Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,635,187 T→C P42P (CCT→CCC)  ynfU → putative zinc‑binding protein YnfU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,635,1870TC96.4% 91.4 / ‑3.4 28P42P (CCT→CCC) ynfUputative zinc‑binding protein YnfU
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (13/14);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

GAAAAGCCTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTGT  >  NZ_CP009273/1635053‑1635322
                                                                                                                                      |                                                                                                                                       
gAAAAGCCTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCAtt                                                                                                                                     >  2:1025694/1‑139 (MQ=255)
      ccTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGcaaca                                                                                                                               <  2:251189/139‑1 (MQ=255)
      ccTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGcaaca                                                                                                                               <  1:598465/139‑1 (MQ=255)
          tGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACaaa                                                                                                                             <  2:838508/137‑1 (MQ=255)
               gAACGTAAGAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTc                                                                                                                       >  2:646927/1‑138 (MQ=255)
                  cGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGa                                                                                                                   >  2:1239377/1‑139 (MQ=255)
                     aaaaaCTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACa                                                                                                                 >  2:814635/1‑138 (MQ=255)
                             aaaaTCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTa                                                                                                          <  1:782503/137‑1 (MQ=255)
                             aaaaTCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTa                                                                                                          <  2:1274792/137‑1 (MQ=255)
                                aTCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCt                                                                                                     <  1:1044296/139‑1 (MQ=255)
                                     gCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGc                                                                                                <  1:1025694/139‑1 (MQ=255)
                                               tctcGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCtgattgat                                                                                       <  2:341268/138‑1 (MQ=255)
                                                   gTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTttat                                                                                  >  1:1087539/1‑139 (MQ=255)
                                                   gTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTttat                                                                                  >  1:286005/1‑139 (MQ=255)
                                                             ccTGCTCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACaa                                                                         <  2:439111/138‑1 (MQ=255)
                                                                           aCCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATAttt                                                          >  2:180836/1‑139 (MQ=255)
                                                                            cccAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATAttt                                                          >  2:440922/1‑138 (MQ=255)
                                                                               aaGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACa                                                                                                                 <  1:527864/80‑1 (MQ=255)
                                                                               aaGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACa                                                                                                                 >  2:527864/1‑80 (MQ=255)
                                                                               aaGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTa                                                                                                          <  2:102320/87‑1 (MQ=255)
                                                                               aaGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTa                                                                                                          >  1:102320/1‑87 (MQ=255)
                                                                                    tCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAACGCTGTAGCCTGATTGATTTTATTAGt                                                                              >  2:921792/1‑110 (MQ=255)
                                                                                    tCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGt                                                                              <  1:921792/110‑1 (MQ=255)
                                                                                                                      cccTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAGCCCGCCTGcgcg               >  2:104759/1‑139 (MQ=255)
                                                                                                                      cccTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGc                <  2:286005/138‑1 (MQ=255)
                                                                                                                      cccTTTTGATCTGCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGc                <  1:870373/138‑1 (MQ=255)
                                                                                                                                  gCCCCCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATtg   >  2:780116/1‑139 (MQ=255)
                                                                                                                                   ccccCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCtgat                                                                                           >  1:791223/1‑50 (MQ=255)
                                                                                                                                   ccccCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCtgat                                                                                           <  2:791223/50‑1 (MQ=255)
                                                                                                                                   ccccCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATtgt  >  2:600979/1‑139 (MQ=255)
                                                                                                                                   ccccCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATtgt  >  2:1169626/1‑139 (MQ=255)
                                                                                                                                   ccccCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATtgt  >  2:958206/1‑139 (MQ=255)
                                                                                                                                   cccTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATtgt  >  2:938807/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GAAAAGCCTATGTCAGAACGTAAAAACTCAAAATCACGCCGTAATTATCTCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTGT  >  NZ_CP009273/1635053‑1635322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑