Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	100,067	A→G	G142G (GGA→GGG)	ftsQ →	cell division protein FtsQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	100,067	0	A	G	100.0%	189.5 / NA	53	G142G (GGA→GGG)	ftsQ	cell division protein FtsQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/20);  total (33/20)

AACAACGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTA  >  NZ_CP009273/99940‑100200                                                                                                                                |                                                                                                                                      aacaacGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAg                                                                                                                            <  2:239188/139‑1 (MQ=255)    aacGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGt                                                                                                                         <  2:996840/139‑1 (MQ=255)    aacGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGt                                                                                                                         <  1:904025/139‑1 (MQ=255)     acGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTg                                                                                                                        >  2:213699/1‑139 (MQ=255)     acGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTg                                                                                                                        >  1:111878/1‑139 (MQ=255)     acGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTg                                                                                                                        >  1:977263/1‑139 (MQ=255)           gCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTgccgc                                                                                                                    >  2:1036727/1‑137 (MQ=255)                          ggTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCagcaag                                                                                                   <  1:890656/139‑1 (MQ=255)                           gTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCagcaag                                                                                                   >  1:1196516/1‑138 (MQ=255)                               gCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                              <  1:213699/139‑1 (MQ=255)                               gCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                              <  2:727861/139‑1 (MQ=255)                                cGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTg                                                                                             >  1:856335/1‑139 (MQ=255)                                      aaaGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCa                                                                                       <  1:788317/139‑1 (MQ=255)                                       aaGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCaa                                                                                      <  1:1030302/139‑1 (MQ=255)                                         gCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATg                                                                                    <  1:839671/139‑1 (MQ=255)                                             tGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGTAAGGGAATACCTTCAGCGTGCCGCCAGaa                                                                                                               <  1:1066508/108‑1 (MQ=255)                                             tGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGaa                                                                                                               >  2:1066508/1‑108 (MQ=255)                                             tGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTc                                                                                         >  1:613138/1‑130 (MQ=255)                                             tGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTc                                                                                         <  2:613138/130‑1 (MQ=255)                                             tGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTg                                                                                 >  1:622013/1‑138 (MQ=255)                                             tGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTg                                                                                 >  1:1117090/1‑138 (MQ=255)                                               gCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTa                                                                               <  1:949240/138‑1 (MQ=255)                                                    atgaATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                              >  2:494248/1‑118 (MQ=255)                                                    atgaATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                              <  1:494248/118‑1 (MQ=255)                                                                   aTCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCaa                                                          >  1:469028/1‑139 (MQ=255)                                                                               atGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCa                                              <  2:59467/139‑1 (MQ=255)                                                                                   gCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGt                                                                                <  2:897524/101‑1 (MQ=255)                                                                                   gCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGt                                                                                >  1:897524/1‑101 (MQ=255)                                                                                   gCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTg                                                <  2:673379/133‑1 (MQ=255)                                                                                   gCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTg                                                >  1:673379/1‑133 (MQ=255)                                                                                      gATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTAt                                       >  2:385208/1‑139 (MQ=255)                                                                                       aTTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATc                                      >  1:540746/1‑139 (MQ=255)                                                                                       aTTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATc                                      >  2:104256/1‑139 (MQ=255)                                                                                                       aTCAACATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGAt                      >  2:454543/1‑139 (MQ=255)                                                                                                          aaCATATGGTAGACGCGGAAGGGAATACCTTCCGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCt                   >  1:1110463/1‑139 (MQ=255)                                                                                                          aaCATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCt                   >  1:316455/1‑139 (MQ=255)                                                                                                          aaCATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCt                   >  1:780376/1‑139 (MQ=255)                                                                                                          aaCATATGGTAGACGCGGAAGGGAATACCTTCAGCGTCCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCt                   >  1:660266/1‑139 (MQ=255)                                                                                                            cATATGGTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTgg                 >  1:1227375/1‑139 (MQ=255)                                                                                                                 ggTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACg                                                                                                             <  1:1089520/42‑1 (MQ=255)                                                                                                                 ggTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACg                                                                                                             >  2:1089520/1‑42 (MQ=255)                                                                                                                 ggTAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAg            >  1:44841/1‑139 (MQ=255)                                                                                                                   tAGACGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa          <  1:1182133/139‑1 (MQ=255)                                                                                                                      aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                              >  2:616116/1‑52 (MQ=255)                                                                                                                      aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGt                                                                                              <  1:616116/52‑1 (MQ=255)                                                                                                                      aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa          >  2:1182133/1‑136 (MQ=255)                                                                                                                      aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa          >  2:219872/1‑136 (MQ=255)                                                                                                                      aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa          >  2:280226/1‑136 (MQ=255)                                                                                                                      aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa          >  1:44023/1‑136 (MQ=255)                                                                                                                      aCGCGGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa          >  1:834300/1‑136 (MQ=255)                                                                                                                      aCGCGGAAGGGAATACCTTCAGCGTGCAGCCAGAACGCACAAACAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGGAATGGGGCAGATGCTGGCAAAGGa          >  2:391050/1‑136 (MQ=255)                                                                                                                       cgcgGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGa          >  2:1069826/1‑135 (MQ=255)                                                                                                                        gcgGAAGGGAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGAt     <  2:111878/139‑1 (MQ=255)                                                                                                                           gaagggaATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTa  >  1:608933/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                      AACAACGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAGATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATGCTGGCAAAGGACAGATTTA  >  NZ_CP009273/99940‑100200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑