Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,412,718	A→C	intergenic (‑232/‑210)	kilR ← / → sieB	killing protein KilR/superinfection exclusion B family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,412,718	0	A	C	100.0%	121.0 / NA	32	intergenic (‑232/‑210)	kilR/sieB	killing protein KilR/superinfection exclusion B family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/18);  total (14/18)

CCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGT  >  NZ_CP009273/1412582‑1412855                                                                                                                                         |                                                                                                                                          ccaAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTaccca                                                                                                                                         <  1:1030571/139‑1 (MQ=255)     aCCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTaccca                                                                                                                                         >  1:1061944/1‑135 (MQ=255)     aCCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTaccca                                                                                                                                         <  2:1061944/135‑1 (MQ=255)          tAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGt                                                                                                                                >  1:318958/1‑139 (MQ=255)            aGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCg                                                                                                                              <  2:713258/139‑1 (MQ=255)            aGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCg                                                                                                                              <  2:551857/139‑1 (MQ=255)                   aCATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTAttt                                                                                                                        <  1:1066143/138‑1 (MQ=255)                             tAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAgg                                                                                                             >  1:311634/1‑139 (MQ=255)                                   tgCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCa                                                                                                                                    <  2:506016/110‑1 (MQ=255)                                   tgCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCa                                                                                                                                    >  1:506016/1‑110 (MQ=255)                                   tgCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTa                                                                                                                       >  2:808512/1‑123 (MQ=255)                                   tgCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTa                                                                                                                       <  1:808512/123‑1 (MQ=255)                                   tgCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACt                                                                                                       >  1:321253/1‑139 (MQ=255)                                    gCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTc                                                                                                      >  1:653134/1‑139 (MQ=255)                                                 aGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCatttaaat                                                                                         >  2:282154/1‑139 (MQ=255)                                                       aTTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCatttaaatttaa                                                                                     >  2:3734/1‑137 (MQ=255)                                                       aTTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCatttaaatttaa                                                                                     >  2:1143437/1‑137 (MQ=255)                                                             cGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGAc                                                                                >  2:252543/1‑136 (MQ=255)                                                                 tCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCg                                                                         <  2:653134/139‑1 (MQ=255)                                                                   acaAAGTTCACTTTAAAAAGGGCAGCCAACAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGAc                                                                       <  2:318958/139‑1 (MQ=255)                                                                   acaAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGa                                                                        <  1:1162934/138‑1 (MQ=255)                                                                   acaAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGa                                                                        <  2:267730/138‑1 (MQ=255)                                                                   acaAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGa                                                                        <  1:600541/138‑1 (MQ=255)                                                                   acaAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGa                                                                        <  1:263214/138‑1 (MQ=255)                                                                        aTTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCttt                                                                  <  2:321253/139‑1 (MQ=255)                                                                         ttCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTa                                                                 >  1:641937/1‑139 (MQ=255)                                                                            aCTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAg                                                                             >  1:632418/1‑124 (MQ=255)                                                                            aCTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAg                                                                             <  2:632418/124‑1 (MQ=255)                                                                            aCTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATg                                                               <  2:1178994/138‑1 (MQ=255)                                                                            aCTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATg                                                               <  1:731181/138‑1 (MQ=255)                                                                                 aaaaaGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGatat                                                          <  1:1143437/138‑1 (MQ=255)                                                                                      gggCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGATATCGGaaa                                                    >  1:253561/1‑139 (MQ=255)                                                                                        gCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGATATCGGAAATg                                                  <  1:3734/139‑1 (MQ=255)                                                                                                        cTTCAAGAAAAAAACTGATGCCGCCAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGc                                  >  2:959187/1‑139 (MQ=255)                                                                                                                            ccgccAGGACTACCCACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCTCCACCt              <  2:667686/139‑1 (MQ=255)                                                                                                                                        cacacaGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCggt  >  2:148430/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                          CCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTTAAATTTAACAGACAAGACCGACTCTTTATGGATATCGGAAATGCGCCTTCGTGTTGTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGT  >  NZ_CP009273/1412582‑1412855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑