Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,547,080 G→A intergenic (‑141/+5) yddM ← / ← adhP HigA family addiction module antitoxin/alcohol dehydrogenase AdhP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,547,0800GA98.6% 261.0 / ‑4.4 69intergenic (‑141/+5)yddM/adhPHigA family addiction module antitoxin/alcohol dehydrogenase AdhP
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (41/27);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

TTACTCTCAGTAGCGTTCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGGCCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGCACCACTTTACCTTCGGCGGCAAACTGGA  >  NZ_CP009273/1546948‑1547215
                                                                                                                                    |                                                                                                                                       
ttACTCTCAGTAGCGTTCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACctct                                                                                                                                    >  1:33268/1‑138 (MQ=255)
   ctctCAGTAGCGTTCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAg                                                                                                                                 <  2:767228/138‑1 (MQ=255)
     ctcAGTAGCGTTCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGa                                                                                                                              >  1:320006/1‑139 (MQ=255)
      tcAGTAGCGTTCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGAc                                                                                                                             >  2:39060/1‑139 (MQ=255)
        aGTAGCGTTCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACgg                                                                                                                           <  2:257461/139‑1 (MQ=255)
             cGTTCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACgg                                                                                                                           <  2:238670/134‑1 (MQ=255)
             cGTTCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACgg                                                                                                                           >  1:238670/1‑134 (MQ=255)
               ttCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAaatcaa                                                                                                                    >  1:222109/1‑139 (MQ=255)
                  cTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACgg                                                                                                                           <  2:1176902/129‑1 (MQ=255)
                  cTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACgg                                                                                                                           >  1:1176902/1‑129 (MQ=255)
                  cTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAatcaatca                                                                                                                 >  2:993704/1‑139 (MQ=255)
                  cTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAatcaatca                                                                                                                 >  2:634127/1‑139 (MQ=255)
                          ggAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCgg                                                                                                         >  2:743080/1‑139 (MQ=255)
                                 tATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGa                                                                                                  >  2:333030/1‑139 (MQ=255)
                                  aTCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGAt                                                                                                 >  2:1191842/1‑139 (MQ=255)
                                        attGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGcc                                                                                           >  1:713597/1‑139 (MQ=255)
                                         ttGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCct                                                                                          <  2:713597/139‑1 (MQ=255)
                                           gTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAaatcaat                                                                                                                   <  1:844617/112‑1 (MQ=255)
                                           gTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAaatcaat                                                                                                                   >  2:844617/1‑112 (MQ=255)
                                           gTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCcttc                                                                                        >  2:1062681/1‑139 (MQ=255)
                                           gTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCcttc                                                                                        >  2:893493/1‑139 (MQ=255)
                                           gTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCcttc                                                                                        >  1:1271694/1‑139 (MQ=255)
                                               cttcctACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCttcttc                                                                                     <  1:444061/138‑1 (MQ=255)
                                               cttcctACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCttcttc                                                                                     <  1:1062681/138‑1 (MQ=255)
                                                 tcctACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAt                                                                                  >  2:334664/1‑139 (MQ=255)
                                                 tcctACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAt                                                                                  >  2:1134098/1‑139 (MQ=255)
                                                 tcctACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAt                                                                                  >  2:741628/1‑139 (MQ=255)
                                                  cctACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAt                                                                                  <  1:1154319/138‑1 (MQ=255)
                                                   ctACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAtct                                                                                >  1:1056360/1‑139 (MQ=255)
                                                      cGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTa                                                                                                                                  >  2:810396/1‑86 (MQ=255)
                                                      cGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTa                                                                                                                                  <  1:810396/86‑1 (MQ=255)
                                                         aacaaTAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTaa                                                                          <  1:333030/139‑1 (MQ=255)
                                                           caaTAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATTGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACAATGCGGCCACGGATTTTGCCTTCTTCCAt                                                                                  >  2:1103035/1‑129 (MQ=255)
                                                           caaTAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAt                                                                                  <  1:1103035/129‑1 (MQ=255)
                                                           caaTAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTa                                                                           >  1:395433/1‑136 (MQ=255)
                                                            aataatCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGa                                                                       <  2:320006/139‑1 (MQ=255)
                                                                      ttAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGAt                                                             >  2:1072394/1‑139 (MQ=255)
                                                                       tAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGAt                                                             <  2:1056360/138‑1 (MQ=255)
                                                                       tAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATg                                                            >  1:272936/1‑139 (MQ=255)
                                                                                       gagaGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACg                                              >  1:1092232/1‑137 (MQ=255)
                                                                                          agTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGa                                                                       >  1:183353/1‑109 (MQ=255)
                                                                                          agTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGa                                                                       <  2:183353/109‑1 (MQ=255)
                                                                                            tgtgCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGc                                        >  2:518728/1‑138 (MQ=255)
                                                                                            tgtgCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGAGATGATGTCCGCNAANg                                                  >  2:183551/1‑128 (MQ=255)
                                                                                            tgtgCGATGCTGCGACCCGAACATAGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGc                                        >  2:176075/1‑138 (MQ=255)
                                                                                              tgCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGAc                                     >  1:897849/1‑139 (MQ=255)
                                                                                                cGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACtt                                   <  2:837546/139‑1 (MQ=255)
                                                                                                     ctgcGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGc                              <  1:993704/139‑1 (MQ=255)
                                                                                                      tgcGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAtct                                                                                >  2:260519/1‑88 (MQ=255)
                                                                                                      tgcGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAtct                                                                                <  1:260519/88‑1 (MQ=255)
                                                                                                      tgcGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCg                                                        <  2:1253498/112‑1 (MQ=255)
                                                                                                      tgcGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCg                                                        >  1:1253498/1‑112 (MQ=255)
                                                                                                      tgcGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGca                             >  1:779090/1‑139 (MQ=255)
                                                                                                      tgcGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGca                             >  1:240517/1‑139 (MQ=255)
                                                                                                      tgcGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGc                              <  1:635950/138‑1 (MQ=255)
                                                                                                      tgcGACCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGc                              >  2:635950/1‑138 (MQ=255)
                                                                                                          aCCCGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGcaccac                         >  2:319123/1‑139 (MQ=255)
                                                                                                             cGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATg                                                                                                            <  1:956160/53‑1 (MQ=255)
                                                                                                             cGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATg                                                                                                            >  2:956160/1‑53 (MQ=255)
                                                                                                             cGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCgg                               <  2:256487/130‑1 (MQ=255)
                                                                                                             cGAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCgg                               >  1:256487/1‑130 (MQ=255)
                                                                                                              gAACATGGCAGTCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGCACCACTTTa                     <  2:897849/139‑1 (MQ=255)
                                                                                                                         tCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAt                                                                                  <  2:546290/67‑1 (MQ=255)
                                                                                                                         tCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCAt                                                                                  >  1:546290/1‑67 (MQ=255)
                                                                                                                         tCGCAGCAAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGCACCACTTTACCTTcggcgg           >  2:1041292/1‑138 (MQ=255)
                                                                                                                             agcaAAGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGCACCACTTTACCTTCGGCGGCAAAc      <  1:1041292/139‑1 (MQ=255)
                                                                                                                                 aaGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGCACCACTTTACCTTCGGCGGCAAACTGGa  <  2:395433/139‑1 (MQ=255)
                                                                                                                                 aaGACCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGCACCACTTTACCTTCGGCGGCAAACTGGa  <  1:334664/139‑1 (MQ=255)
                                                                                                                                  aGGCCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGCACCACTTTACCTTCGGCGGCAAACTgg   >  2:702598/1‑137 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TTACTCTCAGTAGCGTTCCTTCACTGGGAACACTATCATTATTGTTCCTTCCTACGCAACAATAATCCATTTAGCCATTTCATGTTGGAGAGTGTGCGATGCTGCGACCCGAACATGGCAGTCGCAGCAAAGGCCTCTTAGTGACGGAAATCAATCACCATGCGGCCACGGATTTTGCCTTCTTCCATCTCAGTAAAGATGGTGTTGATGTCCGCTAACGGACGCAGGGCGACTTTCGGCACCACTTTACCTTCGGCGGCAAACTGGA  >  NZ_CP009273/1546948‑1547215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑