48 | Q235P (CAA→CCA) | murG | undecaprenyldiphospho‑muramoylpentapeptide beta‑N‑acetylglucosaminyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base C (23/22); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
GTTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTT > NZ_CP009273/96699‑96963 | gtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCAcc > 1:1040766/1‑139 (MQ=255)gtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCAcc < 1:204384/139‑1 (MQ=255) ttCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCAcc < 2:269548/138‑1 (MQ=255) ttCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCAcc > 1:269548/1‑138 (MQ=255) ttCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCg < 2:1042852/139‑1 (MQ=255) aGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCgcagca > 1:716863/1‑139 (MQ=255) gACAATGCCGCAGGTTGCTGCGAAACTAGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATa > 1:750502/1‑119 (MQ=255) gACAATGCCGCAGGTTGCTGCGAAACTAGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATa < 2:750502/119‑1 (MQ=255) aCAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATtgat > 1:331299/1‑138 (MQ=255) aCAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTATtgat > 1:1145024/1‑138 (MQ=255) aTGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTATTGATGata < 1:689545/139‑1 (MQ=255) tGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGatat > 1:295187/1‑139 (MQ=255) cAGGTTGCTGCGAAACTGGGTTATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCTTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATggcgg < 2:625724/139‑1 (MQ=255) cAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATggcgg < 1:173478/139‑1 (MQ=255) cAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATggcgg < 1:624391/139‑1 (MQ=255) ttCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATgtc > 2:95221/1‑139 (MQ=255) ttCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATgtc > 2:403748/1‑139 (MQ=255) ttCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATgtc > 1:728233/1‑139 (MQ=255) cTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTg < 1:849387/139‑1 (MQ=255) tCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCg > 2:572052/1‑139 (MQ=255) tCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCg > 1:1091764/1‑139 (MQ=255) gCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCAc > 1:353601/1‑62 (MQ=255) gCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCAc < 2:353601/62‑1 (MQ=255) aTCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCgg < 1:1093027/139‑1 (MQ=255) aTCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCgg < 2:1040766/139‑1 (MQ=255) cAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATtgat > 1:450197/1‑87 (MQ=255) cAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATtgat < 2:450197/87‑1 (MQ=255) cAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATtga > 1:193132/1‑86 (MQ=255) cAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATtga < 2:193132/86‑1 (MQ=255) agagCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTg < 1:490305/138‑1 (MQ=255) agCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAAt < 1:432939/76‑1 (MQ=255) agCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAAt > 2:432939/1‑76 (MQ=255) ggCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTaa < 1:95221/139‑1 (MQ=255) gTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGGGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACggggcg > 2:690268/1‑135 (MQ=255) aTCCGTTGAACAGGCGTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATcgcc > 1:208624/1‑139 (MQ=255) tATGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCgg > 1:873200/1‑139 (MQ=255) tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATcgc > 1:753660/1‑120 (MQ=255) tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATcgc < 2:753660/120‑1 (MQ=255) tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGc > 2:482841/1‑126 (MQ=255) tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGc < 1:482841/126‑1 (MQ=255) tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGc < 1:23766/138‑1 (MQ=255) tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCg < 2:1234090/139‑1 (MQ=255) tGCCGAAGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCg > 1:1234090/1‑139 (MQ=255) aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCgg < 2:363104/91‑1 (MQ=255) aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCgg > 1:363104/1‑91 (MQ=255) aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTAc > 2:1032281/1‑129 (MQ=255) aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTAc < 1:1032281/129‑1 (MQ=255) aaGCGGGGCCACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCgttgtt > 2:1250296/1‑139 (MQ=255) | GTTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGCGTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTT > NZ_CP009273/96699‑96963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |