| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 123,341 | T→A | L387Q (CTG→CAG) | aceF → | pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 123,341 | 0 | T | A | 95.5% | 147.4 / ‑0.8 | 44 | L387Q (CTG→CAG) | aceF | pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base A (29/13); total (30/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
AACCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACA > NZ_CP009273/123208‑123472 | aaCCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGGAGCCgg > 2:1258051/1‑138 (MQ=255) tGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGGCGTGGCCg > 2:325746/1‑139 (MQ=255) tGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCAGCCGTGGcc > 1:126922/1‑138 (MQ=255) tGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCAGCCGTGGCCGAAGGTGGa > 1:661399/1‑139 (MQ=255) gCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAg < 1:356128/139‑1 (MQ=255) aaaGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGt > 1:1269552/1‑138 (MQ=255) cGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATc > 1:588100/1‑138 (MQ=255) gTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAAttg > 2:129887/1‑136 (MQ=255) gTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAACTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCg > 2:1141920/1‑138 (MQ=255) gcgcGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGGAGGTGGACTTCAGCAAGGTTGGTGAAATCGAAGAAGTg > 2:655359/1‑139 (MQ=255) gcgcGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGaagaag > 1:1038934/1‑137 (MQ=255) gcgcGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGaagaag < 2:1038934/137‑1 (MQ=255) gcgcGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTg > 1:1199542/1‑139 (MQ=255) gcgcGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGGGGACTTCAGCAAGGTTGGTGAAATCGAAGAAGTg > 1:1099570/1‑139 (MQ=255) cgcgAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGGGGTATCCCTGGCATGCAGCCCTGGGCGCAGGTGGACTTCAGCTAGTTTTGGGAAATCGAAGAATTg > 1:840289/1‑138 (MQ=255) ttCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGCAGGGGGACTTCAGCCAGTTTGGTGAAATCGAAGAAGGGGGACTGGGCCg > 2:437780/1‑139 (MQ=255) ggCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATc < 1:1233740/139‑1 (MQ=255) aCGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGGAGGTGGACTTCAGCAAGTTTGGGGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGa > 2:389715/1‑138 (MQ=255) aagaagCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAAtctc > 1:751773/1‑139 (MQ=255) aagaagCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAAtctc > 1:800335/1‑139 (MQ=255) agCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGt > 2:475872/1‑139 (MQ=255) agCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGGGGTATCCCTGGCATGCAGAAGTGGCCCTAGGGGGGCTTCCGCAAGATTGGTGCAATCGAAGAAGTGGAACTGGGTCGCATTCGGAAAATCTCTGGt > 2:115900/1‑139 (MQ=255) gCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTg < 2:1269552/139‑1 (MQ=255) cAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCg < 1:577329/139‑1 (MQ=255) aGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCg > 1:949519/1‑138 (MQ=255) aaGCAGCTCCGGCAGCGACTGGCGGGGGAATCCCAGGCATGCACGCCTGGGCGAAGGTGGCCTTCAGCCAGGTTGGGGAAATCGAAAAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTaa > 2:431797/1‑139 (MQ=255) gcTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTggg > 1:220040/1‑139 (MQ=255) cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTg > 2:368008/1‑117 (MQ=255) cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTg < 2:310192/117‑1 (MQ=255) cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTg > 1:310192/1‑117 (MQ=255) cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTg < 1:368008/117‑1 (MQ=255) cTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGGGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTggg > 1:934649/1‑138 (MQ=255) cGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATccc < 1:325746/139‑1 (MQ=255) cGACTGGCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATccc < 2:220040/139‑1 (MQ=255) gCGGTGGTATCCCTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATg < 2:751773/138‑1 (MQ=255) cccTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAAtctc > 1:1055114/1‑87 (MQ=255) cccTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAAtctc < 2:1055114/87‑1 (MQ=255) cccTGGCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTc > 2:1046711/1‑139 (MQ=255) ggCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAAtctc < 2:446541/83‑1 (MQ=255) ggCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAAtctc > 1:446541/1‑83 (MQ=255) ggCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGAc > 2:146818/1‑138 (MQ=255) gCATGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACa < 2:911748/138‑1 (MQ=255) aTGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCt > 2:1174449/1‑92 (MQ=255) aTGCAGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCt < 1:1174449/92‑1 (MQ=255) tgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGaagaag < 2:374872/48‑1 (MQ=255) tgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGaagaag > 1:374872/1‑48 (MQ=255) | AACCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGATCCCGCATGTTACTCACTTCGACA > NZ_CP009273/123208‑123472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |