| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,405,453 | C→T | P251P (CCG→CCA) | ttcA ← | tRNA 2‑thiocytidine(32) synthetase TtcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,405,453 | 0 | C | T | 84.8% | 122.0 / 12.4 | 46 | P251P (CCG→CCA) | ttcA | tRNA 2‑thiocytidine(32) synthetase TtcA |
| Reads supporting (aligned to +/- strand): ref base C (3/4); new base T (20/19); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01 | |||||||||||
CTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGTTGCACGGAATAATC > NZ_CP009273/1405320‑1405588 | cttcttctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGcac > 2:499390/1‑139 (MQ=255) cttctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCGCCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGcacc < 2:117191/137‑1 (MQ=255) ccAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCa < 1:1025015/137‑1 (MQ=255) ccAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATc > 2:888654/1‑139 (MQ=255) ggATCTCTTCGCGATCAAACGCTAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGAt < 1:1178629/139‑1 (MQ=255) ggATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGAt < 1:132997/139‑1 (MQ=255) tctTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGcc > 2:414404/1‑139 (MQ=255) tCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGATATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTg > 2:25757/1‑139 (MQ=255) cgcgATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTg > 1:1070438/1‑138 (MQ=255) cGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCaa < 1:64946/139‑1 (MQ=255) aCCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGt < 2:1035264/139‑1 (MQ=255) ccACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGca < 2:131231/66‑1 (MQ=255) ccACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGca > 1:131231/1‑66 (MQ=255) aCCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCAcc < 1:876818/139‑1 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGcacc < 2:829658/54‑1 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGcacc > 1:829658/1‑54 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGcc > 1:515147/1‑91 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGcc < 2:515147/91‑1 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGtt > 2:239847/1‑103 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGtt < 1:239847/103‑1 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGAc > 2:1275177/1‑139 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGAc > 2:776244/1‑139 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCa > 2:1140467/1‑129 (MQ=255) tGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCa < 1:1140467/129‑1 (MQ=255) gTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGtt < 2:1011965/139‑1 (MQ=255) tGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCa < 2:1245537/107‑1 (MQ=255) tGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCa > 1:1245537/1‑107 (MQ=255) ccTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAgg < 2:938662/138‑1 (MQ=255) tCGAACAGGTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCtgtg < 2:1070438/139‑1 (MQ=255) gTTGGTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGcac < 1:291900/139‑1 (MQ=255) gTATCGCACAGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAgg < 1:888654/138‑1 (MQ=255) cacaGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGt > 2:712095/1‑44 (MQ=255) cacaGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGt < 1:712095/44‑1 (MQ=255) cacaGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGAt > 1:921508/1‑61 (MQ=255) cacaGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGAt < 2:921508/61‑1 (MQ=255) cacaGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGc < 1:476818/81‑1 (MQ=255) cacaGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGc > 2:476818/1‑81 (MQ=255) cacaGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGTTGCACg > 1:319822/1‑139 (MQ=255) cacaGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGTTGCACg > 2:192051/1‑139 (MQ=255) cacaGATGCGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGTTGCACg > 2:1080095/1‑139 (MQ=255) cacaGATGCGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGTTGCa < 1:1057682/137‑1 (MQ=255) cacaGATGCGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGTTGCa > 2:1057682/1‑137 (MQ=255) aTGCGATGGCACCACATTCTGCATCGTGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGTTGCACGGaat > 2:9720/1‑138 (MQ=255) aTGCGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGAt < 2:1245701/45‑1 (MQ=255) aTGCGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGAt > 1:1245701/1‑45 (MQ=255) cGATGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGTTGCACGGAATAATc > 1:1278048/1‑139 (MQ=255) | CTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCACATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCCAGTCACGCAACATGTCAGCAATCACCTGACGTTGCAGGTTAGGCTGTGAACCGCACAGGTTGCACGGAATAATC > NZ_CP009273/1405320‑1405588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |