Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,550,159 C→T R21H (CGC→CAC)  sra ← stationary‑phase‑induced ribosome‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,550,1590CT98.0% 182.2 / ‑4.3 50R21H (CGC→CAC) srastationary‑phase‑induced ribosome‑associated protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (25/24);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

AAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACGTGCTAAACCCCT  >  NZ_CP009273/1550026‑1550292
                                                                                                                                     |                                                                                                                                     
aaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTgg                                                                                                                                  >  2:582967/1‑139 (MQ=255)
    acCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAg                                                                                                                              <  1:1086709/139‑1 (MQ=255)
           cgcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGt                                                                                                                                 <  1:758707/129‑1 (MQ=255)
           cgcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGt                                                                                                                                 >  2:758707/1‑129 (MQ=255)
            gcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTa                                                                                                                      <  2:97390/139‑1 (MQ=255)
                gCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgt                                                                                                                  <  2:845845/139‑1 (MQ=255)
                 cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                                 <  2:1039620/139‑1 (MQ=255)
                 cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                                 <  2:965904/139‑1 (MQ=255)
                 cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                                 <  2:505378/139‑1 (MQ=255)
                 cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                                 <  1:492182/139‑1 (MQ=255)
                 cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                                 <  1:582967/139‑1 (MQ=255)
                     ggTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagt                                                                                                             >  1:1142048/1‑139 (MQ=255)
                          ttAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcc                                                                                                           >  2:1058981/1‑136 (MQ=255)
                          ttAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                                 <  2:140434/130‑1 (MQ=255)
                          ttAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtc                                                                                                                 >  1:140434/1‑130 (MQ=255)
                                gTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAatat                                                                                                   >  2:1135726/1‑138 (MQ=255)
                                 tCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGa                                                                                                 >  2:698148/1‑139 (MQ=255)
                                 tCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGa                                                                                                 >  1:114468/1‑139 (MQ=255)
                                 tCATTGAGGACTGATGGGTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGa                                                                                                 >  2:203996/1‑139 (MQ=255)
                                   aTTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACg                                                                                               >  1:813013/1‑139 (MQ=255)
                                            tGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACg                                                                                      >  1:1020528/1‑139 (MQ=255)
                                              aTGGTTATGAATTACTTTTCAGCGGGGAGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGt                                                                                    >  1:340929/1‑139 (MQ=255)
                                                    aTGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGAtt                                                                              >  2:405378/1‑139 (MQ=255)
                                                      gAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGAttt                                                                             >  1:1278662/1‑138 (MQ=255)
                                                            cTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCAtttt                                                                       >  2:781375/1‑138 (MQ=255)
                                                            cTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCAtttt                                                                       >  2:818637/1‑138 (MQ=255)
                                                               ttCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcca                                                                                                          <  2:7566/100‑1 (MQ=255)
                                                               ttCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGgtccagtcca                                                                                                          >  1:7566/1‑100 (MQ=255)
                                                                  aGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAActcct                                                                <  2:635126/139‑1 (MQ=255)
                                                                  aGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAActcct                                                                <  1:1058981/139‑1 (MQ=255)
                                                                  aGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAActcct                                                                <  1:158573/139‑1 (MQ=255)
                                                                  aGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAActcct                                                                <  1:725010/139‑1 (MQ=255)
                                                                        gCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATcc                                                          <  1:781375/139‑1 (MQ=255)
                                                                                              tttACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGacaa                                     <  1:178069/138‑1 (MQ=255)
                                                                                                tactacGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACAt                                  >  2:69655/1‑139 (MQ=255)
                                                                                                     cGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCg                             >  1:199127/1‑139 (MQ=255)
                                                                                                     cGCTGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCg                             >  1:422647/1‑139 (MQ=255)
                                                                                                        tGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTaa                                                                     >  2:213921/1‑96 (MQ=255)
                                                                                                        tGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTaa                                                                     <  1:213921/96‑1 (MQ=255)
                                                                                                        tGGATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAg                          >  1:834996/1‑139 (MQ=255)
                                                                                                          gATTTGTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAg                          <  2:813013/137‑1 (MQ=255)
                                                                                                               gTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTaa                                                                     >  1:645721/1‑89 (MQ=255)
                                                                                                               gTCACCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGGCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTaa                                                                     <  2:645721/89‑1 (MQ=255)
                                                                                                                  aCCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAaca                <  2:1233073/139‑1 (MQ=255)
                                                                                                                  aCCTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAac                 <  1:69655/138‑1 (MQ=255)
                                                                                                                    cTTCGGTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACg              >  1:578026/1‑139 (MQ=255)
                                                                                                                         gTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACGTGCTa         <  2:1020528/139‑1 (MQ=255)
                                                                                                                         gTAACTATTTTGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACGTGCTa         <  2:1045530/139‑1 (MQ=255)
                                                                                                                                ttttGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACGTGCTAAAccc    <  2:885767/137‑1 (MQ=255)
                                                                                                                                 tttGTGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACGTGCTAAACCCCt  >  1:719922/1‑138 (MQ=255)
                                                                                                                                     |                                                                                                                                     
AAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCCGTAGCGAAGAAACACGTGCTAAACCCCT  >  NZ_CP009273/1550026‑1550292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑