| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,820,976 | C→T | A69T (GCG→ACG) | astE ← | succinylglutamate desuccinylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,820,976 | 0 | C | T | 92.6% | 176.5 / 3.4 | 54 | A69T (GCG→ACG) | astE | succinylglutamate desuccinylase |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base T (19/31); total (21/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
GCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAA > NZ_CP009273/1820847‑1821110 | gCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCaa < 2:513010/139‑1 (MQ=255) cTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAAc > 1:1143477/1‑139 (MQ=255) aCGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTcc < 2:804348/139‑1 (MQ=255) aCGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTGGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTcc < 2:1043393/139‑1 (MQ=255) gACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAg > 2:752287/1‑139 (MQ=255) aCCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGc < 1:78131/139‑1 (MQ=255) aCCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGc < 1:524301/139‑1 (MQ=255) aCCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGc < 2:760311/139‑1 (MQ=255) ccAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCAtct < 2:1271872/139‑1 (MQ=255) ccAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCAtct > 1:837897/1‑139 (MQ=255) aaCATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCca < 1:53762/139‑1 (MQ=255) aCATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCcac < 2:85584/139‑1 (MQ=255) gATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc < 1:51266/139‑1 (MQ=255) gATTCATATCGCTATGGCAAAAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc < 2:199541/139‑1 (MQ=255) aTTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGcc < 2:346032/139‑1 (MQ=255) tatCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtct > 2:391333/1‑138 (MQ=255) gCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTa < 1:197838/139‑1 (MQ=255) gCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTa < 1:148758/139‑1 (MQ=255) tATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAcc > 1:475423/1‑139 (MQ=255) aTAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTAtt > 1:537016/1‑139 (MQ=255) tAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTc > 1:487424/1‑139 (MQ=255) cGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCg < 1:1237297/139‑1 (MQ=255) cGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCg < 2:487424/139‑1 (MQ=255) tCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTg > 2:298900/1‑138 (MQ=255) ccTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGaa < 1:752287/138‑1 (MQ=255) gaggaTTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCAt < 2:465716/82‑1 (MQ=255) gaggaTTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCAt > 1:465716/1‑82 (MQ=255) gaggaTTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTg < 2:837897/139‑1 (MQ=255) aggaTTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGc < 2:537016/139‑1 (MQ=255) ggaTTCCCGAGGATCCCCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCg < 1:981752/139‑1 (MQ=255) gaTTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCgg < 2:475423/139‑1 (MQ=255) ttCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc < 1:93721/89‑1 (MQ=255) ttCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc > 2:93721/1‑89 (MQ=255) cccGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTgg < 1:298900/138‑1 (MQ=255) gAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGt > 2:574313/1‑138 (MQ=255) ccAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGt < 1:680931/139‑1 (MQ=255) aCCGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTc > 2:885869/1‑139 (MQ=255) cGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa < 2:307766/75‑1 (MQ=255) cGCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa > 1:307766/1‑75 (MQ=255) gCCAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAg > 2:900697/1‑139 (MQ=255) cAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAAc > 2:1175856/1‑42 (MQ=255) cAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAAc < 1:1175856/42‑1 (MQ=255) cAACGTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGca < 2:944545/139‑1 (MQ=255) gTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACAcc < 2:1143477/139‑1 (MQ=255) gTAACGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACAcc < 2:101213/139‑1 (MQ=255) aaCGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTc < 2:334198/123‑1 (MQ=255) aaCGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTc > 1:334198/1‑123 (MQ=255) aCGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCAtc < 2:607841/47‑1 (MQ=255) aCGGGATCTCGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCAtc > 1:607841/1‑47 (MQ=255) tctcGCCGTGAGATATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCccag > 2:494967/1‑139 (MQ=255) agaTATCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAgccagcgccag > 1:1263501/1‑139 (MQ=255) agaTATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAgccagcgccag > 1:834567/1‑139 (MQ=255) atatCGTGCCAAGCAACGCGTCCAGCATCTACACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGCGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAgccagcgccagc > 2:288422/1‑138 (MQ=255) tCGTGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGaa < 1:880632/138‑1 (MQ=255) | GCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAA > NZ_CP009273/1820847‑1821110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |