Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,581,105	A→G	V296A (GTC→GCC)	ydeQ ←	fimbrial protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,581,105	0	A	G	100.0%	206.1 / NA	54	V296A (GTC→GCC)	ydeQ	fimbrial protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

TGCGCGGATTTTCTTACAGGTGTAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGACTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACG  >  NZ_CP009273/1580977‑1581238                                                                                                                                 |                                                                                                                                      tgCGCGGATTTTCTTACAGGTGTAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGa                                                                                                                              <  2:733390/138‑1 (MQ=255) tgCGCGGATTTTCTTACAGGTGTAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGa                                                                                                                              <  2:1182105/138‑1 (MQ=255) tgCGCGGATTTTCTTACAGGTGTAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGa                                                                                                                              <  2:1169812/138‑1 (MQ=255) tgCGCGGATTTTCTTACAGGTGTAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGAc                                                                                                                             >  1:724972/1‑139 (MQ=255)                      gtAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGtt                                                                                                        >  1:276112/1‑139 (MQ=255)                       tAGGCTAATAGCCTTCACAAAATAATTCTCCGCGAAATTTTCGTGCATGGCGCGCATAGACTACGGTCAAAATCACAATTATTAATAGCTAAAAGTGACACCACTGGCTGACTGGACGGTTCCGGCTGAAACCTTGttt                                                                                                       >  2:1226214/1‑139 (MQ=255)                       tAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGttt                                                                                                       >  1:519234/1‑139 (MQ=255)                        aGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTc                                                                                                      >  2:88255/1‑139 (MQ=255)                              aTAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGa                                                                                                                              >  1:1053745/1‑109 (MQ=255)                              aTAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGa                                                                                                                              <  2:710123/109‑1 (MQ=255)                              aTAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGa                                                                                                                              <  2:1053745/109‑1 (MQ=255)                              aTAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGa                                                                                                                              >  1:710123/1‑109 (MQ=255)                              aTAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGttt                                                                                                >  2:545798/1‑139 (MQ=255)                              aTAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGttt                                                                                                >  1:1289150/1‑139 (MQ=255)                              aTAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGttt                                                                                                >  1:753879/1‑139 (MQ=255)                               tAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTg                                                                                               >  1:1114250/1‑139 (MQ=255)                               tAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTg                                                                                               <  1:1163677/139‑1 (MQ=255)                                  ccTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTcc                                                                                            <  2:654707/139‑1 (MQ=255)                                    ttCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCa                                                                                           >  1:1034104/1‑138 (MQ=255)                                    ttCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCa                                                                                           >  2:520121/1‑138 (MQ=255)                                    ttCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCa                                                                                           >  1:973449/1‑138 (MQ=255)                                        caAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGt                                                                                      <  1:520121/139‑1 (MQ=255)                                          aaaTAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTg                                                                                    <  2:973449/139‑1 (MQ=255)                                                   tCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAAc                                                                           >  2:1174315/1‑139 (MQ=255)                                                   tCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAAc                                                                           >  2:259314/1‑139 (MQ=255)                                                      gcgcAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCa                                                                        <  1:1174315/139‑1 (MQ=255)                                                                cGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACttt                                                              >  2:1154686/1‑139 (MQ=255)                                                                    aaTGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCt                                                           <  1:541696/138‑1 (MQ=255)                                                                      tGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCtttt                                                        <  2:1034104/139‑1 (MQ=255)                                                                         aGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTtttatt                                                     >  2:541488/1‑139 (MQ=255)                                                                             cATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACt                                                 <  1:3829/139‑1 (MQ=255)                                                                                   cTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGtt                                                                                     <  1:488563/97‑1 (MQ=255)                                                                                   cTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGtt                                                                                     >  2:488563/1‑97 (MQ=255)                                                                                   cTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTccc                                            >  1:256728/1‑138 (MQ=255)                                                                                      aGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAg                                         <  1:541488/138‑1 (MQ=255)                                                                                       ggTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAGCCCAGAGGTACTTTGCt                                                           <  1:350739/119‑1 (MQ=255)                                                                                       ggTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCt                                                           >  2:350739/1‑119 (MQ=255)                                                                                       ggTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTtttat                                                      <  2:541902/124‑1 (MQ=255)                                                                                       ggTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTtttat                                                      >  1:541902/1‑124 (MQ=255)                                                                                           aaaaTCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGAc                                   <  1:545798/139‑1 (MQ=255)                                                                                            aaaTCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACa                                  >  1:24306/1‑139 (MQ=255)                                                                                            aaaTCACAATTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACa                                  >  1:1102356/1‑139 (MQ=255)                                                                                                   aaTTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTa                                                                  >  2:178736/1‑100 (MQ=255)                                                                                                   aaTTATTCATAGATAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTa                                                                  <  1:178736/100‑1 (MQ=255)                                                                                                               aTAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGt                                                                                      >  2:371591/1‑68 (MQ=255)                                                                                                               aTAAAAGTGACACCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGt                                                                                      <  1:371591/68‑1 (MQ=255)                                                                                                                         cacCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCAtt     <  2:519234/139‑1 (MQ=255)                                                                                                                         cacCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCAtt     <  2:1114250/139‑1 (MQ=255)                                                                                                                         cacCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCAtt     <  2:672542/139‑1 (MQ=255)                                                                                                                         cacCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCAtt     <  2:339404/139‑1 (MQ=255)                                                                                                                         cacCAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCAt      >  2:312099/1‑138 (MQ=255)                                                                                                                           ccAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGt                                                                                                  >  2:436448/1‑44 (MQ=255)                                                                                                                           ccAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGt                                                                                                  <  1:436448/44‑1 (MQ=255)                                                                                                                            cAATGGCTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACg  <  2:483613/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      TGCGCGGATTTTCTTACAGGTGTAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAATCACAATTATTCATAGATAAAAGTGACACCAATGACTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACG  >  NZ_CP009273/1580977‑1581238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑