Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,603,103	A→G	I59I (ATT→ATC)	yneE ←	bestrophin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,603,103	0	A	G	100.0%	163.0 / NA	46	I59I (ATT→ATC)	yneE	bestrophin family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/27);  total (19/27)

CAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAG  >  NZ_CP009273/1602968‑1603226                                                                                                                                        |                                                                                                                            cAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATg                                                                                                                          >  1:299447/1‑139 (MQ=255)      tCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  2:159817/139‑1 (MQ=255)      tCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  1:188832/139‑1 (MQ=255)      tCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  2:616070/139‑1 (MQ=255)      tCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  1:83263/139‑1 (MQ=255)              aCCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGc                                                                                                               >  2:646862/1‑137 (MQ=255)                    cgcAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     >  1:177183/1‑125 (MQ=255)                    cgcAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGaa                                                                                                                     <  2:177183/125‑1 (MQ=255)                           aCGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGa                                                                                                 <  1:124778/138‑1 (MQ=255)                                cGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCa                                                                                           <  1:287749/139‑1 (MQ=255)                                 gTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAg                                                                                          <  1:86669/139‑1 (MQ=255)                                   gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATg                                                                                                                          <  2:770956/105‑1 (MQ=255)                                   gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATg                                                                                                                          >  1:770956/1‑105 (MQ=255)                                   gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGAt                                                                                        >  1:411889/1‑139 (MQ=255)                                      gCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  2:411889/139‑1 (MQ=255)                                         atcatcAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCgtg                                                                                   >  2:1066502/1‑138 (MQ=255)                                         atcatcAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCgtg                                                                                   >  1:1135556/1‑138 (MQ=255)                                                 cTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgc                                                                          <  2:1079850/139‑1 (MQ=255)                                                    cccccAAAGTTTTCGCGCTTCAACGTATCGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcag                                                                        >  1:476989/1‑138 (MQ=255)                                                    cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAGCCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAg                                                                                                            <  2:757462/102‑1 (MQ=255)                                                    cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGa                                                                                                             <  2:286882/101‑1 (MQ=255)                                                    cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGa                                                                                                             >  1:286882/1‑101 (MQ=255)                                                    cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAg                                                                                                            >  1:757462/1‑102 (MQ=255)                                                    cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  1:557554/125‑1 (MQ=255)                                                    cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     >  2:557554/1‑125 (MQ=255)                                                    cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcag                                                                        >  1:916396/1‑138 (MQ=255)                                                    cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcag                                                                        >  1:823822/1‑138 (MQ=255)                                                      cccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCAt                                                                     <  2:1135556/139‑1 (MQ=255)                                                      cccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCAt                                                                     >  1:774563/1‑139 (MQ=255)                                                               ttCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATaa                                                             <  1:137086/138‑1 (MQ=255)                                                                           cGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGaaaa                                                <  1:1066502/139‑1 (MQ=255)                                                                                cGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAgaga                                            >  2:155591/1‑138 (MQ=255)                                                                                        cccGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGag                                    <  2:1116680/138‑1 (MQ=255)                                                                                                       aaaaCCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGa                     >  1:931254/1‑138 (MQ=255)                                                                                                          aCCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  2:299447/139‑1 (MQ=255)                                                                                                          aCCAAGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  2:163218/139‑1 (MQ=255)                                                                                                              aGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGaaa                                                 >  2:189865/1‑103 (MQ=255)                                                                                                              aGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGaaa                                                 <  1:189865/103‑1 (MQ=255)                                                                                                              aGAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTg              >  1:305864/1‑138 (MQ=255)                                                                                                               gAAAAATGGCGATGGCGTCCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  2:181842/139‑1 (MQ=255)                                                                                                               gAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:173216/139‑1 (MQ=255)                                                                                                               gAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:155591/139‑1 (MQ=255)                                                                                                               gAAAAATGGCGATGGCGACCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGAGGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  2:476989/139‑1 (MQ=255)                                                                                                                         gatggcgaCCCCGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAg  <  2:773820/139‑1 (MQ=255)                                                                                                                                 ccccGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:829926/119‑1 (MQ=255)                                                                                                                                 ccccGAGGATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            >  2:829926/3‑121 (MQ=255)                                                                                                                                        |                                                                                                                            CAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAG  >  NZ_CP009273/1602968‑1603226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑