| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,977,975 | C→T | E192E (GAG→GAA) | araG ← | arabinose ABC transporter ATP‑binding protein AraG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,977,975 | 0 | C | T | 90.3% | 198.3 / 9.6 | 62 | E192E (GAG→GAA) | araG | arabinose ABC transporter ATP‑binding protein AraG |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base T (32/24); total (35/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACT > NZ_CP009273/1977840‑1978108 | cACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCttct > 1:982428/1‑139 (MQ=255) gcgcGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCg > 2:609320/1‑139 (MQ=255) gcgcGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCg > 1:356741/1‑139 (MQ=255) cgcgTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGc < 2:756583/139‑1 (MQ=255) aCCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATaa > 2:918198/1‑139 (MQ=255) tgcATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCgg > 1:584457/1‑138 (MQ=255) gTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGt > 1:661950/1‑139 (MQ=255) ttGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCAc > 1:122381/1‑139 (MQ=255) ttGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCAc > 2:543670/1‑139 (MQ=255) gACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCa > 2:492516/1‑136 (MQ=255) gACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCa < 1:492516/136‑1 (MQ=255) aTAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCa < 2:133789/139‑1 (MQ=255) aaCGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCa > 2:776692/1‑137 (MQ=255) aaCGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCa > 1:619009/1‑137 (MQ=255) aCGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCagag < 2:633207/139‑1 (MQ=255) aTCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAg < 2:619009/139‑1 (MQ=255) tAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTgg < 2:1203904/138‑1 (MQ=255) tAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTgg < 1:776692/138‑1 (MQ=255) aGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtca > 1:1127365/1‑139 (MQ=255) aGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtca > 1:867994/1‑139 (MQ=255) aGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtca > 2:969539/1‑139 (MQ=255) aGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtca > 1:387363/1‑139 (MQ=255) gTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtcat > 2:492825/1‑139 (MQ=255) gTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCtcat > 1:714449/1‑139 (MQ=255) cATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCa > 2:186585/1‑104 (MQ=255) cATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCa < 1:186585/104‑1 (MQ=255) tCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACg > 2:449739/1‑104 (MQ=255) tCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACg < 1:449739/104‑1 (MQ=255) tCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGa < 1:543670/139‑1 (MQ=255) tCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGa > 2:307066/1‑139 (MQ=255) agacaggggCAAATTTTTCTCCCATGCGGTGAAAAACGTATAAGATTCCCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGAt < 2:1231458/133‑1 (MQ=255) gCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCAc > 2:189588/1‑101 (MQ=255) gCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCAc < 1:189588/101‑1 (MQ=255) gCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGAt > 1:569326/1‑138 (MQ=255) gCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGAt > 2:163835/1‑138 (MQ=255) gCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTgg < 2:341550/139‑1 (MQ=255) atatTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGt > 1:870210/1‑138 (MQ=255) tcttcCATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTAcgcg > 1:544685/1‑139 (MQ=255) tcttcCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGtctgtct > 1:1231458/1‑133 (MQ=255) tcCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACgcgc > 2:161754/1‑137 (MQ=255) ccATACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACgcgc < 1:1225405/136‑1 (MQ=255) tACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCa < 2:670738/77‑1 (MQ=255) tACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCa > 1:670738/1‑77 (MQ=255) tACGGTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAgcgc > 1:1185094/1‑139 (MQ=255) gTGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTg < 2:621378/139‑1 (MQ=255) tGAGAAACGTATAAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTgg > 2:257630/1‑139 (MQ=255) tataAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCaa < 1:180978/139‑1 (MQ=255) tataAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCaa < 1:1276493/139‑1 (MQ=255) ataAGATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAAc > 1:503180/1‑139 (MQ=255) aGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCAt < 1:163835/139‑1 (MQ=255) gATTACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGGCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATc < 1:927147/139‑1 (MQ=255) tACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGc > 1:749348/1‑60 (MQ=255) tACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGc < 2:749348/60‑1 (MQ=255) tACCCGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGc > 1:791807/1‑139 (MQ=255) cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAg < 1:858636/68‑1 (MQ=255) cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAg > 2:858636/1‑68 (MQ=255) cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCa > 2:477162/1‑139 (MQ=255) cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCa > 2:1088872/1‑139 (MQ=255) cccGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCa < 1:307066/139‑1 (MQ=255) cccGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCa < 1:161754/139‑1 (MQ=255) cGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACt < 2:122381/139‑1 (MQ=255) cGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACt < 2:870210/139‑1 (MQ=255) cGCCCTTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACt < 1:1088872/139‑1 (MQ=255) | CACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCAGTTCACGAATAACGCGGAAAAGATTGTCGATTTCACGGGCAGAGAGGGAGCTGGTTGGCTCATCAAAGGCGATAATTTTGGCGTTACGCGCCAGCGCTTTGGCGATTTCAACCATCTGCCACT > NZ_CP009273/1977840‑1978108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |