| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,115,376 | A→G | L362P (CTG→CCG) | cpsG ← | colanic acid biosynthesis phosphomannomutase CpsG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,115,376 | 0 | A | G | 95.7% | 144.2 / ‑1.7 | 46 | L362P (CTG→CCG) | cpsG | colanic acid biosynthesis phosphomannomutase CpsG |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (18/26); total (19/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACG > NZ_CP009273/2115242‑2115512 | tGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGt > 2:150214/1‑139 (MQ=255) aTCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTa > 2:684858/1‑139 (MQ=255) aTCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTa > 2:6813/1‑139 (MQ=255) aTCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTa > 2:221749/1‑139 (MQ=255) cAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAgg < 2:569468/139‑1 (MQ=255) cgcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGca > 1:1143243/1‑138 (MQ=255) tGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGAc > 1:188410/1‑137 (MQ=255) cTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagc < 1:448649/139‑1 (MQ=255) cTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcag < 1:1076548/138‑1 (MQ=255) cTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcag < 2:1200900/138‑1 (MQ=255) cTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcag < 2:1064477/138‑1 (MQ=255) gTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagcag > 2:689556/1‑139 (MQ=255) ccACGCGGTTAATCGCCTCAACGGGGTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCa > 1:407499/1‑139 (MQ=255) gTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATc < 1:1289891/139‑1 (MQ=255) ttAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCa > 2:852111/1‑139 (MQ=255) gCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCg < 1:942204/138‑1 (MQ=255) gCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCg < 2:407499/138‑1 (MQ=255) cTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTg > 2:924732/1‑139 (MQ=255) gcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGa < 1:715568/139‑1 (MQ=255) ttGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGaa < 2:816394/137‑1 (MQ=255) cGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggt > 1:486824/1‑135 (MQ=255) cGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggt < 2:486824/135‑1 (MQ=255) cGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGgc > 2:591786/1‑139 (MQ=255) tGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCt > 1:683529/1‑138 (MQ=255) aaaCGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCg < 1:719089/139‑1 (MQ=255) aaaCGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCg < 2:357734/139‑1 (MQ=255) aaaCGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCg < 2:188410/139‑1 (MQ=255) aCGCCGCCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGcc < 2:773798/139‑1 (MQ=255) cgccgcCATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGcca > 2:91562/1‑139 (MQ=255) cgccATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGccacca > 2:198783/1‑139 (MQ=255) cctCCGGCCGAGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCCCCATAg < 2:360202/137‑1 (MQ=255) cATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAg < 1:6813/139‑1 (MQ=255) cATCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAg < 1:924732/139‑1 (MQ=255) aTCCGGTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGa < 1:1263827/139‑1 (MQ=255) gTCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCg < 1:852111/139‑1 (MQ=255) tCGCGTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGt < 2:937205/139‑1 (MQ=255) cgTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCg > 2:206452/1‑72 (MQ=255) cgTACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCg < 1:206452/72‑1 (MQ=255) tACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTcc > 2:890272/1‑139 (MQ=255) tACCAGTTCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTcc < 1:591786/139‑1 (MQ=255) tACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTcc > 2:735149/1‑139 (MQ=255) ttCGCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTgcgc < 2:683529/139‑1 (MQ=255) gCCCGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATa < 2:1230440/139‑1 (MQ=255) cccGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGaa < 1:1232783/86‑1 (MQ=255) cccGGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGaa > 2:1232783/1‑86 (MQ=255) ccAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACg < 1:735149/139‑1 (MQ=255) | TGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACG > NZ_CP009273/2115242‑2115512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |