Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,014,277 A→G E237G (GAA→GGA)  fliM → flagellar motor switch protein FliM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,014,2770AG100.0% 294.9 / NA 79E237G (GAA→GGA) fliMflagellar motor switch protein FliM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/35);  total (44/35)

CGCCGAACGACATTGTGGTTAA‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGG  >  NZ_CP009273/2014148‑2014409
                                                                                                                                  |                                                                                                                                    
cGCCGAACGACATTGTGGTTAA‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTcgcg                                                                                                                             >  1:377773/1‑139 (MQ=255)
cGCCGAACGACATTGTGGTTAA‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTcgcg                                                                                                                             >  2:928632/1‑139 (MQ=255)
cGCCGAACGACATTG‑GGTTAACCAGGCCGTTCCATGTGGAGATTGACAACCTGCCCGGCAAATTAAATACCTGCCTCCAATCCAGCAGGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTcgcg                                                                                                                             <  2:654107/139‑1 (MQ=255)
 gCCGAACGACATTGTGGTTAA‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTGCGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTcgcg                                                                                                                             >  2:710447/1‑138 (MQ=255)
     aacaacTTTGTGGTTAC‑ACCGCCGTCCCATGTGGAGATTGGCACCCGGCCGGGCAATTTAAATACCTCCCTCCCTTCCAGCATAACCGGGCCGCTAGGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATg                                                                                                                        <  2:858734/139‑1 (MQ=255)
     aaCGACTTTGTGTTAAA‑CACGCCGTTCCATGTGGAGATTGGCAACCTGCCCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATg                                                                                                                        <  1:63655/139‑1 (MQ=255)
     aaCGACATTGTGTTTAA‑CACGCCTTTCCATGTGGAGATTGGCAACCTGACCGGCAAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGTTTAACCCGCCGCTGGGAAACTCGCGTAATg                                                                                                                        <  1:775735/139‑1 (MQ=255)
         aCATTGTGGTTAA‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATg                                                                                                                        >  2:922085/1‑135 (MQ=255)
               tgGTTAA‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGaa                                                                                                              >  1:1033769/1‑139 (MQ=255)
                    aa‑CACGCCGTTCCATGTGGGGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGgc                                                                                                         >  2:438935/1‑139 (MQ=255)
                    aa‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGgc                                                                                                         >  2:805268/1‑139 (MQ=255)
                    aa‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGgc                                                                                                         >  1:356335/1‑139 (MQ=255)
                    aa‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGgc                                                                                                         >  2:1078516/1‑139 (MQ=255)
                        acGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTGCGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGcgcg                                                                                                      <  1:710447/139‑1 (MQ=255)
                          gCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGa                                                                                                     >  1:1165838/1‑138 (MQ=255)
                          gCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGa                                                                                                     >  1:603862/1‑138 (MQ=255)
                          gCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGa                                                                                                     >  2:452467/1‑138 (MQ=255)
                            cGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTcg                                                                                                                               >  1:553053/1‑110 (MQ=255)
                            cGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTcg                                                                                                                               <  2:553053/110‑1 (MQ=255)
                               tCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCt                                                                                               >  1:301710/1‑139 (MQ=255)
                                 cATGTGGAGATTGGCACCCTGCCCGGCAAATTTAATATCTGCCTGCCATTCAGCATGATCGGGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTgg                                                                                             <  2:1033769/139‑1 (MQ=255)
                                     tgGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTgcg                                                                                         <  2:636010/139‑1 (MQ=255)
                                     gggAGATTGGCACCCTGCCCGGCGATTTTAATATCTGCCTCCCATTCAGCATGATGGAGCCGCTCCGGGATTTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTgcg                                                                                         <  2:1165838/138‑1 (MQ=255)
                                       gagaTTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGcc                                                                                       <  1:153225/139‑1 (MQ=255)
                                       gagaTTGGAACCCGGCCCGGCAATTTAAATACCGCCCTGCCATCCAGCAGGATCGGGCCGCTGGGGGATTTGTTGGTTACCCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGcc                                                                                       <  2:19862/139‑1 (MQ=255)
                                        agaTTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTgcgc                                                                                        >  1:894482/1‑137 (MQ=255)
                                          aTTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAgg                                                                                    >  2:968868/1‑139 (MQ=255)
                                          aTTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAgg                                                                                    >  1:231539/1‑139 (MQ=255)
                                           ttGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGt                                                                                   >  1:557278/1‑139 (MQ=255)
                                             ggCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGcc                                                                                       >  2:632565/1‑133 (MQ=255)
                                             ggCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGcc                                                                                       <  1:632565/133‑1 (MQ=255)
                                             ggCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTg                                                                                  >  2:333337/1‑138 (MQ=255)
                                             ggCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTg                                                                                  >  1:749226/1‑138 (MQ=255)
                                              gCACCCTGCCGGGCGAATTAAATATCTGCCTGCCTTTCAGCATGATCGGGCCGCTACGGGAATTGTTGGTTACCCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgca                                                                                <  2:894482/139‑1 (MQ=255)
                                               cAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgaag                                                                               >  2:774454/1‑139 (MQ=255)
                                                 cccTGACCGGCGAATTAAATATCTGCCTGCCATTCAGCATGATCGGGCCGCTACGGGAATTGTGGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgcagca                                                                             <  2:34324/138‑1 (MQ=255)
                                                 aCCTGACCGGCGAATTTAATATCTGCCTGCCAGTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgcagca                                                                             >  1:1116140/1‑139 (MQ=255)
                                                   cggACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAtt                                                                           <  1:688102/137‑1 (MQ=255)
                                                   cTGACCGGCGATTTTAATATCAGCCTGCCATCCAGCATGATCGAGCCGCTCCGGGAATTGTTGTTGAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAtt                                                                           <  1:244702/139‑1 (MQ=255)
                                                      aCCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTAGGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTca                                                                         <  2:356335/138‑1 (MQ=255)
                                                      aCCGGCAATTTAAATTTCTGCCTGCCATTCAGCATGACCGACCCGCTAGGGAATTTGTTGGTTACCCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTc                                                                          <  2:749226/137‑1 (MQ=255)
                                                         ggCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACagc                                                                     <  1:805268/139‑1 (MQ=255)
                                                                ttAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACagctggagct                                                              >  2:1030349/1‑139 (MQ=255)
                                                                         gcctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTc                                                                          >  2:531507/1‑118 (MQ=255)
                                                                         gcctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTc                                                                          <  1:531507/118‑1 (MQ=255)
                                                                         gcctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGc                                                        <  2:393001/136‑1 (MQ=255)
                                                                         gcctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGc                                                        >  1:393001/1‑136 (MQ=255)
                                                                         gcctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCaa                                                     >  2:686704/1‑139 (MQ=255)
                                                                          cctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACt                                                                                                            <  2:1028242/83‑1 (MQ=255)
                                                                          cctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACt                                                                                                            >  1:1028242/1‑83 (MQ=255)
                                                                          cctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAAc                                                    >  2:331679/1‑139 (MQ=255)
                                                                                 tccAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCg                                             <  1:1030349/137‑1 (MQ=255)
                                                                                  tCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGa                                            >  2:1240837/1‑139 (MQ=255)
                                                                                    aGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTgg                                                                                                          <  2:1219116/75‑1 (MQ=255)
                                                                                    aGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTgg                                                                                                          >  1:1219116/1‑75 (MQ=255)
                                                                                     tcgtGATCGAGCCGCTGCGGGATTTTTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGatat                                         <  1:922085/136‑1 (MQ=255)
                                                                                     gCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGatat                                         <  1:333337/139‑1 (MQ=255)
                                                                                           tCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGc                                    >  1:446313/1‑138 (MQ=255)
                                                                                              aGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGatat                                         <  2:767646/130‑1 (MQ=255)
                                                                                              aGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGatat                                         >  1:767646/1‑130 (MQ=255)
                                                                                              aGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACg                                >  2:484936/1‑139 (MQ=255)
                                                                                                ccGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTg                                                                                              >  2:449385/1‑75 (MQ=255)
                                                                                                ccGCTACGGGAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTg                                                                                              <  1:449385/75‑1 (MQ=255)
                                                                                                       gggAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCa                       <  2:231539/139‑1 (MQ=255)
                                                                                                       gggAATTGTTGGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCa                       <  1:1078516/139‑1 (MQ=255)
                                                                                                             tgttgGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAtt                                                                           >  2:494811/1‑81 (MQ=255)
                                                                                                             tgttgGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAtt                                                                           <  1:494811/81‑1 (MQ=255)
                                                                                                              gttgGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGAtttt                 >  1:1139412/1‑138 (MQ=255)
                                                                                                                tgGTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTaa               >  2:35881/1‑138 (MQ=255)
                                                                                                                  gTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGAt                                                                                                                   <  2:984375/36‑1 (MQ=255)
                                                                                                                  gTTAACCCGCCGCTGGGAAACTCGCGTAATGAAGAt                                                                                                                   >  1:984375/1‑36 (MQ=255)
                                                                                                                     aaCCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTg          <  1:452467/138‑1 (MQ=255)
                                                                                                                     aaCCCGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTg          >  1:331727/1‑138 (MQ=255)
                                                                                                                       cccGCCGCTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAAc       >  1:114481/1‑139 (MQ=255)
                                                                                                                         cgccacTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACTTGGTGCGCCAAGTGCAGCATTCACAGCTCGAGCTAGTCGCAAGCTTAGCCTATATCACGCTACGCCTGTCGCAGATTTTAAAACTGAAccc     <  2:501428/139‑1 (MQ=255)
                                                                                                                           ccgcTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCg   >  1:1262969/1‑139 (MQ=255)
                                                                                                                            cgcTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCgg  >  2:800892/1‑139 (MQ=255)
                                                                                                                            cgcTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCgg  >  2:1101161/1‑139 (MQ=255)
                                                                                                                            cgcTGGGAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCgg  <  2:377773/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                    
CGCCGAACGACATTGTGGTTAA‑CACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGG  >  NZ_CP009273/2014148‑2014409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑