| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,121,319 | G→A | V159V (GTC→GTT) | gmd ← | GDP‑mannose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,121,319 | 0 | G | A | 98.2% | 214.5 / ‑4.2 | 57 | V159V (GTC→GTT) | gmd | GDP‑mannose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (25/31); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
TTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGC > NZ_CP009273/2121183‑2121450 | ttGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAAc < 1:1148903/139‑1 (MQ=255) tGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAAc < 2:242361/138‑1 (MQ=255) tGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAAc > 1:242361/1‑138 (MQ=255) gTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACg < 1:30921/139‑1 (MQ=255) tGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACgg < 1:863695/139‑1 (MQ=255) ttGCGGGTAACGAAGGTTTCGCCGCGGCGC‑GGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATc < 1:474953/138‑1 (MQ=255) tGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATc < 2:297126/138‑1 (MQ=255) tGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATc < 1:160297/138‑1 (MQ=255) gggTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATggc > 2:86597/1‑137 (MQ=255) gggTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATcgc < 1:86597/137‑1 (MQ=255) gTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCg < 1:301775/139‑1 (MQ=255) gTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCg < 1:1160148/139‑1 (MQ=255) ttCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTg > 2:340637/1‑139 (MQ=255) tCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTgg > 1:1149954/1‑139 (MQ=255) cggcgcggGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCtt > 2:345107/1‑139 (MQ=255) ggATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGa < 2:128692/139‑1 (MQ=255) aTGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTcc < 1:1079756/139‑1 (MQ=255) aTGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTcc < 2:414028/139‑1 (MQ=255) aTGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc < 1:1179208/138‑1 (MQ=255) aTGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc < 2:597838/138‑1 (MQ=255) aTGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc < 2:552088/138‑1 (MQ=255) ttGAAGAGAATTCCGTGACAGGCGTTCATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAGCGGCGTGGTCTCTTTCTGCGGAATTTACTgtg < 2:1052326/139‑3 (MQ=255) gAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCAc > 2:151100/1‑138 (MQ=255) ttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 2:1024591/139‑1 (MQ=255) ttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa > 1:916506/1‑139 (MQ=255) ttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:664820/139‑1 (MQ=255) ttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:340637/139‑1 (MQ=255) ttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa > 1:300368/1‑139 (MQ=255) ttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:151100/139‑1 (MQ=255) ggCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCagag > 1:648162/1‑139 (MQ=255) tAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCg < 2:1040513/79‑1 (MQ=255) tAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCg > 1:1040513/1‑79 (MQ=255) cACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAAc > 2:822403/1‑138 (MQ=255) aCGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATc > 1:87090/1‑56 (MQ=255) aCGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATc < 2:87090/56‑1 (MQ=255) aCGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa > 2:107717/1‑108 (MQ=255) aCGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:107717/108‑1 (MQ=255) aCGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAAc > 1:853271/1‑137 (MQ=255) cGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAg < 2:1035814/139‑1 (MQ=255) ttAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCg > 1:728239/1‑64 (MQ=255) ttAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCg < 2:1286751/64‑1 (MQ=255) ttAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCg > 1:1286751/1‑64 (MQ=255) ttAACGGTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCg < 2:728239/64‑1 (MQ=255) ggTGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttttcttttc > 1:536426/1‑138 (MQ=255) tGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtc < 1:826524/62‑1 (MQ=255) tGATCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtc > 2:826524/1‑62 (MQ=255) aTCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa > 2:528811/1‑82 (MQ=255) aTCCAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa < 1:528811/82‑1 (MQ=255) cAGTAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCg < 2:811091/138‑1 (MQ=255) tAGGCGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAgg > 1:114333/1‑138 (MQ=255) cGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCag < 1:1064964/90‑1 (MQ=255) cGTACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCag > 2:1064964/1‑90 (MQ=255) tACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGa > 1:1085336/1‑139 (MQ=255) tACAGTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGa > 2:916921/1‑139 (MQ=255) gTTTGGCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGc > 2:95856/1‑139 (MQ=255) ggCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTc < 2:1195723/78‑1 (MQ=255) ggCAACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTc > 1:1195723/1‑78 (MQ=255) | TTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGC > NZ_CP009273/2121183‑2121450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |