Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,752,926	C→T	Q19Q (CAG→CAA)	ldtE ←	L,D‑transpeptidase LdtE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,752,926	0	C	T	98.8%	324.5 / ‑4.2	84	Q19Q (CAG→CAA)	ldtE	L,D‑transpeptidase LdtE
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (42/41);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

CAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCAAAACACTC  >  NZ_CP009273/1752791‑1753060                                                                                                                                        |                                                                                                                                       cAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTg                                                                                                                                      >  2:410421/1‑138 (MQ=255)   aCATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGAt                                                                                                                                   <  2:116094/139‑1 (MQ=255)   aCATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGAt                                                                                                                                   <  2:3246/139‑1 (MQ=255)         ccGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGgcgc                                                                                                                              >  2:914924/1‑138 (MQ=255)         ccGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGgcgc                                                                                                                              >  2:852668/1‑138 (MQ=255)         ccGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGgcgc                                                                                                                              >  2:172683/1‑138 (MQ=255)              gTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAg                                                                                                                        >  2:772245/1‑139 (MQ=255)                     aaCGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGAt                                                                                                                 <  1:852668/139‑1 (MQ=255)                       cGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGAt                                                                                                                 >  2:456183/1‑137 (MQ=255)                         tCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGc                                                                                                             >  1:382237/1‑139 (MQ=255)                           gggCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCg                                                                                                            <  2:966184/138‑1 (MQ=255)                           gggCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCg                                                                                                            <  1:456183/138‑1 (MQ=255)                           gggCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCg                                                                                                            <  1:89250/138‑1 (MQ=255)                           gggCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCg                                                                                                            <  1:967973/138‑1 (MQ=255)                                  aGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAgtgt                                                                                                     >  1:27746/1‑138 (MQ=255)                                    ccTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAg                                                                                                  >  1:1133497/1‑139 (MQ=255)                                        aaGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCa                                                                                                >  2:371288/1‑137 (MQ=255)                                              tttATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGAcgcg                                                                                        >  1:1227836/1‑139 (MQ=255)                                               ttATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGAcgcg                                                                                        <  2:818687/138‑1 (MQ=255)                                                    cccTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGttt                                                                                   <  2:27746/138‑1 (MQ=255)                                                            gCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTaa                                                                           <  2:401235/138‑1 (MQ=255)                                                            gCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTa                                                                            <  1:808713/137‑1 (MQ=255)                                                             cACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAc                                                                         <  1:371288/139‑1 (MQ=255)                                                             cACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAc                                                                         <  2:717154/139‑1 (MQ=255)                                                             cACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAc                                                                         <  1:746726/139‑1 (MQ=255)                                                                cGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAcctc                                                                      <  1:914924/139‑1 (MQ=255)                                                                cGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAcctc                                                                      >  1:1092425/1‑139 (MQ=255)                                                                  tataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGAt                                                                                                                 >  2:588150/1‑94 (MQ=255)                                                                  tataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGAt                                                                                                                 <  1:588150/94‑1 (MQ=255)                                                                  tataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCAt                                                                                <  2:1267972/127‑1 (MQ=255)                                                                  tataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCAt                                                                                >  1:1267972/1‑127 (MQ=255)                                                                  tataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAcctcct                                                                    >  2:1285502/1‑139 (MQ=255)                                                                  tataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAcctcct                                                                    >  2:427233/1‑139 (MQ=255)                                                                  tataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAcctcct                                                                    >  2:212265/1‑139 (MQ=255)                                                                  tataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAcctcct                                                                    >  1:408078/1‑139 (MQ=255)                                                                  tataCGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAAcctcct                                                                    >  1:850469/1‑139 (MQ=255)                                                                      cGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTAt                                                                <  1:172683/139‑1 (MQ=255)                                                                          tGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGtt                                                                                    <  1:239656/115‑1 (MQ=255)                                                                          tGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGtt                                                                                    >  2:239656/1‑115 (MQ=255)                                                                          tGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGc                                                            >  2:788325/1‑139 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGAt                                                                                                                 >  2:677673/1‑84 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGAt                                                                                                                 <  1:677673/84‑1 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTa                                                                            >  2:366064/1‑121 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCg                                                          >  1:250781/1‑139 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCg                                                          >  2:892830/1‑139 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCg                                                          >  1:977026/1‑139 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCg                                                          >  1:1165945/1‑139 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCg                                                          >  1:1077737/1‑139 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTAGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTACGTGCCg                                                          >  1:769174/1‑139 (MQ=255)                                                                            aTTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCg                                                          >  2:5258/1‑139 (MQ=255)                                                                                 gCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGAc                                                     >  2:393593/1‑139 (MQ=255)                                                                                  cccAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGt                                                                                                           <  1:935194/84‑1 (MQ=255)                                                                                  cccAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGt                                                                                                           >  2:935194/1‑84 (MQ=255)                                                                                  cccAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGAc                                                     <  1:788325/138‑1 (MQ=255)                                                                                  cccAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACt                                                    <  1:212265/139‑1 (MQ=255)                                                                                    cAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCa                                                  <  2:34738/139‑1 (MQ=255)                                                                                        cAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCt                                              <  1:410421/139‑1 (MQ=255)                                                                                         aGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTg                                             <  1:427233/139‑1 (MQ=255)                                                                                            cGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTa                                                                            <  2:112221/105‑1 (MQ=255)                                                                                            cGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTa                                                                            >  1:112221/1‑105 (MQ=255)                                                                                                   cGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGa                                   <  1:839038/139‑1 (MQ=255)                                                                                                    ggTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGAt                                  <  2:850469/139‑1 (MQ=255)                                                                                                     gTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATg                                 >  2:704253/1‑139 (MQ=255)                                                                                                      ttGGTGGTAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATgg                                <  1:598734/139‑1 (MQ=255)                                                                                                         gtggtAGCGGATAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGgcgc                             >  1:792943/1‑139 (MQ=255)                                                                                                                    tAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGt                                                                                                           <  2:599944/50‑1 (MQ=255)                                                                                                                    tAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGt                                                                                                           >  1:599944/1‑50 (MQ=255)                                                                                                                    tAATCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCAcc                  >  1:1253677/1‑139 (MQ=255)                                                                                                                       tCAACCGCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGa               <  1:335453/139‑1 (MQ=255)                                                                                                                             gCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGtt                                                                                    >  2:924552/1‑64 (MQ=255)                                                                                                                             gCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGtt                                                                                    <  1:924552/64‑1 (MQ=255)                                                                                                                             gCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGa               >  1:269786/1‑133 (MQ=255)                                                                                                                             gCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGa               <  2:269786/133‑1 (MQ=255)                                                                                                                             gCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCa          >  1:576432/1‑138 (MQ=255)                                                                                                                             gCCCAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCa          >  1:275617/1‑138 (MQ=255)                                                                                                                              cccAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGacaaaaa         >  1:354649/1‑132 (MQ=255)                                                                                                                              cccAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCaa         >  1:718724/1‑138 (MQ=255)                                                                                                                               ccAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCaaaa       <  2:1253677/139‑1 (MQ=255)                                                                                                                               ccAGGCAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCaaaa       <  2:718724/139‑1 (MQ=255)                                                                                                                                 agtcAGCTTGTATGGCTCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCaaaa       <  1:366064/134‑1 (MQ=255)                                                                                                                                    cAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCAAAACACTc  <  1:393593/139‑1 (MQ=255)                                                                                                                                    cAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCAAAACACTc  <  2:250781/139‑1 (MQ=255)                                                                                                                                    cAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCAAAACACTc  <  2:382237/139‑1 (MQ=255)                                                                                                                                    cAGCTTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCAAAACACTc  <  2:576432/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCAAAACACTC  >  NZ_CP009273/1752791‑1753060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑