Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,718,009	A→G	E544E (GAA→GAG)	ydhK →	FUSC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,718,009	0	A	G	100.0%	236.7 / NA	64	E544E (GAA→GAG)	ydhK	FUSC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/34);  total (30/34)

GTCGCGTTGGCGTGGTTAGCGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAATTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTGCGC  >  NZ_CP009273/1717875‑1718135                                                                                                                                       |                                                                                                                               ggcgcgTTGGCGTGGCTAGCGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTg                                                                                                                            <  2:339396/137‑1 (MQ=255)         ggCGTGGTTAGCGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCt                                                                                                                     <  1:585955/138‑1 (MQ=255)          gCGTGGTTAGCGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCa                                                                                                                   >  2:818909/1‑139 (MQ=255)                    cGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTAtcatca                                                                                                         >  1:1240438/1‑139 (MQ=255)                         gCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACgt                                                                                                      >  1:1057387/1‑137 (MQ=255)                         gCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACgt                                                                                                      >  2:267801/1‑137 (MQ=255)                           cATTCTGCGTCCAGGATCGGATGCTTGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCAGCACgtcagt                                                                                                  <  2:883087/139‑1 (MQ=255)                                  cGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGtt                                                                                             <  2:1273661/137‑1 (MQ=255)                                              gATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAg                                                                                >  1:617866/1‑138 (MQ=255)                                              gATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAg                                                                                >  1:528187/1‑138 (MQ=255)                                                    cGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACt                                                                                                                 <  1:1105248/99‑1 (MQ=255)                                                    cGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACt                                                                                                                 >  2:1105248/1‑99 (MQ=255)                                                         aggcctccgccATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCt                                                                    <  1:124377/137‑1 (MQ=255)                                                              gccgccATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCg                                                               <  2:1217713/139‑1 (MQ=255)                                                               ccgccATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGt                                                              >  1:970882/1‑139 (MQ=255)                                                                cgccCTATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGtt                                                             <  1:809977/139‑1 (MQ=255)                                                                cgccATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGtt                                                             <  2:676609/139‑1 (MQ=255)                                                                          gcgcgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACg                                                   <  1:1201683/139‑1 (MQ=255)                                                                          gcgcgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACg                                                   <  2:265685/139‑1 (MQ=255)                                                                          gcgcgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACg                                                   <  1:696314/139‑1 (MQ=255)                                                                          gcgcgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACg                                                   <  2:846263/139‑1 (MQ=255)                                                                             cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGtt                                                                                             >  2:204542/1‑94 (MQ=255)                                                                             cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGtt                                                                                             <  1:204542/94‑1 (MQ=255)                                                                             cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTg                                                >  1:68163/1‑139 (MQ=255)                                                                             cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTg                                                <  1:1031192/139‑1 (MQ=255)                                                                             cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTg                                                <  2:1163911/139‑1 (MQ=255)                                                                             cgcTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTg                                                <  1:671261/139‑1 (MQ=255)                                                                                     cGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAg                                        <  2:68163/139‑1 (MQ=255)                                                                                               tCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGaa                              >  1:551446/1‑139 (MQ=255)                                                                                               tCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGaa                              <  2:617866/139‑1 (MQ=255)                                                                                               tCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCACTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGaa                              <  2:548615/139‑1 (MQ=255)                                                                                                 gATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAAc                             <  2:1240438/138‑1 (MQ=255)                                                                                                  aTCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTg                           <  2:80182/139‑1 (MQ=255)                                                                                                         aaGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCAGTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTTGCCGTTGGTACTTACGCTGGGGTGCAGTGCTGCTGAATTGTTCTCAt                    >  2:242132/1‑139 (MQ=255)                                                                                                          aGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGtgctg                                   >  1:249624/1‑123 (MQ=255)                                                                                                          aGCCGCCATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGtgctg                                   <  2:249624/123‑1 (MQ=255)                                                                                                               ccATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACgtcagt                                                                                                  >  1:469177/1‑55 (MQ=255)                                                                                                               ccATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACgtcagt                                                                                                  <  2:469177/55‑1 (MQ=255)                                                                                                               ccATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGtt                                                                                             <  1:90906/60‑1 (MQ=255)                                                                                                               ccATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGtt                                                                                             >  2:90906/1‑60 (MQ=255)                                                                                                               ccATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGtt                                                             >  2:932615/1‑92 (MQ=255)                                                                                                               ccATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGtt                                                             <  1:932615/92‑1 (MQ=255)                                                                                                               ccATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTg                                                >  1:529743/1‑105 (MQ=255)                                                                                                               ccATCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTg                                                <  2:529743/105‑1 (MQ=255)                                                                                                                 aTCCAACACTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTg            >  2:657625/1‑139 (MQ=255)                                                                                                                       cacTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAg                                        <  1:648321/105‑1 (MQ=255)                                                                                                                       cacTGAGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAg                                        >  2:648321/1‑105 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTAtcatca                                                                                                         <  1:1140989/35‑1 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTAtcatca                                                                                                         >  2:1140989/1‑35 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGt                                                          <  2:254515/82‑1 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGt                                                          >  1:254515/1‑82 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAAc                             <  2:1099277/111‑1 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAAc                             >  1:1099277/1‑111 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  2:629172/1‑138 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  2:524783/1‑138 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  2:479893/1‑138 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  2:860753/1‑138 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  2:863108/1‑138 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcgc  >  1:131182/1‑138 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTgcg   <  2:528187/137‑1 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTg     <  1:896390/135‑1 (MQ=255)                                                                                                                            aGTGAAAGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTg     >  2:896390/1‑135 (MQ=255)                                                                                                                                 aaGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGtgctg                                   >  2:504262/1‑100 (MQ=255)                                                                                                                                 aaGCGAGTTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGtgctg                                   <  1:504262/100‑1 (MQ=255)                                                                                                                                       |                                                                                                                               GTCGCGTTGGCGTGGTTAGCGTTCGCCATTCTGCGTCCAGGATCGGATGCTCGTAAAAGCCGCCGCCATATTCGCGCGCTGCGCCGGGATTTTGTCGATCAGCTAAGCCGCCATCCAACACTGAGTGAAAGCGAATTTGAATCGCTCACTTATCATCACGTCAGTCAGTTGAGTAACAGCCAGGATGCGCTGGCTCGCCGTTGGTTATTACGCTGGGGTGTAGTGCTGCTGAACTGTTCTCATGTTGTCTGGCAATTGCGC  >  NZ_CP009273/1717875‑1718135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑