Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,603,097	A→C	G61G (GGT→GGG)	yneE ←	bestrophin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,603,097	0	A	C	100.0%	154.1 / NA	46	G61G (GGT→GGG)	yneE	bestrophin family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/28);  total (18/28)

TCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAG  >  NZ_CP009273/1602963‑1603226                                                                                                                                       |                                                                                                                                  tCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCga                                                                                                                               <  2:880407/139‑1 (MQ=255) tCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCga                                                                                                                               <  2:232593/139‑1 (MQ=255)      cAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATg                                                                                                                          >  1:299447/1‑139 (MQ=255)           tCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  1:83263/139‑1 (MQ=255)           tCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  2:616070/139‑1 (MQ=255)           tCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  1:188832/139‑1 (MQ=255)           tCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  2:159817/139‑1 (MQ=255)                   aCCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGc                                                                                                               >  2:646862/1‑137 (MQ=255)                         cgcAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     >  1:177183/1‑125 (MQ=255)                         cgcAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  2:177183/125‑1 (MQ=255)                                aCGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGa                                                                                                 <  1:124778/138‑1 (MQ=255)                                     cGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCa                                                                                           <  1:287749/139‑1 (MQ=255)                                      gTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAg                                                                                          <  1:86669/139‑1 (MQ=255)                                        gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATg                                                                                                                          >  1:770956/1‑105 (MQ=255)                                        gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATg                                                                                                                          <  2:770956/105‑1 (MQ=255)                                        gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGAt                                                                                        >  1:411889/1‑139 (MQ=255)                                           gCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  2:411889/139‑1 (MQ=255)                                              atcatcAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCgtg                                                                                   >  1:1135556/1‑138 (MQ=255)                                              atcatcAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCgtg                                                                                   >  2:1066502/1‑138 (MQ=255)                                                      cTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgc                                                                          <  2:1079850/139‑1 (MQ=255)                                                         cccccAAAGTTTTCGCGCTTCAACGTATCGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcag                                                                        >  1:476989/1‑138 (MQ=255)                                                         cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAGCCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAg                                                                                                            <  2:757462/102‑1 (MQ=255)                                                         cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGa                                                                                                             <  2:286882/101‑1 (MQ=255)                                                         cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGa                                                                                                             >  1:286882/1‑101 (MQ=255)                                                         cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAg                                                                                                            >  1:757462/1‑102 (MQ=255)                                                         cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  1:557554/125‑1 (MQ=255)                                                         cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     >  2:557554/1‑125 (MQ=255)                                                         cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcag                                                                        >  1:916396/1‑138 (MQ=255)                                                         cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcag                                                                        >  1:823822/1‑138 (MQ=255)                                                           cccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCAt                                                                     >  1:774563/1‑139 (MQ=255)                                                           cccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCAt                                                                     <  2:1135556/139‑1 (MQ=255)                                                                    ttCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATaa                                                             <  1:137086/138‑1 (MQ=255)                                                                                cGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGaaaa                                                <  1:1066502/139‑1 (MQ=255)                                                                                     cGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAgaga                                            >  2:155591/1‑138 (MQ=255)                                                                                             cccGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGag                                    <  2:1116680/138‑1 (MQ=255)                                                                                                            aaaaCCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGa                     >  1:931254/1‑138 (MQ=255)                                                                                                               aCCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  2:299447/139‑1 (MQ=255)                                                                                                               aCCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  2:163218/139‑1 (MQ=255)                                                                                                                   aGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGaaa                                                 >  2:189865/1‑103 (MQ=255)                                                                                                                   aGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGaaa                                                 <  1:189865/103‑1 (MQ=255)                                                                                                                   aGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTg              >  1:305864/1‑138 (MQ=255)                                                                                                                    gAAAAATGGCGATGGCGTCCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  2:181842/139‑1 (MQ=255)                                                                                                                    gAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:173216/139‑1 (MQ=255)                                                                                                                    gAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:155591/139‑1 (MQ=255)                                                                                                                    gAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGAGGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  2:476989/139‑1 (MQ=255)                                                                                                                              gatggcgaCCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAg  <  2:773820/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  TCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAG  >  NZ_CP009273/1602963‑1603226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑