| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,506,942 | T→G | G344G (GGT→GGG) | ydcS → | ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,506,942 | 0 | T | G | 98.4% | 231.6 / ‑3.5 | 64 | G344G (GGT→GGG) | ydcS | ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (32/31); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
CAAATGGATGAACTGGTCATTAACGCCAAAAGTGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTT > NZ_CP009273/1506810‑1507073 | cAAATGGATGAACTGGTCATTAACGCCAAAAGTGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTaa < 1:591840/138‑1 (MQ=255) aTGAACTGGTCATTAACGCCAAAAGTGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTc > 1:980726/1‑138 (MQ=255) ggTCATTAACGCCAAAAGTGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAAt > 1:392629/1‑139 (MQ=255) gTCATTAACGCCAAAAGTGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATc > 1:1064443/1‑139 (MQ=255) ccAAAAGTGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTgg > 1:1112129/1‑138 (MQ=255) ccAAAAGTGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTgg > 1:1148823/1‑138 (MQ=255) aaaaGTGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGaaa < 1:919394/139‑1 (MQ=255) aaGTGCAGGGCGATGTGGCGGCCTGGTTTGGTTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAAc < 1:898113/139‑1 (MQ=255) tGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGcc < 2:980726/139‑1 (MQ=255) tGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGcc < 2:1112129/139‑1 (MQ=255) gggCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCta > 1:326106/1‑137 (MQ=255) tgtgGCGGCCTGGTTTGGCTCGTTACCGTGAGTGCCGGAGGGGGGTAAAGCCAGTCCGTTATTAAGCGAAAAAGGATGTGAAACCAACGGGGTTAACTATTTCGACAAAATCGCCTTCTGGAAAGCGGCTATAGCAg > 1:181448/1‑137 (MQ=255) tgtgGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGaa < 2:1148823/139‑1 (MQ=255) tgtgGCGGCCTGGTTTGGCTCGTTAACGGCAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGCAAAAATCGCCTTCTGGAAAACGACCATAGCAg > 1:1203978/1‑137 (MQ=255) cggcCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAggggg < 1:136585/139‑1 (MQ=255) gcCTGGTTTGGCTCGTTACCGGTAGTGCCGGGAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGc > 2:154921/1‑138 (MQ=255) tGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGt > 2:974776/1‑139 (MQ=255) tttGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTg < 1:974776/139‑1 (MQ=255) ggCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGtt < 2:1064443/138‑1 (MQ=255) gCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTc < 2:327444/138‑1 (MQ=255) cTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAGCTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTccc > 2:555034/1‑139 (MQ=255) gTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGTAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTa > 2:1204352/1‑138 (MQ=255) ttACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTa > 1:824694/1‑137 (MQ=255) ttACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTa > 2:837643/1‑137 (MQ=255) gTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCt < 2:270408/139‑1 (MQ=255) tAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTg > 2:551170/1‑139 (MQ=255) tGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGAc > 2:262175/1‑139 (MQ=255) tGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGAc > 1:877411/1‑139 (MQ=255) ccGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACg < 2:212729/138‑1 (MQ=255) ccGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACg < 1:262175/138‑1 (MQ=255) gAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGcc < 1:805631/92‑1 (MQ=255) gAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGcc > 2:805631/1‑92 (MQ=255) gAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAg > 1:1192615/1‑138 (MQ=255) aaGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGa < 1:227312/139‑1 (MQ=255) aGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGAt > 1:1188219/1‑139 (MQ=255) gtgtAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTAc < 2:1192615/139‑1 (MQ=255) aaGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTg < 1:277396/138‑1 (MQ=255) aGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGcc < 2:522763/139‑1 (MQ=255) gCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCa < 1:916609/139‑1 (MQ=255) gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGttt < 1:1052321/98‑1 (MQ=255) gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTg > 1:288606/1‑99 (MQ=255) gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTg < 2:288606/99‑1 (MQ=255) gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACg > 2:851199/1‑119 (MQ=255) gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACg < 1:851199/119‑1 (MQ=255) gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTAt > 1:979528/1‑139 (MQ=255) gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTAt > 1:858926/1‑139 (MQ=255) gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTAt > 1:530019/1‑139 (MQ=255) gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGACTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGttt > 2:1052321/1‑98 (MQ=255) attaGGCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGt > 2:1133676/1‑139 (MQ=255) ggCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACg > 1:847547/1‑108 (MQ=255) ggCGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACg < 2:847547/108‑1 (MQ=255) cGAAAAAGGTTGTGAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTaa < 2:1024428/139‑1 (MQ=255) tgtgAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTggg > 2:344978/1‑139 (MQ=255) gtgAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTgggg > 2:1081594/1‑139 (MQ=255) gtgAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTggg < 1:741834/138‑1 (MQ=255) gtgAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTggg < 2:588738/138‑1 (MQ=255) gtgAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTggg < 2:824694/138‑1 (MQ=255) tgAAACCAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTgggg < 2:835541/138‑1 (MQ=255) ccAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCttt < 2:530019/139‑1 (MQ=255) ccAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCttt < 1:253937/139‑1 (MQ=255) cAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTg > 2:492414/1‑101 (MQ=255) cAACGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTg < 1:492414/101‑1 (MQ=255) aCGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATggg < 1:897959/109‑1 (MQ=255) aCGGGTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATggg > 2:897959/1‑109 (MQ=255) | CAAATGGATGAACTGGTCATTAACGCCAAAAGTGCAGGGCGATGTGGCGGCCTGGTTTGGCTCGTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTT > NZ_CP009273/1506810‑1507073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |