Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,549,065	C→T	A288T (GCG→ACG)	maeA ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,549,065	0	C	T	100.0%	209.9 / NA	54	A288T (GCG→ACG)	maeA	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/29);  total (25/29)

TCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAAC  >  NZ_CP009273/1548936‑1549195                                                                                                                                  |                                                                                                                                   tCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTtgc                                                                                                                           >  2:1148072/1‑139 (MQ=255)  cTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCggctggct                                                                                                                          >  2:974261/1‑139 (MQ=255)       gcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGAt                                                                                                                     >  1:987690/1‑139 (MQ=255)          ccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAg                                                                                                                  <  1:498197/139‑1 (MQ=255)                 cTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCg                                                                                                           <  1:1148072/139‑1 (MQ=255)                        aaTCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGt                                                                                                      <  2:918979/137‑1 (MQ=255)                              tCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGc                                                                                                                               >  2:262065/1‑106 (MQ=255)                              tCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGc                                                                                                                               <  1:262065/106‑1 (MQ=255)                              tCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCACATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccgccg                                                                                              >  1:1047344/1‑139 (MQ=255)                               cgcgCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGc                                                                                                                               >  2:851440/1‑105 (MQ=255)                               cgcgCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGc                                                                                                                               <  1:851440/105‑1 (MQ=255)                                gcgcTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                            <  1:696281/139‑1 (MQ=255)                                gcgcTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGGGTGCCGACTGTTACCGCCGCa                                                                                            <  1:569497/139‑1 (MQ=255)                                 cgcTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAg                                                                                           <  2:929707/139‑1 (MQ=255)                                    tGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGc                                                                                        >  2:200570/1‑139 (MQ=255)                                      ggTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                            >  1:9421/1‑133 (MQ=255)                                      ggTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                            <  2:9421/133‑1 (MQ=255)                                       gTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCt                                                                                     <  1:26364/139‑1 (MQ=255)                                            ggAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATg                                                                                >  1:460936/1‑139 (MQ=255)                                                atgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCAt                                                                            >  1:840824/1‑139 (MQ=255)                                                atgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCAt                                                                            >  1:533370/1‑139 (MQ=255)                                                 tgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGc                                                                                                                               <  2:1251396/87‑1 (MQ=255)                                                 tgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGc                                                                                                                               >  1:1251396/1‑87 (MQ=255)                                                 tgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTg                                                                                         <  2:797062/125‑1 (MQ=255)                                                 tgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTg                                                                                         >  1:797062/1‑125 (MQ=255)                                                 tgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATc                                                                           >  1:314652/1‑139 (MQ=255)                                                  gatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCg                                                                          >  1:600849/1‑139 (MQ=255)                                                  gatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCg                                                                          >  2:642547/1‑139 (MQ=255)                                                  gatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCg                                                                          >  2:1103340/1‑139 (MQ=255)                                                        ttCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTaa                                                                      >  1:1200793/1‑137 (MQ=255)                                                         tCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTGTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTaaaa                                                                    <  2:141420/138‑1 (MQ=255)                                                            gCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAAc                                                                <  2:981628/139‑1 (MQ=255)                                                            gCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAAc                                                                <  2:314652/139‑1 (MQ=255)                                                                tGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAt                                                            <  1:1191489/139‑1 (MQ=255)                                                                  ccGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGGCCACCTGCCGTGCGGCTTGCTGCGATCATTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                                          <  2:600849/139‑1 (MQ=255)                                                                  ccGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                                          <  1:647265/139‑1 (MQ=255)                                                                  ccGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                                          <  2:533370/139‑1 (MQ=255)                                                                           gCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                                 <  1:101054/139‑1 (MQ=255)                                                                           gCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                                 <  2:987690/139‑1 (MQ=255)                                                                               aaCCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAg                                               <  2:1200793/137‑1 (MQ=255)                                                                                aCCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCgg                                            <  2:72602/139‑1 (MQ=255)                                                                                    gcgcCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGtt                                          >  2:562496/1‑137 (MQ=255)                                                                                    gcgcCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGtt                                          >  2:744373/1‑137 (MQ=255)                                                                                           ggAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACgg                                 >  2:285287/1‑139 (MQ=255)                                                                                           ggAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACgg                                 >  2:1156092/1‑139 (MQ=255)                                                                                               gACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGTTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTaa                                    <  2:1151168/132‑1 (MQ=255)                                                                                               gACGATTTTTTTCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTaa                                    >  1:1151168/1‑132 (MQ=255)                                                                                                        tttCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAttttt                     <  2:873968/138‑1 (MQ=255)                                                                                                        tttCTCGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAttttt                     <  1:785147/138‑1 (MQ=255)                                                                                                          tctcGCTTAACTGACCACCTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAttttttt                   >  2:391616/1‑138 (MQ=255)                                                                                                                       accaccTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCa     <  2:979862/139‑1 (MQ=255)                                                                                                                          accTGCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAAc  <  1:200570/139‑1 (MQ=255)                                                                                                                              gCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                                 >  2:272553/1‑88 (MQ=255)                                                                                                                              gCCGTGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                                 <  1:272553/88‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   TCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAAC  >  NZ_CP009273/1548936‑1549195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑