| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 103,795 | G→A | G251D (GGT→GAT) | lpxC → | UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 103,795 | 0 | G | A | 95.6% | 238.1 / 0.6 | 68 | G251D (GGT→GAT) | lpxC | UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (31/34); total (32/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01 | |||||||||||
TGTGCCTGGGCGGCAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGGTCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCG > NZ_CP009273/103660‑103925 | tgtgCCTGGGCGGCAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATca > 1:1278634/1‑139 (MQ=255) gtgCCTGGGCGGCAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATca < 2:572497/138‑1 (MQ=255) tgCCTGGGCGGCAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATcac > 1:865948/1‑138 (MQ=255) tgCCTGGGCGGCAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATcac > 1:411632/1‑138 (MQ=255) ggcggcAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAAtatta > 1:394838/1‑139 (MQ=255) ggcggcAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAAtatta > 2:1170379/1‑139 (MQ=255) ggcggcAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAAtatta > 2:1276356/1‑139 (MQ=255) ggcggcAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAAtatta > 2:352256/1‑139 (MQ=255) gcAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTgg > 2:930657/1‑139 (MQ=255) gcAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTgg > 1:234562/1‑139 (MQ=255) tCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCAtt > 1:776977/1‑139 (MQ=255) tCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCAtt > 2:325494/1‑139 (MQ=255) ttGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTAcc < 2:501030/139‑1 (MQ=255) tgCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCt > 2:1267012/1‑139 (MQ=255) ttgttgACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATc < 2:407886/139‑1 (MQ=255) gttgACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTAcc > 1:677804/1‑126 (MQ=255) gttgACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTAcc < 2:677804/126‑1 (MQ=255) aCGATTATCACAGACTGATCGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCACGATGCAATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTc < 2:227128/138‑1 (MQ=255) ttATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCa < 2:865948/139‑1 (MQ=255) ttATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCa > 1:396858/1‑139 (MQ=255) ttATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCa < 2:234562/139‑1 (MQ=255) ttATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCAGGTCATGCa < 2:510683/139‑1 (MQ=255) tCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATc > 1:585446/1‑126 (MQ=255) tCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATc < 2:585446/126‑1 (MQ=255) tCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACt > 1:1248363/1‑138 (MQ=255) tACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATg > 1:640455/1‑129 (MQ=255) tACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATg < 2:640455/129‑1 (MQ=255) tACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCa > 2:1185051/1‑131 (MQ=255) tACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCa < 1:1185051/131‑1 (MQ=255) cTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATcac < 2:340681/87‑1 (MQ=255) cTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATcac > 1:340681/1‑87 (MQ=255) aaCGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGGTCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAAc > 2:746919/1‑139 (MQ=255) aaCGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCg > 1:769389/1‑118 (MQ=255) aaCGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCg < 2:769389/118‑1 (MQ=255) aaCGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACaaa < 1:263654/138‑1 (MQ=255) aaCGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAAc > 1:427666/1‑139 (MQ=255) aaCGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAAc > 1:810053/1‑139 (MQ=255) aaCGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAAc > 2:172860/1‑139 (MQ=255) aCGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCa < 1:352256/139‑1 (MQ=255) ggCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGGTCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAgg < 1:746919/139‑1 (MQ=255) ggCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAgg < 1:1071376/139‑1 (MQ=255) gCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATc < 1:208023/106‑1 (MQ=255) gCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATc > 2:208023/1‑106 (MQ=255) gCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATaa < 2:1195136/124‑1 (MQ=255) gCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATaa > 1:1195136/1‑124 (MQ=255) gCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGc > 1:1231737/1‑139 (MQ=255) aCGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGaaa > 1:1135218/1‑137 (MQ=255) tgtgCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc < 1:1108635/138‑1 (MQ=255) tgtgCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc < 2:123900/138‑1 (MQ=255) tgtgCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc < 2:1231737/138‑1 (MQ=255) tgtgCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc < 1:930657/138‑1 (MQ=255) tgtgCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc < 1:925888/138‑1 (MQ=255) tgtgCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc < 1:696351/138‑1 (MQ=255) cGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTggg < 2:776977/139‑1 (MQ=255) cacaAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTgg < 1:935879/135‑1 (MQ=255) cacaAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTgg > 2:935879/1‑135 (MQ=255) aTGCTCGATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGa < 1:1276356/139‑1 (MQ=255) gATGCGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTc < 2:335407/138‑1 (MQ=255) cGATCGGTGACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGga < 2:394838/139‑1 (MQ=255) ggTGACTTGTTCATGTGTGGTCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGacgacg < 1:181698/139‑1 (MQ=255) gACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATAtgtg > 1:91406/1‑120 (MQ=255) gACTTGTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATAtgtg < 2:91406/120‑1 (MQ=255) gTTCATGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAAc < 2:344434/137‑1 (MQ=255) aTGTGTGATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCg < 1:968102/138‑1 (MQ=255) gtgtgATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTc < 1:945731/47‑1 (MQ=255) gtgtgATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTc > 2:945731/1‑47 (MQ=255) gtgtgATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGa < 2:614907/110‑1 (MQ=255) gtgtgATCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGa > 1:614907/1‑110 (MQ=255) | TGTGCCTGGGCGGCAGCTTCGATTGTGCCATCGTTGTTGACGATTATCGCGTACTGAACGAAGACGGCCTGCGTTTTGAAGACGAATTTGTGCGTCACAAAATGCTCGATGCGATCGGTGACTTGTTCATGTGTGGTCACAATATTATTGGTGCATTTACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCG > NZ_CP009273/103660‑103925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |