Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,513,670 G→A V129V (GTG→GTA)  curA → NADPH‑dependent curcumin/dihydrocurcumin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,513,6700GA100.0% 226.1 / NA 60V129V (GTG→GTA) curANADPH‑dependent curcumin/dihydrocurcumin reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/33);  total (27/33)

GAATCATCCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAA  >  NZ_CP009273/1513538‑1513802
                                                                                                                                    |                                                                                                                                    
gAATCATCCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAgg                                                                                                                                 >  1:354398/1‑138 (MQ=255)
                cAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCg                                                                                                                >  2:830082/1‑139 (MQ=255)
                cAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCg                                                                                                                >  1:878102/1‑139 (MQ=255)
                  gTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCt                                                                                                              >  1:1123181/1‑139 (MQ=255)
                       gCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTtata                                                                                                          <  1:695516/138‑1 (MQ=255)
                       gCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTatat                                                                                                         <  1:881169/139‑1 (MQ=255)
                       gCGACTGGGTGATGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTtat                                                                                                           <  2:539868/137‑1 (MQ=255)
                           cTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGc                                                                                                     <  1:600340/139‑1 (MQ=255)
                           cTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGc                                                                                                     <  1:499393/139‑1 (MQ=255)
                               gTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTAc                                                                                                 <  2:1123181/139‑1 (MQ=255)
                                tGCTGGGGTACAGTGGATGGCCAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCCTCCGGCAAATCCATCCTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACt                                                                                                <  1:686662/139‑1 (MQ=255)
                                        tACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCg                                                                                        >  1:1269159/1‑139 (MQ=255)
                                        tACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCg                                                                                        >  1:1079348/1‑139 (MQ=255)
                                          cAGTGGATGGCAAGTCTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCTGt                                                                                      <  1:740066/139‑1 (MQ=255)
                                          cAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCgg                                                                                       <  1:333115/138‑1 (MQ=255)
                                          cAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCgg                                                                                       <  2:501871/138‑1 (MQ=255)
                                           tgttgATTGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTc                                                                                     <  2:702371/135‑1 (MQ=255)
                                               gATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCgg                                                                                       >  2:115442/1‑133 (MQ=255)
                                               gATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCgg                                                                                       >  2:674781/1‑133 (MQ=255)
                                               gATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCgg                                                                                       <  1:674781/133‑1 (MQ=255)
                                               gATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCgg                                                                                       <  1:115442/133‑1 (MQ=255)
                                               gATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGcc                                                                                 >  1:984387/1‑139 (MQ=255)
                                               gATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGcc                                                                                 >  1:893162/1‑139 (MQ=255)
                                                       gACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCg                                                                                                                <  1:1148587/100‑1 (MQ=255)
                                                       gACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCg                                                                                                                >  2:1148587/1‑100 (MQ=255)
                                                            tGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGa                                                                     <  2:1079348/138‑1 (MQ=255)
                                                               cATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACg                                                                 <  2:508644/139‑1 (MQ=255)
                                                                atatCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAAGCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGTGCCTACTGCATATCGGTCAGCCTAAAGAGGGCGAAACGt                                                                <  2:31979/139‑1 (MQ=255)
                                                                  atCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTa                                                                                                                   <  1:837526/86‑1 (MQ=255)
                                                                  atCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTa                                                                                                                   >  2:837526/1‑86 (MQ=255)
                                                                  atCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTtg                                                              >  1:179890/1‑139 (MQ=255)
                                                                  atCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTtg                                                              >  2:875307/1‑139 (MQ=255)
                                                                    ccAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTtggt                                                            >  2:1171877/1‑139 (MQ=255)
                                                                        tggtgATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTtggtgg                                                          >  2:983709/1‑137 (MQ=255)
                                                                          gtgATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGc                                                      >  2:963136/1‑139 (MQ=255)
                                                                             atgatCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGc                                                                                                     >  1:243809/1‑89 (MQ=255)
                                                                             atgatCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGc                                                                                                     <  2:243809/89‑1 (MQ=255)
                                                                             atgatCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGt                                                                                      <  1:742312/104‑1 (MQ=255)
                                                                             atgatCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGt                                                                                      >  2:742312/1‑104 (MQ=255)
                                                                             atgatCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTgc                                                   >  1:1270182/1‑139 (MQ=255)
                                                                              tgatCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTgcg                                                  <  1:816234/139‑1 (MQ=255)
                                                                                  cTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGt                                                                                      <  2:147877/99‑1 (MQ=255)
                                                                                  cTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGt                                                                                      >  1:147877/1‑99 (MQ=255)
                                                                                    ggTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAg                                                                                                                                  >  2:1290393/1‑53 (MQ=255)
                                                                                    ggTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAg                                                                                                                                  <  1:1290393/53‑1 (MQ=255)
                                                                                    ggTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCa                                                                                                                          >  2:878094/1‑61 (MQ=255)
                                                                                    ggTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCa                                                                                                                          <  1:878094/61‑1 (MQ=255)
                                                                                    ggTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTc                                                                                     <  2:951546/98‑1 (MQ=255)
                                                                                    ggTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTc                                                                                     >  1:951546/1‑98 (MQ=255)
                                                                                     gTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCgg                                                                                       <  2:1291122/95‑1 (MQ=255)
                                                                                     gTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGt                                                                                      >  1:1168887/1‑96 (MQ=255)
                                                                                     gTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGt                                                                                      <  2:1168887/96‑1 (MQ=255)
                                                                                     gTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGAc                                             <  1:1240881/137‑1 (MQ=255)
                                                                                     gTGAAACTTGGCGATCATCAGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCgg                                                                                       >  1:1291122/1‑95 (MQ=255)
                                                                                      tGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTatat                                                                                                         <  2:733051/76‑1 (MQ=255)
                                                                                      tGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTatat                                                                                                         >  1:733051/1‑76 (MQ=255)
                                                                                              ggCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTg                                   <  2:984387/138‑1 (MQ=255)
                                                                                                   tcatcCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCg                             <  1:239330/139‑1 (MQ=255)
                                                                                                     atcCGCAAAATCCATCGTGGTCGCTGGGTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGa                            <  1:1183658/138‑1 (MQ=255)
                                                                                                                               ggTGTACTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTaaa  <  2:1269159/138‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GAATCATCCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAA  >  NZ_CP009273/1513538‑1513802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑