| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,913,346 | T→G | V527G (GTG→GGG) | yebT → | lipid‑binding membrane homeostasis protein YebT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,913,346 | 0 | T | G | 97.8% | 165.2 / ‑3.4 | 46 | V527G (GTG→GGG) | yebT | lipid‑binding membrane homeostasis protein YebT |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (20/25); total (20/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01 | |||||||||||
ATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGC > NZ_CP009273/1913213‑1913479 | aTTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcagg > 2:1105181/1‑139 (MQ=255)aTTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcagg > 2:536332/1‑139 (MQ=255) ttCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcaggc < 1:551974/139‑1 (MQ=255) ttCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcaggc < 1:441351/139‑1 (MQ=255) ttCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcagg < 2:1285447/138‑1 (MQ=255) ccGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGcaggcagg < 2:1083790/137‑1 (MQ=255) gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCg > 2:1046297/1‑139 (MQ=255) ggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCgg > 2:1018231/1‑139 (MQ=255) ggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCgg < 1:1134700/139‑1 (MQ=255) ggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCgg > 1:77639/1‑139 (MQ=255) ggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCgg < 1:1105181/139‑1 (MQ=255) tGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTAc > 1:411406/1‑139 (MQ=255) gCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGt > 2:389889/1‑139 (MQ=255) gCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGt > 2:790888/1‑139 (MQ=255) tCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGt < 2:761638/139‑1 (MQ=255) tCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGt < 1:501262/139‑1 (MQ=255) cACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGa < 2:798904/139‑1 (MQ=255) tATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcaggc < 1:1200277/95‑1 (MQ=255) tATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcaggc > 2:1200277/1‑95 (MQ=255) gCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTa > 1:695740/1‑139 (MQ=255) tttGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGcaggcagg > 1:1218692/1‑90 (MQ=255) tttGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGcaggcagg < 2:1218692/90‑1 (MQ=255) tGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcaggc < 1:45447/85‑1 (MQ=255) tGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGgcaggc > 2:45447/1‑85 (MQ=255) tGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCg > 2:756140/1‑139 (MQ=255) tGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCg > 1:1271519/1‑139 (MQ=255) gAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTc > 2:756802/1‑139 (MQ=255) aaaaaGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTcc < 1:1046297/139‑1 (MQ=255) gagaACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAAc < 1:262586/138‑1 (MQ=255) gaACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAAcgcg < 1:1018231/139‑1 (MQ=255) cAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGttt > 2:989968/1‑139 (MQ=255) cTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGata < 1:774519/139‑1 (MQ=255) gCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCg < 2:357726/138‑1 (MQ=255) aTTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCt < 2:889095/139‑1 (MQ=255) acaACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGc < 2:1052592/139‑1 (MQ=255) aaCCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCg > 2:183419/1‑139 (MQ=255) aCCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCgg < 1:790888/139‑1 (MQ=255) aCCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCgg < 1:389889/139‑1 (MQ=255) aGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATc > 2:1236886/1‑139 (MQ=255) aGTTTGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATc > 2:52133/1‑139 (MQ=255) tGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCa < 2:411406/138‑1 (MQ=255) tGAGTGCAGAGACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCa < 1:1006436/138‑1 (MQ=255) agagACGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTg < 1:536332/139‑1 (MQ=255) agaCGCTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGAc < 1:756140/139‑1 (MQ=255) cTGCCGGATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGc > 2:30800/1‑138 (MQ=255) ggATGGGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGc < 1:407077/139‑1 (MQ=255) | ATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGC > NZ_CP009273/1913213‑1913479 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |